EC 18.104.22.168 Enzyme
Streptomyces glaucescens exocytoplasmic dodeoxyribonuclease
Acting on ester bonds;
Endodeoxyribonucleases producing 5'-phosphomonoesters
endonucleolytic cleavage to give 5'-phosphooligonucleotide end-products, with a preference for cleavage within the sequence CC
Prefers CC sites in double-stranded circular and linear DNA. Greater affinity for double-stranded than single-stranded DNA. Produces nicks, generating double-stranded fragments with 5'- and/or 3'-protruding single-stranded tails. Requires magnesium ions for activity. The endonuclease from Chlorella-like green algae infected with NYs-1 virus 4 may be the same enzyme.
EC 22.214.171.124 created 1999
Xia YN, Morgan R, Schildkraut I, Van Etten JL.
A site-specific single strand endonuclease activity induced by NYs-1 virus infection of a Chlorella-like green alga.
Nucleic. Acids. Res. 16 (1988) 9477-87.
Aparicio JF, Freije JM, Lopez-Otin C, Cal S, Sanchez J.
A Streptomyces glaucescens endodeoxyribonuclease which shows a strong preference for CC dinucleotide.
Eur. J. Biochem. 205 (1992) 695-9.
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