KEGG   ENZYME: 2.5.1.99Help
Entry
EC 2.5.1.99                 Enzyme                                 

Name
all-trans-phytoene synthase;
prephytoene-diphosphate synthase (ambiguous);
phytoene synthetase (ambiguous);
PSase (ambiguous);
geranylgeranyl-diphosphate geranylgeranyltransferase (ambiguous);
15-trans-phytoene synthase
Class
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
BRITE hierarchy
Sysname
geranylgeranyl-diphosphate:geranylgeranyl-diphosphate geranylgeranyltransferase (all-trans-phytoene forming)
Reaction(IUBMB)
2 geranylgeranyl diphosphate = all-trans-phytoene + 2 diphosphate (overall reaction) [RN:R07916];
(1a) 2 geranylgeranyl diphosphate = diphosphate + prephytoene diphosphate [RN:R02065];
(1b) prephytoene diphosphate = all-trans-phytoene + diphosphate [RN:R07270]
Reaction(KEGG)
Substrate
geranylgeranyl diphosphate [CPD:C00353];
prephytoene diphosphate [CPD:C03427]
Product
all-trans-phytoene [CPD:C05413];
diphosphate [CPD:C00013];
prephytoene diphosphate [CPD:C03427]
Comment
Requires Mn2+ for activity. The enzyme from the bacterium Pantoea agglomerans (previously known as Erwinia herbicola) produces the all-trans isomer of phytoene. cf. EC 2.5.1.32, 15-cis-phytoene synthase.
History
EC 2.5.1.99 created 2012
Reference
1  [PMID:4819767]
  Authors
Gregonis DE, Rilling HC.
  Title
The stereochemistry of trans-phytoene synthesis. Some observations on lycopersene as a carotene precursor and a mechanism for the synthesis of cis- and trans-phytoene.
  Journal
Biochemistry. 13 (1974) 1538-42.
Reference
2  [PMID:12641468]
  Authors
Iwata-Reuyl D, Math SK, Desai SB, Poulter CD.
  Title
Bacterial phytoene synthase: molecular cloning, expression, and characterization of Erwinia herbicola phytoene synthase.
  Journal
Biochemistry. 42 (2003) 3359-65.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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