KEGG   ENZYME: 2.8.2.33Help
Entry
EC 2.8.2.33                 Enzyme                                 

Name
N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase;
GalNAc4S-6ST;
CHST15 (gene name)
Class
Transferases;
Transferring sulfur-containing groups;
Sulfotransferases
BRITE hierarchy
Sysname
3'-phosphoadenylyl-sulfate:[dermatan]-4-O-sulfo-N-acetyl-D-galactosamine 6-O-sulfotransferase
Reaction(IUBMB)
(1) 3'-phospho-5'-adenylyl sulfate + [dermatan]-4-O-sulfo-N-acetyl-D-galactosamine = adenosine 3',5'-bisphosphate + [dermatan]-4,6-di-O-sulfo-N-acetyl-D-galactosamine [RN:R10868];
(2) 3'-phospho-5'-adenylyl sulfate + [chondroitin]-4-O-sulfo-N-acetyl-D-galactosamine = adenosine 3',5'-bisphosphate + [chondroitin]-4,6-di-O-sulfo-N-acetyl-D-galactosamine [RN:R10869]
Reaction(KEGG)
Substrate
3'-phospho-5'-adenylyl sulfate [CPD:C00053];
[dermatan]-4-O-sulfo-N-acetyl-D-galactosamine;
[chondroitin]-4-O-sulfo-N-acetyl-D-galactosamine
Product
adenosine 3',5'-bisphosphate [CPD:C00054];
[dermatan]-4,6-di-O-sulfo-N-acetyl-D-galactosamine;
[chondroitin]-4,6-di-O-sulfo-N-acetyl-D-galactosamine
Comment
The enzyme is activated by divalent cations and reduced glutathione. The enzyme from human transfers sulfate to position 6 of both internal residues and non-reducing terminal GalNAc 4-sulfate residues of chondroitin sulfate and dermatan sulfate. Oligosaccharides derived from chondroitin sulfate also serve as acceptors but chondroitin sulfate E, keratan sulfate and heparan sulfate do not. Differs from EC 2.8.2.17, chondroitin 6-sulfotransferase, in being able to use both chondroitin and dermatan as effective substrates
History
EC 2.8.2.33 created 2005, modified 2010
Pathway
ec00532  Glycosaminoglycan biosynthesis - chondroitin sulfate / dermatan sulfate
Orthology
K08106  N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase
Genes
HSA: 51363(CHST15)
PTR: 466249(CHST15)
PPS: 100988573(CHST15)
GGO: 101139112(CHST15)
PON: 100446124(CHST15)
NLE: 100593528(CHST15)
MCC: 693845(CHST15)
MCF: 102140207(CHST15)
CSAB: 103216659(CHST15)
RRO: 104657648(CHST15)
RBB: 108519585(CHST15)
CJC: 100400214(CHST15)
SBQ: 101052289(CHST15)
MMU: 77590(Chst15)
RNO: 286974(Chst15)
CGE: 100768085(Chst15)
NGI: 103729255(Chst15)
HGL: 101726691(Chst15)
CCAN: 109691645(Chst15)
OCU: 100343176(CHST15)
TUP: 102470510(CHST15)
CFA: 486928(CHST15)
AML: 100472799(CHST15)
UMR: 103658000(CHST15)
ORO: 101386186(CHST15)
FCA: 101098166(CHST15)
PTG: 102959008(CHST15)
AJU: 106971732(CHST15)
BTA: 534506(CHST15)
BOM: 102270502(CHST15)
BIU: 109553118(CHST15)
PHD: 102326413(CHST15)
CHX: 102179262(CHST15)
OAS: 101113976(CHST15)
SSC: 100152393(CHST15)
CFR: 102518619(CHST15)
CDK: 105100278(CHST15)
BACU: 103000709(CHST15)
LVE: 103088154(CHST15)
OOR: 101287863(CHST15)
ECB: 100057594(CHST15)
EPZ: 103548446(CHST15)
EAI: 106847753(CHST15)
MYB: 102256050(CHST15)
MYD: 102768574(CHST15)
HAI: 109396924(CHST15)
RSS: 109452900(CHST15)
PALE: 102882217(CHST15)
LAV: 100659572(CHST15)
TMU: 101354169
MDO: 100025063(CHST15)
SHR: 100913832 100926169(CHST15)
OAA: 100087988(CHST15)
GGA: 423952(CHST15)
MGP: 100542904(CHST15)
CJO: 107316272(CHST15)
APLA: 101792968(CHST15)
ACYG: 106038980(CHST15)
TGU: 100221434(CHST15)
GFR: 102041118(CHST15)
FAB: 101819890(CHST15)
PHI: 102110600(CHST15)
PMAJ: 107207093(CHST15)
CCW: 104694538(CHST15)
FPG: 101915195(CHST15)
FCH: 102046885(CHST15)
CLV: 102094121(CHST15)
EGZ: 104128314(CHST15)
AAM: 106494366(CHST15)
ASN: 102381919(CHST15)
AMJ: 102565249(CHST15)
PSS: 102454589(CHST15)
CMY: 102936402(CHST15)
CPIC: 101935858(CHST15)
ACS: 100557550(chst15)
PVT: 110070375(CHST15)
PBI: 103065969(CHST15)
GJA: 107123681(CHST15)
XLA: 108696098(chst15.L) 108697373
XTR: 100487411(chst15)
NPR: 108784782(CHST15)
DRE: 565523(chst15)
IPU: 108273603(chst15)
TRU: 101062081 101075284(chst15)
LCO: 104925926(chst15) 104929338
OLA: 101167818 101174629(chst15)
XMA: 102220050(chst15) 102227019
PRET: 103476751 103481828(chst15)
NFU: 107375978 107387792(chst15)
LCF: 108899055(chst15)
HCQ: 109510229 109513397(chst15)
BPEC: 110165469(chst15)
ELS: 105009432(chst15)
SFM: 108932796 108939475(chst15)
LCM: 102359407(CHST15)
CMK: 103185442(chst15)
SPAR: SPRG_01829
 » show all
Taxonomy
Reference
1  [PMID:10871629]
  Authors
Ito Y, Habuchi O.
  Title
Purification and characterization of N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase from the squid cartilage.
  Journal
J. Biol. Chem. 275 (2000) 34728-36.
  Sequence
Reference
2  [PMID:11572857]
  Authors
Ohtake S, Ito Y, Fukuta M, Habuchi O.
  Title
Human N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase cDNA is related to human B cell recombination activating gene-associated gene.
  Journal
J. Biol. Chem. 276 (2001) 43894-900.
  Sequence
[hsa:51363] [ag:BAB72146]
Other DBs
ExplorEnz - The Enzyme Database: 2.8.2.33
IUBMB Enzyme Nomenclature: 2.8.2.33
ExPASy - ENZYME nomenclature database: 2.8.2.33
BRENDA, the Enzyme Database: 2.8.2.33
CAS: 242469-38-1

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