KEGG   ENZYME: 3.2.1.154Help
Entry
EC 3.2.1.154                Enzyme                                 

Name
fructan beta-(2,6)-fructosidase;
beta-(2-6)-fructan exohydrolase;
levanase;
6-FEH;
beta-(2,6)-D-fructan fructohydrolase
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
BRITE hierarchy
Sysname
(2->6)-beta-D-fructan fructohydrolase
Reaction(IUBMB)
Hydrolysis of terminal, non-reducing (2->6)-linked beta-D-fructofuranose residues in fructans
Comment
Possesses one of the activities of EC 3.2.1.80, fructan beta-fructosidase. While the best substrates are the levan-type fructans such as 6-kestotriose [beta-D-fructofuranosyl-(2->6)-beta-D-fructofuranosyl alpha-D-glucopyranoside] and 6,6-kestotetraose [beta-D-fructofuranosyl-(2->6)-beta-D-fructofuranosyl-(2->6)-beta-D-fructofuranosyl alpha-D-glucopyranoside], some (but not all) inulin-type fructans can also be hydrolysed, but more slowly [cf. EC 3.2.1.153, fructan beta-(2,1)-fructosidase]. Sucrose, while being a very poor substrate, can substantially inhibit enzyme activity in some cases.
History
EC 3.2.1.154 created 2005
Reference
1
  Authors
Marx, S.P., Nosberger, J. and Frehner, M.
  Title
Hydrolysis of fructan in grasses: A beta-(2-6)-linkage specific fructan-beta-fructosidase from stubble of Lolium perenne.
  Journal
New Phytol. 135 (1997) 279-290.
Reference
2  [PMID:14617070]
  Authors
Van den Ende W, De Coninck B, Clerens S, Vergauwen R, Van Laere A.
  Title
Unexpected presence of fructan 6-exohydrolases (6-FEHs) in non-fructan plants: characterization, cloning, mass mapping and functional analysis of a novel "cell-wall invertase-like" specific 6-FEH from sugar beet (Beta vulgaris L.).
  Journal
Plant. J. 36 (2003) 697-710.
  Sequence
Reference
3  [PMID:8742337]
  Authors
Henson CA, Livingston DP 3rd.
  Title
Purification and characterization of an oat fructan exohydrolase that preferentially hydrolyzes beta-2,6-fructans.
  Journal
Plant. Physiol. 110 (1996) 639-44.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
1000597-62-5

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