KEGG   ENZYME: 3.2.1.149Help
Entry
EC 3.2.1.149                Enzyme                                 

Name
beta-primeverosidase
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
BRITE hierarchy
Sysname
6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside 6-O-(beta-D-xylosyl)-beta-D-glucohydrolase
Reaction(IUBMB)
a 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O = 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose + an alcohol [RN:R05788]
Reaction(KEGG)
Substrate
6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside [CPD:C11531];
H2O [CPD:C00001]
Product
6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose [CPD:C11535];
alcohol [CPD:C00069]
Comment
The enzyme is responsible for the formation of the alcoholic aroma in oolong and black tea. In addition to beta-primeverosides [i.e. 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranosides], it also hydrolyses 6-O-(beta-D-apiofuranosyl)-beta-D-glucopyranosides and, less rapidly, beta-vicianosides and 6-O-(alpha-L-arabinofuranosyl)-beta-D-glucopyranosides, but not beta-glucosides. Geranyl-, linaloyl-, benzyl- and p-nitrophenol glycosides are all hydrolysed.
History
EC 3.2.1.149 created 2001
Reference
1
  Authors
Ijima, Y., Ogawa, K., Watanabe, N., Usui, T., Ohnishi-Kameyama, M., Nagata, T. and Sakata, K.
  Title
Characterization of beta-primeverosidase, being concerned with alcoholic aroma formation in tea leaves to be processed into black tea, and preliminary observations on its substrate specificity.
  Journal
J. Agric. Food Chem. 46 (1998) 1712-1718.
Reference
2
  Authors
Ogawa, K., Ijima, Y., Guo, W., Watanabe, N., Usui, T., Dong, S., Tong, Q. and Sakata, K.
  Title
Purification of a beta-primeverosidase concerned with alcoholic aroma formation in tea leaves (cv. Shuxian) to be processed to oolong tea.
  Journal
J. Agric. Food Chem. 45 (1997) 877-882.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
884593-92-4

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