KEGG   ENZYME: 2.7.99.1Help
Entry
EC 2.7.99.1                 Enzyme                                 

Name
triphosphate---protein phosphotransferase;
diphosphate:microsomal-membrane-protein O-phosphotransferase (erroneous);
DiPPT (erroneous);
pyrophosphate:protein phosphotransferase (erroneous);
diphosphate---protein phosphotransferase (erroneous);
diphosphate:[microsomal-membrane-protein] O-phosphotransferase (erroneous)
Class
Transferases;
Transferring phosphorus-containing groups;
Other protein kinases
BRITE hierarchy
Sysname
triphosphate:[microsomal-membrane-protein] phosphotransferase
Reaction(IUBMB)
triphosphate + [microsomal-membrane protein] = diphosphate + phospho-[microsomal-membrane protein] [RN:R04312]
Reaction(KEGG)
Substrate
triphosphate [CPD:C00536];
microsomal-membrane protein [CPD:C03788]
Product
diphosphate [CPD:C00013];
phospho-[microsomal-membrane protein]
Comment
This enzyme was originally thought to use diphosphate as substrate [1] but this has since been disproved [2]. The activity is observed as the second part of a biphasic reaction after depletion of ATP. Tripolyphosphate is a contaminant of [gamma-32P]ATP.
History
EC 2.7.99.1 created 1983 as EC 2.7.1.104, transferred 2005 to EC 2.7.99.1
Reference
1  [PMID:6246514]
  Authors
Lam KS, Kasper CB.
  Title
Pyrophosphate:protein phosphotransferase: a membrane-bound enzyme of endoplasmic reticulum.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 77 (1980) 1927-31.
Reference
2  [PMID:3949741]
  Authors
Tsutsui K.
  Title
Tripolyphosphate is an alternative phosphodonor of the selective protein phosphorylation of liver microsomal membrane.
  Journal
J. Biol. Chem. 261 (1986) 2645-53.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
74092-32-3

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