KEGG   ENZYME: 2.7.1.160Help
Entry
EC 2.7.1.160                Enzyme                                 

Name
2'-phosphotransferase;
yeast 2'-phosphotransferase;
Tpt1;
Tpt1p;
2'-phospho-tRNA:NAD+ phosphotransferase
Class
Transferases;
Transferring phosphorus-containing groups;
Phosphotransferases with an alcohol group as acceptor
BRITE hierarchy
Sysname
2'-phospho-[ligated tRNA]:NAD+ phosphotransferase
Reaction(IUBMB)
2'-phospho-[ligated tRNA] + NAD+ = mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide
Substrate
2'-phospho-[ligated tRNA];
NAD+ [CPD:C00003]
Product
mature tRNA;
ADP-ribose 1'',2''-phosphate [CPD:C19851];
nicotinamide [CPD:C00153]
Comment
Catalyses the final step of tRNA splicing in the yeast Saccharomyces cerevisiae [2]. The reaction takes place in two steps: in the first step, the 2'-phosphate on the RNA substrate is ADP-ribosylated, causing the relase of nicotinamide and the formation of the reaction intermediate, ADP-ribosylated tRNA [6]. In the second step, dephosphorylated (mature) tRNA is formed along with ADP ribose 1''-2''-cyclic phosphate. Highly specific for oligonucleotide substrates bearing an internal 2'-phosphate. Oligonucleotides with only a terminal 5'- or 3'-phosphate are not substrates [1].
History
EC 2.7.1.160 created 2006
Orthology
K10669  
2'-phosphotransferase
Genes
HSA: 
83707(TRPT1)
PTR: 
451288(TRPT1)
PPS: 
100985392(TRPT1)
GGO: 
101147538(TRPT1)
PON: 
100457497(TRPT1)
NLE: 
100598276(TRPT1)
MCC: 
717911(TRPT1)
MCF: 
102130373(TRPT1)
CSAB: 
103233411(TRPT1)
RRO: 
104673027(TRPT1)
CJC: 
100410369(TRPT1)
SBQ: 
101047098(TRPT1)
MMU: 
107328(Trpt1)
RNO: 
293704(Trpt1)
CGE: 
100756285(Trpt1)
NGI: 
103738804(Trpt1)
HGL: 
101701513(Trpt1)
OCU: 
100356527(TRPT1)
TUP: 
102492696(TRPT1)
CFA: 
483765(TRPT1)
AML: 
100474877(TRPT1)
UMR: 
103679419(TRPT1)
FCA: 
101091731(TRPT1) 105259622(TRPT1)
PTG: 
102962279(TRPT1)
BTA: 
510729(TRPT1)
BOM: 
102277874(TRPT1)
PHD: 
102328322(TRPT1)
CHX: 
102179356(TRPT1)
OAS: 
101122992(TRPT1)
SSC: 
100521427(TRPT1)
CFR: 
102521618(TRPT1)
BACU: 
103009681(TRPT1)
LVE: 
103086666(TRPT1)
ECB: 
100055143(TRPT1)
MYB: 
102261357(TRPT1)
MYD: 
102773847(TRPT1)
PALE: 
102880757(TRPT1)
LAV: 
100669704(TRPT1)
MDO: 
100028474(TRPT1)
SHR: 
100921638(TRPT1)
OAA: 
100079825(TRPT1)
GGA: 
107049849(TRPT1)
MGP: 
104915994(TRPT1)
CJO: 
107307908(TRPT1)
ASN: 
102373360(TRPT1)
AMJ: 
102559246(TRPT1)
PSS: 
102447359(TRPT1)
CMY: 
102932919(TRPT1)
ACS: 
100564547(trpt1)
PBI: 
103063200(TRPT1)
GJA: 
107115198(TRPT1)
XTR: 
100216285(trpt1)
DRE: 
503604(trpt1)
TRU: 
101079304(trpt1)
TNG: 
MZE: 
101469051(trpt1)
OLA: 
101156179(trpt1)
XMA: 
102222191(trpt1)
SASA: 
LCM: 
102351021(TRPT1)
BFO: 
CIN: 
SPU: 
SKO: 
DME: 
DPO: 
DSE: 
DSI: 
Dsimw501_GD12992(Dsim_GD12992)
DYA: 
Dyak_GE21308(dyak_GLEANR_5078)
DGR: 
DMO: 
DVI: 
MDE: 
SOC: 
AEC: 
HST: 
CFO: 
NVI: 
TCA: 
PXY: 
HRO: 
LGI: 
CRG: 
OBI: 
SMM: 
NVE: 
ADF: 
AQU: 
ATH: 
ALY: 
CRB: 
EUS: 
BRP: 
BNA: 
THJ: 
CIT: 
CIC: 
TCC: 
GRA: 
EGR: 
GMX: 
PVU: 
VRA: 
MTR: 
CAM: 
ADU: 
AIP: 
LJA: 
Lj3g3v2735060.1(Lj3g3v2735060.1)
FVE: 
PPER: 
PMUM: 
MDM: 
PXB: 
CSV: 
CMO: 
RCU: 
JCU: 
POP: 
POPTR_0002s11780g(POPTRDRAFT_1071929) POPTR_0013s09110g(POPTRDRAFT_663425)
VVI: 
SLY: 
SPEN: 
SOT: 
SIND: 
BVG: 
NNU: 
OSA: 
9267091(P0001H02.6)
DOSA: 
Os06t0134050-01(Os06g0134050)
OBR: 
BDI: 
ATS: 
SBI: 
SORBI_10g002260(SORBIDRAFT_10g002260)
ZMA: 
100273223(GRMZM2G061783) 103638121(GRMZM2G092568)
SITA: 
PDA: 
EGU: 
MUS: 
ATR: 
SMO: 
PPP: 
MIS: 
MPP: 
CVR: 
SCE: 
YOL102C(TPT1)
AGO: 
ERC: 
KLA: 
LTH: 
VPO: 
ZRO: 
CGR: 
NCS: 
NCAS_0C04770(NCAS0C04770)
NDI: 
NDAI_0G04120(NDAI0G04120)
TPF: 
TPHA_0P01070(TPHA0P01070)
TBL: 
TBLA_0B08270(TBLA0B08270)
TDL: 
TDEL_0D05360(TDEL0D05360)
KAF: 
KAFR_0C01470(KAFR0C01470)
PPA: 
DHA: 
PIC: 
PGU: 
SPAA: 
LEL: 
CAL: 
CTP: 
COT: 
CDU: 
CTEN: 
YLI: 
CLU: 
NCR: 
NTE: 
NEUTE1DRAFT62747(NEUTE1DRAFT_62747)
SMP: 
PAN: 
TTT: 
MTM: 
CTHR: 
MGR: 
TMN: 
FGR: 
FPU: 
NHE: 
TRE: 
MAW: 
MAJ: 
CMT: 
VAL: 
VDA: 
ELA: 
SSL: 
BFU: 
MBE: 
AFM: 
AFV: 
ACT: 
NFI: 
CIM: 
CIMG_11231(CIMG03943)
PBL: 
PBN: 
PADG_11258(PADG_01445)
ABE: 
TVE: 
PNO: 
PTE: 
BZE: 
BSC: 
BOR: 
ZTR: 
PFJ: 
BCOM: 
SPO: 
CGI: 
TMS: 
PPL: 
DSQ: 
PCO: 
SHS: 
HIR: 
PSQ: 
ADL: 
FME: 
GTR: 
LBC: 
MRR: 
CCI: 
SCM: 
ABP: 
AGABI1DRAFT100698(AGABI1DRAFT_100698) AGABI1DRAFT70670(AGABI1DRAFT_70670)
ABV: 
AGABI2DRAFT149451(AGABI2DRAFT_149451) AGABI2DRAFT206430(AGABI2DRAFT_206430)
CPUT: 
SLA: 
WSE: 
WIC: 
UMA: 
PFP: 
PGR: 
MLR: 
DDI: 
DPP: 
DFA: 
EDI: 
EIV: 
ACAN: 
TGO: 
TET: 
PTM: 
SMIN: 
v1.2.004831.t1(symbB.v1.2.004831.t1) v1.2.018195.t3(symbB.v1.2.018195.t3) v1.2.023881.t1(symbB.v1.2.023881.t1) v1.2.032499.t1(symbB.v1.2.032499.t1)
PTI: 
PSOJ: 
SPAR: 
EHX: 
GTT: 
TBR: 
TCR: 
LMA: 
NGR: 
 » show all
Taxonomy
Reference
1  [PMID:11705403]
  Authors
Steiger MA, Kierzek R, Turner DH, Phizicky EM.
  Title
Substrate recognition by a yeast 2'-phosphotransferase involved in tRNA splicing and by its Escherichia coli homolog.
  Journal
Biochemistry. 40 (2001) 14098-105.
Reference
2  [PMID:9915792]
  Authors
Spinelli SL, Kierzek R, Turner DH, Phizicky EM.
  Title
Transient ADP-ribosylation of a 2'-phosphate implicated in its removal from ligated tRNA during splicing in yeast.
  Journal
J. Biol. Chem. 274 (1999) 2637-44.
  Sequence
[sce:YOL102C]
Reference
3  [PMID:9148937]
  Authors
Culver GM, McCraith SM, Consaul SA, Stanford DR, Phizicky EM.
  Title
A 2'-phosphotransferase implicated in tRNA splicing is essential in Saccharomyces cerevisiae.
  Journal
J. Biol. Chem. 272 (1997) 13203-10.
  Sequence
[sce:YOL102C]
Reference
4  [PMID:2050693]
  Authors
McCraith SM, Phizicky EM.
  Title
An enzyme from Saccharomyces cerevisiae uses NAD+ to transfer the splice junction 2'-phosphate from ligated tRNA to an acceptor molecule.
  Journal
J. Biol. Chem. 266 (1991) 11986-92.
Reference
5  [PMID:14504659]
  Authors
Hu QD, Lu H, Huo K, Ying K, Li J, Xie Y, Mao Y, Li YY.
  Title
A human homolog of the yeast gene encoding tRNA 2'-phosphotransferase: cloning, characterization and complementation analysis.
  Journal
Cell. Mol. Life. Sci. 60 (2003) 1725-32.
  Sequence
[hsa:83707]
Reference
6  [PMID:15611300]
  Authors
Steiger MA, Jackman JE, Phizicky EM.
  Title
Analysis of 2'-phosphotransferase (Tpt1p) from Saccharomyces cerevisiae: evidence for a conserved two-step reaction mechanism.
  Journal
RNA. 11 (2005) 99-106.
Reference
7  [PMID:15611301]
  Authors
Sawaya R, Schwer B, Shuman S.
  Title
Structure-function analysis of the yeast NAD+-dependent tRNA 2'-phosphotransferase Tpt1.
  Journal
RNA. 11 (2005) 107-13.
Reference
8  [PMID:15811369]
  Authors
Kato-Murayama M, Bessho Y, Shirouzu M, Yokoyama S.
  Title
Crystal structure of the RNA 2'-phosphotransferase from Aeropyrum pernix K1.
  Journal
J. Mol. Biol. 348 (2005) 295-305.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
126905-00-8

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