KEGG   ENZYME: 3.2.1.158Help
Entry
EC 3.2.1.158                Enzyme                                 

Name
alpha-agarase;
agarase (ambiguous);
agaraseA33
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
BRITE hierarchy
Sysname
agarose 3-glycanohydrolase
Reaction(IUBMB)
Endohydrolysis of (1->3)-alpha-L-galactosidic linkages in agarose, yielding agarotetraose as the major product
Comment
Requires Ca2+. The enzyme from Thalassomonas sp. can use agarose, agarohexaose and neoagarohexaose as substrate. The products of agarohexaose hydrolysis are dimers and tetramers, with agarotetraose being the predominant product, whereas hydrolysis of neoagarohexaose gives rise to two types of trimer. While the enzyme can also hydrolyse the highly sulfated agarose porphyran very efficiently, it cannot hydrolyse the related compounds kappa-carrageenan (see EC 3.2.1.83) and iota-carrageenan (see EC 3.2.1.157) [2]. See also EC 3.2.1.81, beta-agarase.
History
EC 3.2.1.158 created 2006
Reference
1  [PMID:8513809]
  Authors
Potin P, Richard C, Rochas C, Kloareg B.
  Title
Purification and characterization of the alpha-agarase from Alteromonas agarlyticus (Cataldi) comb. nov., strain GJ1B.
  Journal
Eur. J. Biochem. 214 (1993) 599-607.
Reference
2  [PMID:15902469]
  Authors
Ohta Y, Hatada Y, Miyazaki M, Nogi Y, Ito S, Horikoshi K.
  Title
Purification and characterization of a novel alpha-agarase from a Thalassomonas sp.
  Journal
Curr. Microbiol. 50 (2005) 212-6.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
63952-00-1

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