KEGG   ENZYME: 3.2.1.165Help
Entry
EC 3.2.1.165                Enzyme                                 

Name
exo-1,4-beta-D-glucosaminidase;
CsxA;
GlcNase;
exochitosanase;
GlmA;
exo-beta-D-glucosaminidase;
chitosan exo-1,4-beta-D-glucosaminidase
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
BRITE hierarchy
Sysname
chitosan exo-(1->4)-beta-D-glucosaminidase
Reaction(IUBMB)
Hydrolysis of chitosan or chitosan oligosaccharides to remove successive D-glucosamine residues from the non-reducing termini [RN:R06225 R08715]
Reaction(KEGG)
Comment
Chitosan is a partially or totally N-deacetylated chitin derivative that is found in the cell walls of some phytopathogenic fungi and comprises D-glucosamine residues with a variable content of GlcNAc residues [4]. Acts specifically on chitooligosaccharides and chitosan, having maximal activity on chitotetraose, chitopentaose and their corresponding alcohols [1]. The enzyme can degrade GlcN-GlcNAc but not GlcNAc-GlcNAc [3]. A member of the glycoside hydrolase family 2 (GH-2) [4].
History
EC 3.2.1.165 created 2008
Pathway
Amino sugar and nucleotide sugar metabolism
Metabolic pathways
Orthology
K15855  
exo-1,4-beta-D-glucosaminidase
Genes
NCR: 
NTE: 
NEUTE1DRAFT130945(NEUTE1DRAFT_130945)
SMP: 
PAN: 
TTT: 
MTM: 
CTHR: 
MGR: 
FGR: 
FPU: 
TRE: 
MAW: 
MAJ: 
CMT: 
ELA: 
ANI: 
AFM: 
AOR: 
AOR_1_2480154(AO090003001410)
ANG: 
ANI_1_2638014(An01g06630)
AFV: 
ACT: 
NFI: 
PCS: 
URE: 
ABE: 
TVE: 
PNO: 
PTE: 
BZE: 
BSC: 
BOR: 
PFJ: 
SRE: 
DJI: 
AGE: 
HPK: 
MSA: 
MCB: 
MYV: 
MYE: 
MGO: 
MFT: 
SRT: 
SMA: 
SAV_1223(csxA)
SVL: 
SBH: 
SBI_00935(bga4)
SHY: 
SHO: 
SCI: 
SRC: 
SCX: 
SRW: 
TUE45_00966(csxA_1)
AMD: 
AMN: 
AMM: 
AMZ: 
AOI: 
AJA: 
ALU: 
SESP: 
BN6_82410(csxA)
KAL: 
KPHY: 
CAI: 
ABA: 
ACA: 
TRS: 
SUS: 
GBA: 
BCEL: 
PRU: 
SHG: 
RSI: 
ZPR: 
 » show all
Taxonomy
Reference
1  [PMID:2351651]
  Authors
Nanjo F, Katsumi R, Sakai K.
  Title
Purification and characterization of an exo-beta-D-glucosaminidase, a novel type of enzyme, from Nocardia orientalis.
  Journal
J. Biol. Chem. 265 (1990) 10088-94.
  Sequence
[aoi:AORI_5124]
Reference
2  [PMID:16349528]
  Authors
Nogawa M, Takahashi H, Kashiwagi A, Ohshima K, Okada H, Morikawa Y.
  Title
Purification and Characterization of Exo-beta-d-Glucosaminidase from a Cellulolytic Fungus, Trichoderma reesei PC-3-7.
  Journal
Appl. Environ. Microbiol. 64 (1998) 890-895.
  Sequence
Reference
3  [PMID:16877749]
  Authors
Fukamizo T, Fleury A, Cote N, Mitsutomi M, Brzezinski R.
  Title
Exo-beta-D-glucosaminidase from Amycolatopsis orientalis: catalytic residues, sugar recognition specificity, kinetics, and synergism.
  Journal
Glycobiology. 16 (2006) 1064-72.
Reference
4  [PMID:16316314]
  Authors
Cote N, Fleury A, Dumont-Blanchette E, Fukamizo T, Mitsutomi M, Brzezinski R.
  Title
Two exo-beta-D-glucosaminidases/exochitosanases from actinomycetes define a new subfamily within family 2 of glycoside hydrolases.
  Journal
Biochem. J. 394 (2006) 675-86.
  Sequence
Reference
5  [PMID:16636831]
  Authors
Ike M, Isami K, Tanabe Y, Nogawa M, Ogasawara W, Okada H, Morikawa Y.
  Title
Cloning and heterologous expression of the exo-beta-D-glucosaminidase-encoding gene (gls93) from a filamentous fungus, Trichoderma reesei PC-3-7.
  Journal
Appl. Microbiol. Biotechnol. 72 (2006) 687-95.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

DBGET integrated database retrieval system