KEGG   ENZYME: 2.8.1.11Help
Entry
EC 2.8.1.11                 Enzyme                                 

Name
molybdopterin synthase sulfurtransferase;
adenylyltransferase and sulfurtransferase MOCS3;
Cnx5 (gene name);
molybdopterin synthase sulfurylase
Class
Transferases;
Transferring sulfur-containing groups;
Sulfurtransferases
BRITE hierarchy
Sysname
persulfurated L-cysteine desulfurase:[molybdopterin-synthase sulfur-carrier protein]-Gly-Gly sulfurtransferase
Reaction(IUBMB)
[molybdopterin-synthase sulfur-carrier protein]-Gly-Gly-AMP + [cysteine desulfurase]-S-sulfanyl-L-cysteine = AMP + [molybdopterin-synthase sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + cysteine desulfurase
Substrate
[molybdopterin-synthase sulfur-carrier protein]-Gly-Gly-AMP;
[cysteine desulfurase]-S-sulfanyl-L-cysteine
Product
AMP [CPD:C00020];
[molybdopterin-synthase sulfur-carrier protein]-Gly-NH-CH2-C(O)SH;
cysteine desulfurase
Comment
The enzyme transfers sulfur to form a thiocarboxylate moiety on the C-terminal glycine of the small subunit of EC 2.8.1.12, molybdopterin synthase. In the human, the reaction is catalysed by the rhodanese-like C-terminal domain (cf. EC 2.8.1.1) of the MOCS3 protein, a bifunctional protein that also contains EC 2.7.7.80, molybdopterin-synthase adenylyltransferase, at the N-terminal domain.
History
EC 2.8.1.11 created 2011
Reference
1  [PMID:15910006]
  Authors
Matthies A, Nimtz M, Leimkuhler S
  Title
Molybdenum cofactor biosynthesis in humans: identification of a persulfide group  in the rhodanese-like domain of MOCS3 by mass spectrometry.
  Journal
Biochemistry. 44 (2005) 7912-20.
  Sequence
[hsa:27304]
Reference
2  [PMID:11290749]
  Authors
Leimkuhler S, Rajagopalan KV
  Title
A sulfurtransferase is required in the transfer of cysteine sulfur in the in vitro synthesis of molybdopterin from precursor Z in Escherichia coli.
  Journal
J. Biol. Chem. 276 (2001) 22024-31.
  Sequence
[eco:b0826]
Reference
3  [PMID:19798741]
  Authors
Hanzelmann P, Dahl JU, Kuper J, Urban A, Muller-Theissen U, Leimkuhler S, Schindelin H
  Title
Crystal structure of YnjE from Escherichia coli, a sulfurtransferase with three rhodanese domains.
  Journal
Protein. Sci. 18 (2009) 2480-91.
Reference
4  [PMID:21856748]
  Authors
Dahl JU, Urban A, Bolte A, Sriyabhaya P, Donahue JL, Nimtz M, Larson TJ, Leimkuhler S
  Title
The identification of a novel protein involved in molybdenum cofactor biosynthesis in Escherichia coli.
  Journal
J. Biol. Chem. 286 (2011) 35801-12.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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