KEGG   ENZYME: 3.1.1.80Help
Entry
EC 3.1.1.80                 Enzyme                                 

Name
acetylajmaline esterase;
AAE;
2beta(R)-17-O-acetylajmalan:acetylesterase;
acetylajmalan esterase
Class
Hydrolases;
Acting on ester bonds;
Carboxylic-ester hydrolases
BRITE hierarchy
Sysname
17-O-acetylajmaline O-acetylhydrolase
Reaction(IUBMB)
(1) 17-O-acetylajmaline + H2O = ajmaline + acetate [RN:R07513];
(2) 17-O-acetylnorajmaline + H2O = norajmaline + acetate [RN:R05880]
Reaction(KEGG)
Substrate
17-O-acetylajmaline [CPD:C15985];
H2O [CPD:C00001];
17-O-acetylnorajmaline [CPD:C11809]
Product
ajmaline [CPD:C06542];
acetate [CPD:C00033];
norajmaline [CPD:C11810]
Comment
This plant enzyme is responsible for the last stages in the biosynthesis of the indole alkaloid ajmaline. The enzyme is highly specific for the substrates 17-O-acetylajmaline and 17-O-acetylnorajmaline as the structurally related acetylated alkaloids vinorine, vomilenine, 1,2-dihydrovomilenine and 1,2-dihydroraucaffricine cannot act as substrates [2]. This is a novel member of the GDSL family of serine esterases/lipases.
History
EC 3.1.1.80 created 2006
Pathway
Biosynthesis of secondary metabolites
Reference
1  [PMID:2955586]
  Authors
Polz L, Schubel H, Stockigt J.
  Title
Characterization of 2 beta (R)-17-O-acetylajmalan: acetylesterase--a specific enzyme involved in the biosynthesis of the Rauwolfia alkaloid ajmaline.
  Journal
Z. Naturforsch. [C]. 42 (1987) 333-42.
  Organism
Rauwolfia serpentina
Reference
2  [PMID:16133216]
  Authors
Ruppert M, Woll J, Giritch A, Genady E, Ma X, Stockigt J.
  Title
Functional expression of an ajmaline pathway-specific esterase from Rauvolfia in a novel plant-virus expression system.
  Journal
Planta. 222 (2005) 888-98.
  Organism
Rauwolfia serpentina
  Sequence
[up:Q3MKY2]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
110183-46-5

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