KEGG   ENZYME: 2.6.1.93Help
Entry
EC 2.6.1.93                 Enzyme                                 

Name
neamine transaminase;
glutamate---6'-dehydroparomamine aminotransferase;
btrB (gene name);
neoN (gene name);
kacL (gene name)
Class
Transferases;
Transferring nitrogenous groups;
Transaminases
BRITE hierarchy
Sysname
neamine:2-oxoglutarate aminotransferase
Reaction(IUBMB)
neamine + 2-oxoglutarate = 6'-dehydroparomamine + L-glutamate [RN:R08897]
Reaction(KEGG)
Substrate
neamine [CPD:C01441];
2-oxoglutarate [CPD:C00026]
Product
6'-dehydroparomamine [CPD:C17583];
L-glutamate [CPD:C00025]
Comment
The reaction occurs in vivo in the opposite direction. Involved in the biosynthetic pathways of several clinically important aminocyclitol antibiotics, including kanamycin B, butirosin, neomycin and ribostamycin. Works in combination with EC 1.1.3.43, paromamine 6-oxidase, to replace the 6'-hydroxy group of paromamine with an amino group. The enzyme from the bacterium Streptomyces kanamyceticus can also catalyse EC 2.6.1.94, 2'-deamino-2'-hydroxyneamine transaminase, which leads to production of kanamycin A [3]. The enzyme from the bacterium Streptomyces fradiae can also catalyse EC 2.6.1.95, leading to production of neomycin C [2].
Pathway
Butirosin and neomycin biosynthesis
Biosynthesis of secondary metabolites
Orthology
K13553  
neamine transaminase / 2'-deamino-2'-hydroxyneamine transaminase / neomycin C transaminase
Reference
1  [PMID:17206729]
  Authors
Huang F, Spiteller D, Koorbanally NA, Li Y, Llewellyn NM, Spencer JB.
  Title
Elaboration of neosamine rings in the biosynthesis of neomycin and butirosin.
  Journal
Chembiochem. 8 (2007) 283-8.
  Organism
Bacillus circulans, Streptomyces fradiae
  Sequence
[up:Q4H4F5]
Reference
2  [PMID:21689223]
  Authors
Clausnitzer D, Piepersberg W, Wehmeier UF
  Title
The oxidoreductases LivQ and NeoQ are responsible for the different 6'-modifications in the aminoglycosides lividomycin and neomycin.
  Journal
J. Appl. Microbiol. 111 (2011) 642-51.
  Organism
Streptomyces fradiae
  Sequence
[up:Q53U08]
Reference
3  [PMID:21983602]
  Authors
Park JW, Park SR, Nepal KK, Han AR, Ban YH, Yoo YJ, Kim EJ, Kim EM, Kim D, Sohng JK, Yoon YJ
  Title
Discovery of parallel pathways of kanamycin biosynthesis allows antibiotic manipulation.
  Journal
Nat. Chem. Biol. 7 (2011) 843-52.
  Organism
Streptomyces kanamyceticus
  Sequence
[up:Q6L741]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

DBGET integrated database retrieval system