KEGG   ENZYME: 3.1.2.29Help
Entry
EC 3.1.2.29                 Enzyme                                 

Name
fluoroacetyl-CoA thioesterase
Class
Hydrolases;
Acting on ester bonds;
Thioester hydrolases
BRITE hierarchy
Sysname
fluoroacetyl-CoA hydrolase
Reaction(IUBMB)
fluoroacetyl-CoA + H2O = fluoroacetate + CoA [RN:R09643]
Reaction(KEGG)
Substrate
fluoroacetyl-CoA [CPD:C19775];
H2O [CPD:C00001]
Product
fluoroacetate [CPD:C06108];
CoA [CPD:C00010]
Comment
Fluoroacetate is extremely toxic. It reacts with CoA to form fluoroacetyl-CoA, which substitutes for acetyl CoA and reacts with EC 2.3.3.1 (citrate synthase) to produce fluorocitrate, a metabolite of which binds very tightly to EC 4.2.1.3 (aconitase) and halts the TCA cycle. This enzyme hydrolyses fluoroacetyl-CoA before it can react with citrate synthase, and thus confers fluoroacetate resistance on the organisms that produce it. It has been described in the poisonous plant Dichapetalum cymosum and the bacterium Streptomyces cattleya, both of which are fluoroacetate producers.
History
EC 3.1.2.29 created 2011
Orthology
K18700  
fluoroacetyl-CoA thioesterase
Genes
DJI: 
PSJ: 
RPJ: 
REU: 
REH: 
H16_A0188(h16_A0188)
CNC: 
CTI: 
CGD: 
BCT: 
PPK: 
PPNO: 
PPNM: 
PRB: 
PPUL: 
PSPU: 
PAPI: 
PTX: 
PNR: 
MASW: 
SLT: 
GCA: 
BPRC: 
GBM: 
GEB: 
AFW: 
DAO: 
DTI: 
RPX: 
OCA: 
OCG: 
OCO: 
HDN: 
HDT: 
HMC: 
HNI: 
FIL: 
FIY: 
RHZ: 
MCG: 
RRU: 
RRF: 
DOR: 
DMI: 
CGL: 
NCgl0442(Cgl0457)
CGB: 
CGU: 
CGT: 
CGS: 
CGG: 
CGM: 
CGJ: 
CGQ: 
CGX: 
CEF: 
CCN: 
CFN: 
CVT: 
CDX: 
SCT: 
SCY: 
SNA: 
MAC: 
MVC: 
MEK: 
 » show all
Taxonomy
Reference
1
  Authors
Meyer, J.J.M., Grobbelaar, N., Vleggaar, R. and Louw, A.I.
  Title
Fluoroacetyl-coenzyme-A hydrolase-like activity in Dichapetalum cymosum.
  Journal
J. Plant Physiol. 139 (1992) 369-372.
Reference
2  [PMID:16720268]
  Authors
Huang F, Haydock SF, Spiteller D, Mironenko T, Li TL, O'Hagan D, Leadlay PF, Spencer JB
  Title
The gene cluster for fluorometabolite biosynthesis in Streptomyces cattleya: a thioesterase confers resistance to fluoroacetyl-coenzyme A.
  Journal
Chem. Biol. 13 (2006) 475-84.
  Sequence
[sct:SCAT_4159]
Reference
3  [PMID:20430898]
  Authors
Dias MV, Huang F, Chirgadze DY, Tosin M, Spiteller D, Dry EF, Leadlay PF, Spencer JB, Blundell TL
  Title
Structural basis for the activity and substrate specificity of fluoroacetyl-CoA thioesterase FlK.
  Journal
J. Biol. Chem. 285 (2010) 22495-504.
  Sequence
[sct:SCAT_4159]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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