KEGG   ENZYME: 2.8.2.31Help
Entry
EC 2.8.2.31                 Enzyme                                 

Name
petromyzonol sulfotransferase;
PZ-SULT
Class
Transferases;
Transferring sulfur-containing groups;
Sulfotransferases
BRITE hierarchy
Sysname
3'-phosphoadenylyl-sulfate:5alpha-cholan-3alpha,7alpha,12alpha,24-tetrol sulfotransferase
Reaction(IUBMB)
3'-phosphoadenylyl sulfate + 5alpha-cholan-3alpha,7alpha,12alpha,24-tetrol = adenosine 3',5'-bisphosphate + 5alpha-cholan-3alpha,7alpha,12alpha-triol 24-sulfate [RN:R07797]
Reaction(KEGG)
Substrate
3'-phosphoadenylyl sulfate [CPD:C00053];
5alpha-cholan-3alpha,7alpha,12alpha,24-tetrol [CPD:C16258]
Product
adenosine 3',5'-bisphosphate [CPD:C00054];
5alpha-cholan-3alpha,7alpha,12alpha-triol 24-sulfate [CPD:C16259]
Comment
The enzyme from the lamprey Petromyzon marinus can also use the corresponding 3-ketone as a substrate. It is stereoselective (5alpha-cholane) and regioselective, exhibiting a preference for an hydroxy group at C-24. The enzyme is inactive when allocholic acid, which has a carboxy group at C-24, is used as a substrate.
History
EC 2.8.2.31 created 2004
Reference
1  [PMID:14657197]
  Authors
Venkatachalam KV, Llanos DE, Karami KJ, Malinovskii VA.
  Title
Isolation, partial purification, and characterization of a novel petromyzonol sulfotransferase from Petromyzon marinus (lamprey) larval liver.
  Journal
J. Lipid. Res. 45 (2004) 486-95.
  Organism
Petromyzon marinus
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

DBGET integrated database retrieval system