KEGG   ENZYME: 3.1.1.79Help
Entry
EC 3.1.1.79                 Enzyme                                 
Name
hormone-sensitive lipase;
HSL
Class
Hydrolases;
Acting on ester bonds;
Carboxylic-ester hydrolases
BRITE hierarchy
Sysname
diacylglycerol acylhydrolase
Reaction(IUBMB)
(1) diacylglycerol + H2O = monoacylglycerol + a carboxylate [RN:R05209];
(2) triacylglycerol + H2O = diacylglycerol + a carboxylate [RN:R01369];
(3) monoacylglycerol + H2O = glycerol + a carboxylate [RN:R07293]
Reaction(KEGG)
Substrate
diacylglycerol [CPD:C00165];
H2O [CPD:C00001];
triacylglycerol [CPD:C00422];
monoacylglycerol [CPD:C01885]
Product
monoacylglycerol [CPD:C01885];
carboxylate [CPD:C00060];
diacylglycerol [CPD:C00165];
glycerol [CPD:C00116]
Comment
This enzyme is a serine hydrolase. Compared with other lipases, hormone-sensitive lipase has a uniquely broad substrate specificity. It hydrolyses all acylglycerols (triacylglycerol, diacylglycerol and monoacylglycerol) [2,3,4] as well as cholesteryl esters [2,4], steroid fatty acid esters [5], retinyl esters [6] and p-nitrophenyl esters [4,7]. It exhibits a preference for the 1- or 3-ester bond of its acylglycerol substrate compared with the 2-ester bond [8]. The enzyme shows little preference for the fatty acids in the triacylglycerol, although there is some increase in activity with decreasing chain length. The enzyme activity is increased in response to hormones that elevate intracellular levels of cAMP.
Orthology
K07188  
hormone-sensitive lipase
Genes
HSA: 
3991(LIPE)
PTR: 
456076(LIPE)
PPS: 
100975071(LIPE)
GGO: 
101145804(LIPE)
PON: 
100445800(LIPE)
MMU: 
16890(Lipe)
RNO: 
25330(Lipe)
CFA: 
403607(LIPE)
AML: 
100479605(LIPE)
FCA: 
751818(LIPE)
BTA: 
286879(LIPE)
SSC: 
397583(LIPE)
ECB: 
100069598
SHR: 
100926393(LIPE)
XLA: 
100174796(lipe)
XTR: 
733899(lipe)
DRE: 
557893(lipe)
TRU: 
101070911
OLA: 
101157970
BFO: 
BRAFLDRAFT_89828
CIN: 
100181083
SPU: 
585712
DME: 
Dmel_CG11055
DPO: 
Dpse_GA10731
DAN: 
Dana_GF11751
DER: 
Dere_GG22019
DPE: 
Dper_GL16902
DSE: 
Dsec_GM22000
DSI: 
Dsim_GD11499
DWI: 
Dwil_GK15908
DYA: 
Dyak_GE12096
DGR: 
Dgri_GH23035
DMO: 
Dmoj_GI21105
DVI: 
Dvir_GJ20953
AGA: 
AgaP_AGAP002567
AAG: 
AaeL_AAEL005706
CQU: 
CpipJ_CPIJ015981
AME: 
413702
NVI: 
100120573(NV12945)
TCA: 
664547
API: 
100159927
PHU: 
Phum_PHUM288260
ISC: 
IscW_ISCW018730
CEL: 
CELE_C46C11.1(C46C11.1)
CBR: 
CBG14512
TSP: 
Tsp_02264
SMM: 
Smp_000130.1
NVE: 
NEMVE_v1g20960
HMG: 
100210426
TAD: 
TRIADDRAFT_55258
MBR: 
MONBRDRAFT_25836 MONBRDRAFT_34791
NGR: 
NAEGRDRAFT_57147
TET: 
TTHERM_00185900 TTHERM_00721650 TTHERM_00827140
PIF: 
PITG_10850
 » show all
Taxonomy
Reference
1  [PMID:10940339]
  Authors
Holm C, Osterlund T, Laurell H, Contreras JA.
  Title
Molecular mechanisms regulating hormone-sensitive lipase and lipolysis.
  Journal
Annu. Rev. Nutr. 20 (2000) 365-93.
  Organism
Rattus norvegicus [GN:rno]
Reference
2  [PMID:7240206]
  Authors
Fredrikson G, Stralfors P, Nilsson NO, Belfrage P.
  Title
Hormone-sensitive lipase of rat adipose tissue. Purification and some properties.
  Journal
J. Biol. Chem. 256 (1981) 6311-20.
  Organism
Rattus norvegicus [GN:rno]
Reference
3  [PMID:14169138]
  Authors
VAUGHAN M, BERGER JE, STEINBERG D.
  Title
HORMONE-SENSITIVE LIPASE AND MONOGLYCERIDE LIPASE  ACTIVITIES IN ADIPOSE TISSUE.
  Journal
J. Biol. Chem. 239 (1964) 401-9.
  Organism
Oryctolagus cuniculus
Reference
4  [PMID:8912675]
  Authors
Osterlund T, Danielsson B, Degerman E, Contreras JA, Edgren G, Davis RC, Schotz MC, Holm C.
  Title
Domain-structure analysis of recombinant rat hormone-sensitive lipase.
  Journal
Biochem. J. 319 ( Pt 2) (1996) 411-20.
  Organism
Rattus norvegicus [GN:rno]
Reference
5  [PMID:3196730]
  Authors
Lee FT, Adams JB, Garton AJ, Yeaman SJ.
  Title
Hormone-sensitive lipase is involved in the hydrolysis of lipoidal derivatives of estrogens and other steroid hormones.
  Journal
Biochim. Biophys. Acta. 963 (1988) 258-64.
  Organism
Bos taurus [GN:bta]
Reference
6  [PMID:9162045]
  Authors
Wei S, Lai K, Patel S, Piantedosi R, Shen H, Colantuoni V, Kraemer FB, Blaner WS.
  Title
Retinyl ester hydrolysis and retinol efflux from BFC-1beta adipocytes.
  Journal
J. Biol. Chem. 272 (1997) 14159-65.
  Organism
Bos taurus [GN:bta]
Reference
7  [PMID:2794798]
  Authors
Tsujita T, Ninomiya H, Okuda H.
  Title
p-nitrophenyl butyrate hydrolyzing activity of hormone-sensitive lipase from bovine adipose tissue.
  Journal
J. Lipid. Res. 30 (1989) 997-1004.
  Organism
Bos taurus [GN:bta]
Reference
8  [PMID:14725507]
  Authors
Yeaman SJ.
  Title
Hormone-sensitive lipase--new roles for an old enzyme.
  Journal
Biochem. J. 379 (2004) 11-22.
  Organism
Homo sapiens [GN:hsa], Rattus norvegicus [GN:rno]
Other DBs
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