KEGG   ENZYME: 3.1.1.78Help
Entry
EC 3.1.1.78                 Enzyme                                 

Name
polyneuridine-aldehyde esterase;
polyneuridine aldehyde esterase;
PNAE
Class
Hydrolases;
Acting on ester bonds;
Carboxylic-ester hydrolases
BRITE hierarchy
Sysname
polyneuridine aldehyde hydrolase (decarboxylating)
Reaction(IUBMB)
polyneuridine aldehyde + H2O = 16-epivellosimine + CO2 + methanol [RN:R05825]
Reaction(KEGG)
Substrate
polyneuridine aldehyde [CPD:C11632];
H2O [CPD:C00001]
Product
16-epivellosimine [CPD:C11633];
CO2 [CPD:C00011];
methanol [CPD:C00132]
Comment
Following hydrolysis of this indole alkaloid ester the carboxylic acid decarboxylates spontaneously giving the sarpagan skeleton. The enzyme also acts on akuammidine aldehyde (the 16-epimer of polyneuridine aldehyde).
History
EC 3.1.1.78 created 2002
Pathway
Indole alkaloid biosynthesis
Biosynthesis of secondary metabolites
Orthology
K08233  
polyneuridine-aldehyde esterase
Genes
ATH: 
AT2G23600(ACL) AT2G23610(MES3) AT2G23620(MES1) AT4G37150(MES9)
ALY: 
CRB: 
EUS: 
BRP: 
BNA: 
THJ: 
CIT: 
CIC: 
TCC: 
GRA: 
EGR: 
GMX: 
PVU: 
VRA: 
MTR: 
CAM: 
ADU: 
AIP: 
LJA: 
Lj4g3v0843090.1(Lj4g3v0843090.1) Lj4g3v0843250.1(Lj4g3v0843250.1) Lj4g3v0853320.1(Lj4g3v0853320.1) Lj4g3v0853320.2(Lj4g3v0853320.2)
FVE: 
PPER: 
PMUM: 
MDM: 
PXB: 
CSV: 
CMO: 
RCU: 
JCU: 
POP: 
POPTR_0005s14030g POPTR_0005s14040g(POPTRDRAFT_558828) POPTR_0005s14050g POPTR_0005s14060g(POPTRDRAFT_558831) POPTR_0007s11650g(POPTRDRAFT_832353) POPTR_0007s11670g(POPTRDRAFT_764605) POPTR_0007s11680g(POPTRDRAFT_563033) POPTR_0007s11690g POPTR_0007s11700g POPTR_0007s11710g(POPTRDRAFT_563035) POPTR_0007s11720g(POPTRDRAFT_764610) POPTR_0007s11730g(POPTRDRAFT_764613) POPTR_0007s11750g(POPTRDRAFT_218861) POPTR_0011s02440g(POPTRDRAFT_568425)
VVI: 
SLY: 
SPEN: 
SOT: 
SIND: 
BVG: 
NNU: 
OSA: 
DOSA: 
Os01t0557100-01(Os01g0557100) Os01t0557200-00(Os01g0557200) Os01t0787600-01(Os01g0787600) Os01t0934600-00(Os01g0934600) Os01t0934700-01(Os01g0934700) Os01t0934800-00(Os01g0934800) Os01t0934900-00(Os01g0934900) Os11t0492800-00(Os11g0492800)
OBR: 
BDI: 
ATS: 
SBI: 
SORBI_03g024830(SORBIDRAFT_03g024830) SORBI_03g036770(SORBIDRAFT_03g036770) SORBI_03g045060(SORBIDRAFT_03g045060) SORBI_03g045070(SORBIDRAFT_03g045070) SORBI_03g045080(SORBIDRAFT_03g045080) SORBI_03g045090(SORBIDRAFT_03g045090) SORBI_03g045100(SORBIDRAFT_03g045100) SORBI_03g045110(SORBIDRAFT_03g045110) SORBI_09g018440(SORBIDRAFT_09g018440)
ZMA: 
100192086(cl14379_1) 100382663(GRMZM2G461666) 103635265(GRMZM2G047613) 103636584(GRMZM2G440003) 103641536(GRMZM2G086702) 103650797 103651128 103652304
SITA: 
PDA: 
EGU: 
MUS: 
SMO: 
 » show all
Taxonomy
Reference
1
  Authors
Pfitzner, A. and Stockigt, J.
  Title
Characterization of polyneuridine aldehyde esterase, a key enzyme in the biosynthesis of sarpagine ajmaline type alkaloids.
  Journal
Planta Med. 48 (1983) 221-227.
Reference
2
  Authors
Pfitzner, A. and Stockigt, J.
  Title
Polyneuridine aldehyde esterase: an unusual specific enzyme involved in the biosynthesis of sarpagine type alkaloids.
  Journal
J. Chem. Soc. Chem. Commun. (1983) 459-460.
Reference
3  [PMID:10691977]
  Authors
Dogru E, Warzecha H, Seibel F, Haebel S, Lottspeich F, Stockigt J.
  Title
The gene encoding polyneuridine aldehyde esterase of monoterpenoid indole alkaloid biosynthesis in plants is an ortholog of the alpha/betahydrolase super family.
  Journal
Eur. J. Biochem. 267 (2000) 1397-406.
  Sequence
Reference
4  [PMID:12071952]
  Authors
Mattern-Dogru E, Ma X, Hartmann J, Decker H, Stockigt J.
  Title
Potential active-site residues in polyneuridine aldehyde esterase, a central enzyme of indole alkaloid biosynthesis, by modelling and site-directed mutagenesis.
  Journal
Eur. J. Biochem. 269 (2002) 2889-96.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
87041-55-2

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