KEGG   ENZYME: 3.1.1.76Help
Entry
EC 3.1.1.76                 Enzyme                                 

Name
poly(3-hydroxyoctanoate) depolymerase;
PHO depolymerase;
poly(3HO) depolymerase;
poly[(R)-hydroxyalkanoic acid] depolymerase;
poly(HA) depolymerase;
poly(HAMCL) depolymerase;
poly[(R)-3-hydroxyoctanoate] hydrolase
Class
Hydrolases;
Acting on ester bonds;
Carboxylic-ester hydrolases
BRITE hierarchy
Sysname
poly{oxycarbonyl[(R)-2-pentylethylene]} hydrolase
Reaction(IUBMB)
Hydrolyses the polyester poly{oxycarbonyl[(R)-2-pentylethylene]} to oligomers [RN:R05796]
Reaction(KEGG)
Comment
The main product after prolonged incubation is the dimer [3]. Besides hydrolysing polymers of 3-hydroxyoctanoic acid, the enzyme also hydrolyses other polymers derived from medium-chain-length (C6-C12) hydroxyalkanoic acids and copolymers of mixtures of these. It also hydrolyses p-nitrophenyl esters of fatty acids. Polymers of short-chain-length hydroxyalkanoic acids such as poly[(R)-3-hydroxybutanoic acid] and poly[(R)-3-hydroxypentanoic acid] are not hydrolysed.
History
EC 3.1.1.76 created 2001, modified 2005
Reference
1  [PMID:11217416]
  Authors
Jendrossek D.
  Title
Microbial degradation of polyesters.
  Journal
Adv. Biochem. Eng. Biotechnol. 71 (2001) 293-325.
Reference
2  [PMID:10506180]
  Authors
Garcia B, Olivera ER, Minambres B, Fernandez-Valverde M, Canedo LM, Prieto MA, Garcia JL, Martinez M, Luengo JM.
  Title
Novel biodegradable aromatic plastics from a bacterial source. Genetic and biochemical studies on a route of the phenylacetyl-coa catabolon.
  Journal
J. Biol. Chem. 274 (1999) 29228-41.
Reference
3  [PMID:8476295]
  Authors
Schirmer A, Jendrossek D, Schlegel HG.
  Title
Degradation of poly(3-hydroxyoctanoic acid) [P(3HO)] by bacteria: purification and properties of a P(3HO) depolymerase from Pseudomonas fluorescens GK13.
  Journal
Appl. Environ. Microbiol. 59 (1993) 1220-7.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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