KEGG   ENZYME: 3.2.1.107Help
Entry
EC 3.2.1.107                Enzyme                                 

Name
protein-glucosylgalactosylhydroxylysine glucosidase;
PGGHG (gene name);
2-O-alpha-D-glucopyranosyl-5-O-alpha-D-galactopyranosylhydroxy-L-lysine glucohydrolase;
protein-alpha-D-glucosyl-1,2-beta-D-galactosyl-L-hydroxylysine glucohydrolase;
protein-alpha-D-glucosyl-(1->2)-beta-D-galactosyl-L-hydroxylysine glucohydrolase
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
BRITE hierarchy
Sysname
[collagen]-(5R)-5-O-[alpha-D-glucosyl-(1->2)-beta-D-galactosyl]-5-hydroxy-L-lysine glucohydrolase
Reaction(IUBMB)
[collagen]-(5R)-5-O-[alpha-D-glucosyl-(1->2)-beta-D-galactosyl]-5-hydroxy-L-lysine + H2O = D-glucose + [collagen]-(5R)-5-O-(beta-D-galactosyl)-5-hydroxy-L-lysine [RN:R04503]
Reaction(KEGG)
Substrate
[collagen]-(5R)-5-O-[alpha-D-glucosyl-(1->2)-beta-D-galactosyl]-5-hydroxy-L-lysine;
H2O [CPD:C00001]
Product
D-glucose [CPD:C00031];
[collagen]-(5R)-5-O-(beta-D-galactosyl)-5-hydroxy-L-lysine
Comment
The enzyme specifically hydrolyses glucose from alpha-D-glucosyl-(1->2)-beta-D-galactosyl disaccharide units that are linked to hydroxylysine residues of collagen and collagen-like proteins. Acetylation of the epsilon-amino group of the glycosylated hydroxylysine abolishes activity.
History
EC 3.2.1.107 created 1984
Orthology
K22078  
protein-glucosylgalactosylhydroxylysine glucosidase
Genes
HSA: 
80162(PGGHG)
PTR: 
743704(PGGHG)
PPS: 
100971508(PGGHG)
GGO: 
109025438(PGGHG)
PON: 
NLE: 
100601940(PGGHG)
MCC: 
697199(PGGHG)
MCF: 
101926478(PGGHG)
CSAB: 
103236799(PGGHG)
RRO: 
104655560(PGGHG)
RBB: 
108537993(PGGHG)
CJC: 
100385744(PGGHG)
SBQ: 
101049147(ATHL1)
MMU: 
212974(Pgghg)
RNO: 
309103(Pgghg)
CGE: 
100757546(Athl1)
NGI: 
103751062(Pgghg)
HGL: 
101723837(Pgghg)
CCAN: 
109698993(Pgghg)
OCU: 
103347496(PGGHG)
TUP: 
102501509(PGGHG)
CFA: 
483395(PGGHG)
AML: 
100469466(PGGHG)
UMR: 
103671029(PGGHG)
ORO: 
101386980(ATHL1)
FCA: 
101087100(PGGHG)
PTG: 
102949755(PGGHG)
AJU: 
106987954(PGGHG)
BTA: 
511919(ATHL1)
BOM: 
102276155(PGGHG)
PHD: 
102324274(PGGHG)
CHX: 
102188292(PGGHG)
OAS: 
101106382(ATHL1)
SSC: 
100518005(PGGHG)
CFR: 
102511585(PGGHG)
CDK: 
105105739(PGGHG)
BACU: 
103010340(PGGHG)
LVE: 
103071239(PGGHG)
ECB: 
100146757(PGGHG)
EPZ: 
103568056(PGGHG)
EAI: 
106843884(PGGHG)
MYB: 
102252209(PGGHG)
MYD: 
102760826(PGGHG)
HAI: 
109378078(PGGHG)
RSS: 
109439220(PGGHG)
PALE: 
102887768(PGGHG)
LAV: 
100655235(PGGHG)
TMU: 
MDO: 
103092300(PGGHG)
SHR: 
100923289(PGGHG)
OAA: 
100091579(PGGHG)
GGA: 
422991(PGGHG)
MGP: 
100538987(PGGHG)
CJO: 
107314276(PGGHG)
APLA: 
101791880(PGGHG)
ACYG: 
106047048(ATHL1)
TGU: 
100219083(ATHL1) 100221918(ATHL1)
GFR: 
102035352(PGGHG)
FAB: 
101806950(PGGHG)
PHI: 
102112573(PGGHG)
PMAJ: 
107205256(PGGHG)
CCW: 
104684542(PGGHG)
FPG: 
101919210(PGGHG)
FCH: 
102051782(PGGHG)
CLV: 
102089207(PGGHG)
EGZ: 
104127885(ATHL1)
AAM: 
106489147(PGGHG)
ASN: 
102382785(PGGHG)
AMJ: 
102566701(PGGHG)
PSS: 
102452333(PGGHG)
CMY: 
102947357(PGGHG)
CPIC: 
101949278(PGGHG)
ACS: 
100552045(pgghg)
PVT: 
110086184(PGGHG)
PBI: 
103064802(PGGHG)
GJA: 
107112736(PGGHG)
XLA: 
XTR: 
100496278(pgghg)
NPR: 
108784117(PGGHG)
DRE: 
777617(pgghg)
SRX: 
SANH: 
SGH: 
CCAR: 
109071510(pgghg)
IPU: 
108274703(pgghg)
AMEX: 
103031131(pgghg)
TRU: 
101062094(pgghg)
TNG: 
LCO: 
104928845(pgghg)
NCC: 
104956195(athl1)
MZE: 
101468880(pgghg)
OLA: 
101155845(pgghg)
XMA: 
102229355(pgghg)
PRET: 
103466501(pgghg)
NFU: 
107388675(athl1)
CSEM: 
103380281(athl1)
LCF: 
HCQ: 
109507601(pgghg)
BPEC: 
110169653(pgghg)
SASA: 
106561823(pgghg)
ELS: 
105029689(pgghg)
SFM: 
108940848(pgghg)
LCM: 
102363080(PGGHG)
CMK: 
103174850(pgghg)
BFO: 
CIN: 
SPU: 
APLC: 
SKO: 
DME: 
Dmel_CG16965(CG16965)
DPO: 
DAN: 
DER: 
DPE: 
DSE: 
DSI: 
Dsimw501_GD23801(Dsim_GD23801)
DWI: 
DYA: 
DGR: 
DMO: 
DVI: 
MDE: 
AGA: 
AAG: 
CQU: 
BMOR: 
DPL: 
PMAC: 
PRAP: 
PXY: 
FCD: 
DPX: 
HRO: 
LGI: 
CRG: 
MYI: 
OBI: 
LAK: 
OVI: 
NVE: 
EPA: 
ADF: 
HMG: 
MBR: 
SRE: 
DDI: 
DPP: 
DFA: 
DFA_10677(athl1)
ACAN: 
NGR: 
MUC: 
MGOT: 
MgSA37_02000(kojP_1)
 » show all
Taxonomy
Reference
1  [PMID:385589]
  Authors
Hamazaki H, Hotta K.
  Title
Purification and characterization of an alpha-glucosidase specific for hydroxylysine-linked disaccharide of collagen.
  Journal
J. Biol. Chem. 254 (1979) 9682-7.
Reference
2  [PMID:7460918]
  Authors
Hamazaki H, Hotta K.
  Title
Enzymatic hydrolysis of disaccharide unit of collagen. Isolation of 2-O-alpha-D-glucopyranosyl-O-beta-D-galactopyranosyl-hydroxylysine glucohydrolase from rat spleens.
  Journal
Eur. J. Biochem. 111 (1980) 587-91.
Reference
3  [PMID:385599]
  Authors
Sternberg M, Spiro RG.
  Title
Studies on the catabolism of the hydroxylysine-linked disaccharide units of basement membranes and collagens. Isolation and characterization of a rat kidney alpha-glucosidase of high specificity.
  Journal
J. Biol. Chem. 254 (1979) 10329-36.
Reference
4  [PMID:26682924]
  Authors
Hamazaki H, Hamazaki MH
  Title
Catalytic site of human protein-glucosylgalactosylhydroxylysine glucosidase: Three crucial carboxyl residues were determined by cloning and site-directed mutagenesis.
  Journal
Biochem. Biophys. Res. Commun. 469 (2016) 357-62.
  Sequence
[gga:422991]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
72829-45-9

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