KEGG   ENZYME: 3.1.4.54Help
Entry
EC 3.1.4.54                 Enzyme                                 

Name
N-acetylphosphatidylethanolamine-hydrolysing phospholipase D;
NAPE-PLD;
anandamide-generating phospholipase D;
N-acyl phosphatidylethanolamine phospholipase D;
NAPE-hydrolyzing phospholipase D
Class
Hydrolases;
Acting on ester bonds;
Phosphoric-diester hydrolases
BRITE hierarchy
Sysname
N-acetylphosphatidylethanolamine phosphatidohydrolase
Reaction(IUBMB)
N-acylphosphatidylethanolamine + H2O = N-acylethanolamine + a 1,2-diacylglycerol 3-phosphate
Substrate
N-acylphosphatidylethanolamine;
H2O [CPD:C00001]
Product
N-acylethanolamine;
1,2-diacylglycerol 3-phosphate
Comment
This enzyme is involved in the biosynthesis of anandamide. It does not hydrolyse phosphatidylcholine and phosphatidylethanolamine [1]. No transphosphatidation [1]. The enzyme contains Zn2+ and is activated by Mg2+ or Ca2+ [2].
History
EC 3.1.4.54 created 2011
Orthology
K13985  
N-acyl-phosphatidylethanolamine-hydrolysing phospholipase D
Genes
HSA: 
222236(NAPEPLD)
PTR: 
472476(NAPEPLD)
PPS: 
100985918(NAPEPLD)
GGO: 
101137253(NAPEPLD)
PON: 
100172099(NAPEPLD)
NLE: 
100597657(NAPEPLD)
MCC: 
695318(NAPEPLD)
MCF: 
101866055(NAPEPLD)
RRO: 
104673548(NAPEPLD)
CJC: 
100398969(NAPEPLD)
MMU: 
242864(Napepld)
RNO: 
296757(Napepld)
CGE: 
100759349(Napepld)
NGI: 
103732204(Napepld)
HGL: 
OCU: 
100352578(NAPEPLD)
TUP: 
102497728(NAPEPLD)
CFA: 
100685330(NAPEPLD)
AML: 
100480963(NAPEPLD)
UMR: 
103658645(NAPEPLD)
FCA: 
101090597(NAPEPLD)
PTG: 
102971600(NAPEPLD)
BTA: 
541291(NAPEPLD)
BOM: 
102267222(NAPEPLD)
PHD: 
102320957(NAPEPLD)
CHX: 
102189544(NAPEPLD)
OAS: 
101119039(NAPEPLD)
SSC: 
100514175(NAPEPLD)
CFR: 
102512680(NAPEPLD)
BACU: 
103008372(NAPEPLD)
LVE: 
103084546(NAPEPLD)
ECB: 
100050247(NAPEPLD)
MYB: 
102251197(NAPEPLD)
MYD: 
102751565(NAPEPLD)
PALE: 
102890709(NAPEPLD)
MDO: 
100017822(NAPEPLD)
SHR: 
100922754(NAPEPLD)
OAA: 
100076416(NAPEPLD)
GGA: 
417719(NAPEPLD)
MGP: 
100544476(NAPEPLD)
CJO: 
107323995(NAPEPLD)
APLA: 
101804191(NAPEPLD)
TGU: 
100232210(NAPEPLD)
GFR: 
102032062(NAPEPLD)
FAB: 
101814848(NAPEPLD)
PHI: 
102109609(NAPEPLD)
CCW: 
104686879(NAPEPLD)
FPG: 
101919393(NAPEPLD)
FCH: 
102047018(NAPEPLD)
CLV: 
102087617(NAPEPLD)
AAM: 
106485772(NAPEPLD)
ASN: 
102388069(NAPEPLD)
AMJ: 
102564505(NAPEPLD)
PSS: 
102451163(NAPEPLD)
CMY: 
102934785(NAPEPLD)
ACS: 
100559466(napepld)
PBI: 
103056311(NAPEPLD)
GJA: 
107113713(NAPEPLD)
XLA: 
100137693(napepld)
XTR: 
100494898(napepld)
DRE: 
568061(napepld)
TRU: 
101065463(napepld)
MZE: 
101469541(napepld)
OLA: 
101162968(napepld)
XMA: 
102223768(napepld)
LCM: 
102353963(NAPEPLD)
CMK: 
103176983(napepld)
SPU: 
SKO: 
ISC: 
CEL: 
CBR: 
TSP: 
CRG: 
OBI: 
NVE: 
HMG: 
TAD: 
SMO: 
CRE: 
CSL: 
CVR: 
APRO: 
CME: 
GSL: 
CCP: 
CNB: 
CGI: 
TMS: 
CCI: 
MLR: 
SRE: 
DDI: 
DPP: 
DFA: 
ACAN: 
PTI: 
SPAR: 
GTT: 
NGR: 
ADI: 
OAI: 
 » show all
Taxonomy
Reference
1  [PMID:14634025]
  Authors
Okamoto Y, Morishita J, Tsuboi K, Tonai T, Ueda N
  Title
Molecular characterization of a phospholipase D generating anandamide and its congeners.
  Journal
J. Biol. Chem. 279 (2004) 5298-305.
  Sequence
[hsa:222236] [mmu:242864] [rno:296757]
Reference
2  [PMID:16527816]
  Authors
Wang J, Okamoto Y, Morishita J, Tsuboi K, Miyatake A, Ueda N
  Title
Functional analysis of the purified anandamide-generating phospholipase D as a member of the metallo-beta-lactamase family.
  Journal
J. Biol. Chem. 281 (2006) 12325-35.
  Sequence
[rno:296757] [mmu:242864] [hsa:222236]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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