KEGG   ENZYME: 3.1.21.9Help
Entry
EC 3.1.21.9                 Enzyme                                 

Name
T4 deoxyribonuclease IV;
T4 endonuclease IV;
EndoIV (ambiguous);
denB (gene name)
Class
Hydrolases;
Acting on ester bonds;
Endodeoxyribonucleases producing 5'-phosphomonoesters
BRITE hierarchy
Reaction(IUBMB)
Endonucleolytic cleavage of the 5' phosphodiester bond of deoxycytidine in single-stranded DNA.
Comment
This phage T4 enzyme is involved in degradation of host DNA. The enzyme does not cleave double-stranded DNA or native T4 DNA, which contains 5-hydroxymethylcytosine instead of cytosine.
History
EC 3.1.21.9 created 2014
Orthology
K20842  
T4 deoxyribonuclease IV
Reference
1  [PMID:4900512]
  Authors
Sadowski PD, Hurwitz J
  Title
Enzymatic breakage of deoxyribonucleic acid. II. Purification and properties of endonuclease IV from T4 phage-infected Escherichia coli.
  Journal
J. Biol. Chem. 244 (1969) 6192-8.
Reference
2  [PMID:11946172]
  Authors
Ling V
  Title
Partial digestion of 32P-fd DNA with T(4) endonuclease IV.
  Journal
FEBS. Lett. 19 (1971) 50-54.
Reference
3  [PMID:4550601]
  Authors
Sadowski PD, Bakyta I
  Title
T4 endonuclease IV. Improved purification procedure and resolution from T4 endonuclease 3.
  Journal
J. Biol. Chem. 247 (1972) 405-12.
Reference
4  [PMID:782881]
  Authors
Bernardi A, Maat J, de Waard A, Bernardi G
  Title
Preparation and specificity of endonuclease IV induced by bacteriophage T4.
  Journal
Eur. J. Biochem. 66 (1976) 175-9.
Reference
5  [PMID:16971463]
  Authors
Hirano N, Ohshima H, Takahashi H
  Title
Biochemical analysis of the substrate specificity and sequence preference of endonuclease IV from bacteriophage T4, a dC-specific endonuclease implicated in restriction of dC-substituted T4 DNA synthesis.
  Journal
Nucleic. Acids. Res. 34 (2006) 4743-51.
  Sequence
[vg:1258726]
Reference
6  [PMID:17940096]
  Authors
Ohshima H, Hirano N, Takahashi H
  Title
A hexanucleotide sequence (dC1-dC6 tract) restricts the dC-specific cleavage of single-stranded DNA by endonuclease IV of bacteriophage T4.
  Journal
Nucleic. Acids. Res. 35 (2007) 6681-9.
  Sequence
[vg:1258726]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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