KEGG   ENZYME: 2.6.1.95Help
Entry
EC 2.6.1.95                 Enzyme                                 

Name
neomycin C transaminase;
neoN (gene name)
Class
Transferases;
Transferring nitrogenous groups;
Transaminases
BRITE hierarchy
Sysname
2-oxoglutarate:neomycin C aminotransferase
Reaction(IUBMB)
neomycin C + 2-oxoglutarate = 6'''-deamino-6'''-oxoneomycin C + L-glutamate [RN:R08903]
Reaction(KEGG)
Substrate
neomycin C [CPD:C15652];
2-oxoglutarate [CPD:C00026]
Product
6'''-deamino-6'''-oxoneomycin C [CPD:C17589];
L-glutamate [CPD:C00025]
Comment
The reaction occurs in vivo in the opposite direction. Involved in the biosynthetic pathway of aminoglycoside antibiotics of the neomycin family. Works in combination with EC 1.1.3.44, 6'''-hydroxyneomycin C oxidase, to replace the 6'''-hydroxy group of 6'''-deamino-6'''-hydroxyneomycin C with an amino group.
The enzyme, characterized from the bacterium Streptomyces fradiae, can also catalyse EC 2.6.1.93, neamine transaminase.
History
EC 2.6.1.95 created 2012
Pathway
Butirosin and neomycin biosynthesis
Orthology
K13553  
neamine transaminase / 2'-deamino-2'-hydroxyneamine transaminase / neomycin C transaminase
Reference
1  [PMID:17206729]
  Authors
Huang F, Spiteller D, Koorbanally NA, Li Y, Llewellyn NM, Spencer JB.
  Title
Elaboration of neosamine rings in the biosynthesis of neomycin and butirosin.
  Journal
Chembiochem. 8 (2007) 283-8.
Reference
2  [PMID:21689223]
  Authors
Clausnitzer D, Piepersberg W, Wehmeier UF
  Title
The oxidoreductases LivQ and NeoQ are responsible for the different 6'-modifications in the aminoglycosides lividomycin and neomycin.
  Journal
J. Appl. Microbiol. 111 (2011) 642-51.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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