KEGG   ENZYME: 3.1.1.80Help
Entry
EC 3.1.1.80                 Enzyme                                 

Name
acetylajmaline esterase;
AAE;
2beta(R)-17-O-acetylajmalan:acetylesterase;
acetylajmalan esterase
Class
Hydrolases;
Acting on ester bonds;
Carboxylic-ester hydrolases
BRITE hierarchy
Sysname
17-O-acetylajmaline O-acetylhydrolase
Reaction(IUBMB)
(1) 17-O-acetylajmaline + H2O = ajmaline + acetate [RN:R07513];
(2) 17-O-acetylnorajmaline + H2O = norajmaline + acetate [RN:R05880]
Reaction(KEGG)
Substrate
17-O-acetylajmaline [CPD:C15985];
H2O [CPD:C00001];
17-O-acetylnorajmaline [CPD:C11809]
Product
ajmaline [CPD:C06542];
acetate [CPD:C00033];
norajmaline [CPD:C11810]
Comment
This plant enzyme is responsible for the last stages in the biosynthesis of the indole alkaloid ajmaline. The enzyme is highly specific for the substrates 17-O-acetylajmaline and 17-O-acetylnorajmaline as the structurally related acetylated alkaloids vinorine, vomilenine, 1,2-dihydrovomilenine and 1,2-dihydroraucaffricine cannot act as substrates [2]. This is a novel member of the GDSL family of serine esterases/lipases.
History
EC 3.1.1.80 created 2006
Pathway
Indole alkaloid biosynthesis
Biosynthesis of secondary metabolites
Orthology
K21026  
acetylajmaline esterase
Genes
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AIP: 
LJA: 
Lj0g3v0257729.1(Lj0g3v0257729.1) Lj6g3v1995900.1(Lj6g3v1995900.1) Lj6g3v1995900.2(Lj6g3v1995900.2) Lj6g3v1995910.1(Lj6g3v1995910.1)
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DOSA: 
Os01t0214600-01(Os01g0214600) Os01t0215000-01(Os01g0215000) Os01t0215700-01(Os01g0215700) Os01t0216400-00(Os01g0216400) Os01t0216500-00(Os01g0216500) Os01t0216900-00(Os01g0216900) Os01t0223000-01(Os01g0223000) Os01t0649200-01(Os01g0649200) Os01t0649400-00(Os01g0649400) Os01t0649900-01(Os01g0649900) Os01t0650200-01(Os01g0650200) Os01t0651000-01(Os01g0651000) Os02t0604000-00(Os02g0604000) Os02t0608801-00(Os02g0608801) Os03t0365000-00(Os03g0365000) Os03t0365800-01(Os03g0365800) Os03t0365900-01(Os03g0365900) Os05t0209600-01(Os05g0209600) Os05t0210100-01(Os05g0210100) Os05t0210400-00(Os05g0210400) Os05t0506500-00(Os05g0506500) Os06t0156600-01(Os06g0156600) Os06t0157000-01(Os06g0157000) Os06t0694200-01(Os06g0694200) Os07t0642200-01(Os07g0642200) Os10t0394000-01(Os10g0394000) Os11t0521000-01(Os11g0521000)
OBR: 
BDI: 
ATS: 
109732539(LOC109732539) 109733106(LOC109733106) 109733108(LOC109733108) 109733109(LOC109733109) 109733111(LOC109733111) 109733876(LOC109733876) 109735703(LOC109735703) 109738813(LOC109738813) 109739194(LOC109739194) 109740480(LOC109740480) 109749898(LOC109749898) 109752266(LOC109752266) 109752451(LOC109752451) 109752461(LOC109752461) 109754960(LOC109754960) 109754967(LOC109754967) 109755025(LOC109755025) 109755030(LOC109755030) 109756443(LOC109756443) 109756639(LOC109756639) 109756981(LOC109756981) 109760236(LOC109760236) 109762707(LOC109762707) 109762719(LOC109762719) 109763796(LOC109763796) 109766488(LOC109766488) 109766493(LOC109766493) 109768677(LOC109768677) 109768679(LOC109768679) 109769318(LOC109769318) 109770611(LOC109770611) 109770612(LOC109770612) 109770797(LOC109770797) 109771041(LOC109771041) 109771283(LOC109771283) 109772848(LOC109772848) 109773388(LOC109773388) 109773650(LOC109773650) 109776349(LOC109776349) 109776350(LOC109776350) 109776351(LOC109776351) 109776352(LOC109776352) 109776738(LOC109776738) 109776739(LOC109776739) 109777174(LOC109777174) 109778302(LOC109778302) 109779234(LOC109779234) 109779544(LOC109779544) 109779548(LOC109779548) 109781428(LOC109781428) 109781860(LOC109781860) 109782174(LOC109782174) 109783948(LOC109783948) 109785760(LOC109785760) 109785762(LOC109785762) 109785923(LOC109785923)
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 » show all
Taxonomy
Reference
1  [PMID:2955586]
  Authors
Polz L, Schubel H, Stockigt J.
  Title
Characterization of 2 beta (R)-17-O-acetylajmalan: acetylesterase--a specific enzyme involved in the biosynthesis of the Rauwolfia alkaloid ajmaline.
  Journal
Z. Naturforsch. [C]. 42 (1987) 333-42.
Reference
2  [PMID:16133216]
  Authors
Ruppert M, Woll J, Giritch A, Genady E, Ma X, Stockigt J.
  Title
Functional expression of an ajmaline pathway-specific esterase from Rauvolfia in a novel plant-virus expression system.
  Journal
Planta. 222 (2005) 888-98.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
110183-46-5

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