KEGG   ENZYME: 2.8.3.15Help
Entry
EC 2.8.3.15                 Enzyme                                 

Name
succinyl-CoA:(R)-benzylsuccinate CoA-transferase;
benzylsuccinate CoA-transferase
Class
Transferases;
Transferring sulfur-containing groups;
CoA-transferases
BRITE hierarchy
Sysname
succinyl-CoA:(R)-2-benzylsuccinate CoA-transferase
Reaction(IUBMB)
succinyl-CoA + (R)-2-benzylsuccinate = succinate + (R)-2-benzylsuccinyl-CoA [RN:R05588]
Reaction(KEGG)
Substrate
succinyl-CoA [CPD:C00091];
(R)-2-benzylsuccinate [CPD:C09816]
Product
succinate [CPD:C00042];
(R)-2-benzylsuccinyl-CoA [CPD:C09817]
Comment
Involved in anaerobic catabolism of toluene and is a strictly toluene-induced enzyme that catalyses the reversible regio- and enantio-selective synthesis of (R)-2-benzylsuccinyl-CoA. The enzyme from Thauera aromatica is inactive when (R)-benzylsuccinate is replaced by (S)-benzylsuccinate.
History
EC 2.8.3.15 created 2003
Pathway
Toluene degradation
Metabolic pathways
Microbial metabolism in diverse environments
Orthology
K07543  
benzylsuccinate CoA-transferase BbsE subunit
K07544  
benzylsuccinate CoA-transferase BbsF subunit
Genes
BYI: 
BUE: 
BBR: 
BB4333(bbsE) BB4334(bbsF)
BBX: 
AXY: 
VEI: 
EBA: 
c2A311(bbsF) c2A312(bbsE)
AZI: 
GME: 
Gmet_1521(bbsE) Gmet_1522(bbsF)
GEO: 
Geob_2420(bbsE) Geob_2421(bbsF)
DTO: 
AMQ: 
 » show all
Taxonomy
Reference
1  [PMID:11418570]
  Authors
Leutwein C, Heider J.
  Title
Succinyl-CoA:(R)-benzylsuccinate CoA-transferase: an enzyme of the anaerobic toluene catabolic pathway in denitrifying bacteria.
  Journal
J. Bacteriol. 183 (2001) 4288-95.
  Sequence
Reference
2  [PMID:10589736]
  Authors
Leutwein C, Heider J.
  Title
Anaerobic toluene-catabolic pathway in denitrifying Thauera aromatica: activation and beta-oxidation of the first intermediate, (R)-(+)-benzylsuccinate.
  Journal
Microbiology. 145 ( Pt 11) (1999) 3265-71.
Reference
3  [PMID:10629170]
  Authors
Leuthner B, Heider J.
  Title
Anaerobic toluene catabolism of Thauera aromatica: the bbs operon codes for enzymes of beta oxidation of the intermediate benzylsuccinate.
  Journal
J. Bacteriol. 182 (2000) 272-7.
  Sequence
Reference
4  [PMID:11749953]
  Authors
Heider J.
  Title
A new family of CoA-transferases.
  Journal
FEBS. Lett. 509 (2001) 345-9.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
UM-BBD (Biocatalysis/Biodegradation Database): 
BRENDA, the Enzyme Database: 
CAS: 
260966-56-1

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