KEGG   ENZYME: 3.1.4.50Help
Entry
EC 3.1.4.50                 Enzyme                                 
Name
glycosylphosphatidylinositol phospholipase D;
GPI-PLD;
glycoprotein phospholipase D;
phosphatidylinositol phospholipase D;
phosphatidylinositol-specific phospholipase D
Class
Hydrolases;
Acting on ester bonds;
Phosphoric-diester hydrolases
BRITE hierarchy
Sysname
glycoprotein-phosphatidylinositol phosphatidohydrolase
Reaction(IUBMB)
6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O = 6-(alpha-D-glucosaminyl)-1D-myo-inositol + 3-sn-phosphatidate [RN:R06623]
Reaction(KEGG)
R06623(G);
(other) R04149 R07398
Show
Substrate
6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol [CPD:C04248];
H2O [CPD:C00001]
Product
6-(alpha-D-glucosaminyl)-1D-myo-inositol [CPD:C15658];
3-sn-phosphatidate [CPD:C00416]
Comment
This enzyme is also active when O-4 of the glucosamine is substituted by carrying the oligosaccharide that can link a protein to the structure. It therefore cleaves proteins from the lipid part of the glycosylphosphatidylinositol (GPI) anchors, but does so by hydrolysis, whereas glycosylphosphatidylinositol diacylglycerol-lyase (EC 4.6.1.14) does so by elimination. It acts on plasma membranes only after solubilization of the substrate with detergents or solvents, but it may act on intracellular membranes.
Pathway
Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
Orthology
K01127  
glycosylphosphatidylinositol phospholipase D
Genes
HSA: 
2822(GPLD1)
PTR: 
462473(GPLD1)
PPS: 
100973223(GPLD1)
GGO: 
101146393(GPLD1)
PON: 
100455517(GPLD1)
MCC: 
707782(GPLD1)
MMU: 
14756(Gpld1)
RNO: 
291132(Gpld1)
CFA: 
478735(GPLD1)
AML: 
100484842(GPLD1)
FCA: 
101088486(GPLD1)
BTA: 
287025(GPLD1)
SSC: 
100157497
ECB: 
100067517(GPLD1)
MDO: 
100024296
SHR: 
100931811(GPLD1)
OAA: 
100092985(GPLD1)
GGA: 
420819(GPLD1)
MGP: 
100546397(GPLD1)
TGU: 
100230775(GPLD1)
ACS: 
100567850(gpld1)
XTR: 
100493665
BFO: 
BRAFLDRAFT_241888
SPU: 
590298
NVE: 
NEMVE_v1g115107
SCE: 
YJL132W
LTH: 
KLTH0F05830g
PPA: 
PAS_chr4_0595
ZRO: 
ZYRO0D03256g
CGR: 
CAGL0L05720g
YLI: 
YALI0B15972g
TML: 
GSTUM_00002772001
DDI: 
DDB_G0276919(pldG)
NOC: 
Noc_2092 Noc_2093
 » show all
Taxonomy
Reference
1  [PMID:3422494]
  Authors
Low MG, Prasad AR.
  Title
A phospholipase D specific for the phosphatidylinositol anchor of cell-surface proteins is abundant in plasma.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 85 (1988) 980-4.
  Organism
Homo sapiens [GN:hsa]
Reference
2  [PMID:3028377]
  Authors
Malik AS, Low MG.
  Title
Conversion of human placental alkaline phosphatase from a high Mr form to a low Mr form during butanol extraction. An investigation of the role of endogenous phosphoinositide-specific phospholipases.
  Journal
Biochem. J. 240 (1986) 519-27.
  Organism
Homo sapiens [GN:hsa]
Reference
3  [PMID:7961859]
  Authors
Li JY, Hollfelder K, Huang KS, Low MG.
  Title
Structural features of GPI-specific phospholipase D revealed by proteolytic fragmentation and Ca2+ binding studies.
  Journal
J. Biol. Chem. 269 (1994) 28963-71.
  Organism
Bos taurus [GN:bta]
Reference
4  [PMID:11254757]
  Authors
Deeg MA, Bierman EL, Cheung MC.
  Title
GPI-specific phospholipase D associates with an apoA-I- and apoA-IV-containing complex.
  Journal
J. Lipid. Res. 42 (2001) 442-51.
  Organism
Homo sapiens [GN:hsa]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
113756-14-2
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