KEGG   ENZYME: 3.1.21.8Help
Entry
EC 3.1.21.8                 Enzyme                                 

Name
T4 deoxyribonuclease II;
T4 endonuclease II;
EndoII (ambiguous);
denA (gene name)
Class
Hydrolases;
Acting on ester bonds;
Endodeoxyribonucleases producing 5'-phosphomonoesters
BRITE hierarchy
Reaction(IUBMB)
Endonucleolytic nicking and cleavage of cytosine-containing double-stranded DNA.
Comment
Requires Mg2+. This phage T4 enzyme is involved in degradation of host DNA. The enzyme primarily catalyses nicking of the bottom strand of double stranded DNA between the first and second base pair to the right of a top-strand CCGC motif. Double-stranded breaks are produced 5- to 10-fold less frequently [3]. It does not cleave the T4 native DNA, which contains 5-hydroxymethylcytosine instead of cytosine.
History
EC 3.1.21.8 created 2014
Reference
1  [PMID:8386173]
  Authors
Carlson K, Krabbe M, Nystrom AC, Kosturko LD
  Title
DNA determinants of restriction. Bacteriophage T4 endonuclease II-dependent cleavage of plasmid DNA in vivo.
  Journal
J. Biol. Chem. 268 (1993) 8908-18.
  Sequence
[vg:1258710]
Reference
2  [PMID:9515694]
  Authors
Carlson K, Kosturko LD
  Title
Endonuclease II of coliphage T4: a recombinase disguised as a restriction endonuclease?
  Journal
Mol. Microbiol. 27 (1998) 671-6.
Reference
3  [PMID:10200960]
  Authors
Carlson K, Kosturko LD, Nystrom AC
  Title
Sequence-specific cleavage by bacteriophage T4 endonuclease II in vitro.
  Journal
Mol. Microbiol. 31 (1999) 1395-405.
Reference
4  [PMID:20156453]
  Authors
Andersson CE, Lagerback P, Carlson K
  Title
Structure of bacteriophage T4 endonuclease II mutant E118A, a tetrameric GIY-YIG  enzyme.
  Journal
J. Mol. Biol. 397 (2010) 1003-16.
  Sequence
[vg:1258710]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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