KEGG   ENZYME: 3.1.1.88Help
Entry
EC 3.1.1.88                 Enzyme                                 

Name
pyrethroid hydrolase;
pyrethroid-hydrolyzing carboxylesterase;
pyrethroid-hydrolysing esterase;
pyrethroid-hydrolyzing esterase;
pyrethroid-selective esterase;
pyrethroid-cleaving enzyme;
permethrinase;
PytH;
EstP
Class
Hydrolases;
Acting on ester bonds;
Carboxylic-ester hydrolases
BRITE hierarchy
Sysname
pyrethroid-ester hydrolase
Reaction(IUBMB)
trans-permethrin + H2O = (3-phenoxyphenyl)methanol + (1S,3R)-3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropanecarboxylate [RN:R09724]
Reaction(KEGG)
Substrate
trans-permethrin [CPD:C19864];
H2O [CPD:C00001]
Product
(3-phenoxyphenyl)methanol [CPD:C19800];
(1S,3R)-3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropanecarboxylate [CPD:C19844]
Comment
The enzyme is involved in degradation of pyrethroid pesticides. The enzymes from Sphingobium sp., Klebsiella sp. and Aspergillus niger hydrolyse cis-permethrin at approximately equal rate to trans-permethrin [1-3]. The enzyme from mouse hydrolyses trans-permethrin at a rate about 22-fold higher than cis-permethrin [4].
History
EC 3.1.1.88 created 2011
Orthology
K20992  
pyrethroid hydrolase
K20993  
pyrethroid hydrolase
Genes
SEC: 
SEW: 
PMAN: 
SWD: 
EBA: 
SPHB: 
Taxonomy
Reference
1  [PMID:19581484]
  Authors
Wang BZ, Guo P, Hang BJ, Li L, He J, Li SP
  Title
Cloning of a novel pyrethroid-hydrolyzing carboxylesterase gene from Sphingobium  sp. strain JZ-1 and characterization of the gene product.
  Journal
Appl. Environ. Microbiol. 75 (2009) 5496-500.
  Sequence
Reference
2  [PMID:16448191]
  Authors
Wu PC, Liu YH, Wang ZY, Zhang XY, Li H, Liang WQ, Luo N, Hu JM, Lu JQ, Luan TG, Cao LX
  Title
Molecular cloning, purification, and biochemical characterization of a novel pyrethroid-hydrolyzing esterase from Klebsiella sp. strain ZD112.
  Journal
J. Agric. Food. Chem. 54 (2006) 836-42.
  Sequence
Reference
3  [PMID:16159167]
  Authors
Liang WQ, Wang ZY, Li H, Wu PC, Hu JM, Luo N, Cao LX, Liu YH
  Title
Purification and characterization of a novel pyrethroid hydrolase from Aspergillus niger ZD11.
  Journal
J. Agric. Food. Chem. 53 (2005) 7415-20.
Reference
4  [PMID:15123619]
  Authors
Stok JE, Huang H, Jones PD, Wheelock CE, Morisseau C, Hammock BD
  Title
Identification, expression, and purification of a pyrethroid-hydrolyzing carboxylesterase from mouse liver microsomes.
  Journal
J. Biol. Chem. 279 (2004) 29863-9.
  Sequence
Reference
5  [PMID:8357241]
  Authors
Maloney SE, Maule A, Smith AR
  Title
Purification and preliminary characterization of permethrinase from a pyrethroid-transforming strain of Bacillus cereus.
  Journal
Appl. Environ. Microbiol. 59 (1993) 2007-13.
Reference
6
  Authors
Guo, P., Wang, B., Hang, B., Li, L., Ali, W., He, J. and Li, S.
  Title
Pyrethroid-degrading Sphingobium sp. JZ-2 and the purification and characterization of a novel pyrethroid hydrolase.
  Journal
Int. Biodeter. Biodegrad. 63 (2009) 1107-1112.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
UM-BBD (Biocatalysis/Biodegradation Database): 
BRENDA, the Enzyme Database: 

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