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Entry
EC 2.7.7.69                 Enzyme                                 

Name
GDP-L-galactose phosphorylase;
VTC2;
VTC5
Class
Transferases;
Transferring phosphorus-containing groups;
Nucleotidyltransferases
BRITE hierarchy
Sysname
GDP:alpha-L-galactose 1-phosphate guanylyltransferase
Reaction(IUBMB)
GDP-beta-L-galactose + phosphate = beta-L-galactose 1-phosphate + GDP
Reaction(KEGG)
(other) R07678
Show
Substrate
GDP-beta-L-galactose;
phosphate [CPD:C00009]
Product
beta-L-galactose 1-phosphate [CPD:C15926];
GDP [CPD:C00035]
Comment
The enzyme catalyses a reaction of the Smirnoff-Wheeler pathway, the major route to ascorbate biosynthesis in plants.
History
EC 2.7.7.69 created 2010
Pathway
Ascorbate and aldarate metabolism
Metabolic pathways
Biosynthesis of secondary metabolites
Orthology
K14190  
GDP-L-galactose phosphorylase
Genes
ATH: 
AT4G26850(VTC2) AT5G55120(VTC5)
ALY: 
CRB: 
EUS: 
CIT: 
CIC: 
TCC: 
GMX: 
PVU: 
MTR: 
CAM: 
FVE: 
PPER: 
CSV: 
RCU: 
POP: 
POPTR_0001s36560g(POPTRDRAFT_642193) POPTR_0005s14440g POPTR_0011s08940g(POPTRDRAFT_660608)
VVI: 
SLY: 
SOT: 
OSA: 
DOSA: 
Os12t0190000-01(Os12g0190000)
OBR: 
BDI: 
SBI: 
SORBI_08g005410(SORBIDRAFT_08g005410)
ZMA: 
100272546(si946084h12) 100283852
SITA: 
ATR: 
s00007p00255940(AMTR_s00007p00255940) s00155p00050480(AMTR_s00155p00050480)
SMO: 
PPP: 
CRE: 
VCN: 
OLU: 
MIS: 
MPP: 
BPG: 
CSL: 
CVR: 
 » show all
Taxonomy
Reference
1  [PMID:17462988]
  Authors
Linster CL, Gomez TA, Christensen KC, Adler LN, Young BD, Brenner C, Clarke SG.
  Title
Arabidopsis VTC2 Encodes a GDP-L-Galactose Phosphorylase, the Last Unknown Enzyme in the Smirnoff-Wheeler Pathway to Ascorbic Acid in Plants.
  Journal
J. Biol. Chem. 282 (2007) 18879-85.
  Organism
Arabidopsis thaliana
  Sequence
[ath:AT4G26850]
Reference
2  [PMID:18463094]
  Authors
Linster CL, Adler LN, Webb K, Christensen KC, Brenner C, Clarke SG
  Title
A second GDP-L-galactose phosphorylase in arabidopsis en route to vitamin C. Covalent intermediate and substrate requirements for the conserved reaction.
  Journal
J. Biol. Chem. 283 (2008) 18483-92.
  Organism
Arabidopsis thaliana
  Sequence
Reference
3  [PMID:17877701]
  Authors
Dowdle J, Ishikawa T, Gatzek S, Rolinski S, Smirnoff N
  Title
Two genes in Arabidopsis thaliana encoding GDP-L-galactose phosphorylase are required for ascorbate biosynthesis and seedling viability.
  Journal
Plant. J. 52 (2007) 673-89.
  Organism
Arabidopsis thaliana
  Sequence
Reference
4  [PMID:18516687]
  Authors
Muller-Moule P
  Title
An expression analysis of the ascorbate biosynthesis enzyme VTC2.
  Journal
Plant. Mol. Biol. 68 (2008) 31-41.
  Organism
Arabidopsis thaliana
  Sequence
[ath:AT4G26850]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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