KEGG   ENZYME: 3.2.1.171Help
Entry
EC 3.2.1.171                Enzyme                                 

Name
rhamnogalacturonan hydrolase;
rhamnogalacturonase A;
RGase A;
RG-hydrolase
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
BRITE hierarchy
Sysname
rhamnogalacturonan alpha-D-GalA-(1->2)-alpha-L-Rha hydrolase
Reaction(IUBMB)
Endohydrolysis of alpha-D-GalA-(1->2)-alpha-L-Rha glycosidic bond in the rhamnogalacturonan I backbone with initial inversion of anomeric configuration releasing oligosaccharides with beta-D-GalA at the reducing end.
Comment
The enzyme is part of the degradation system for rhamnogalacturonan I in Aspergillus aculeatus.
History
EC 3.2.1.171 created 2011
Orthology
K18580  
rhamnogalacturonan hydrolase
Genes
TTT: 
TMN: 
FGR: 
NHE: 
SSL: 
BFU: 
ANI: 
AFM: 
AOR: 
AOR_1_130174(AO090005000067) AOR_1_14104(AO090124000009) AOR_1_470014(AO090026000252) AOR_1_798024(AO090010000484) AOR_1_936074(AO090038000552)
ANG: 
ANI_1_1364104(An12g00950) ANI_1_604124(An14g04200)
AFV: 
NFI: 
PTE: 
ZTR: 
BCOM: 
NPA: 
TML: 
PPL: 
DSQ: 
PCO: 
SHS: 
HIR: 
PSQ: 
ADL: 
FME: 
GTR: 
CPUT: 
SLA: 
PFP: 
PGR: 
MLR: 
 » show all
Taxonomy
Reference
1  [PMID:9115442]
  Authors
Petersen TN, Kauppinen S, Larsen S
  Title
The crystal structure of rhamnogalacturonase A from Aspergillus aculeatus: a right-handed parallel beta helix.
  Journal
Structure. 5 (1997) 533-44.
  Sequence
Reference
2  [PMID:7961884]
  Authors
Kofod LV, Kauppinen S, Christgau S, Andersen LN, Heldt-Hansen HP, Dorreich K, Dalboge H
  Title
Cloning and characterization of two structurally and functionally divergent rhamnogalacturonases from Aspergillus aculeatus.
  Journal
J. Biol. Chem. 269 (1994) 29182-9.
  Sequence
Reference
3  [PMID:8720076]
  Authors
Azadi P, O'Neill MA, Bergmann C, Darvill AG, Albersheim P
  Title
The backbone of the pectic polysaccharide rhamnogalacturonan I is cleaved by an endohydrolase and an endolyase.
  Journal
Glycobiology. 5 (1995) 783-9.
  Sequence
Reference
4  [PMID:15299976]
  Authors
Petersen TN, Christgau S, Kofod LV, Kauppinen S, Johnson AH, Larsen S
  Title
Crystallization and preliminary X-ray studies of rhamnogalacturonase A from Aspergillus aculeatus.
  Journal
Acta. Crystallogr. D. Biol. Crystallogr. 53 (1997) 105-7.
Reference
5  [PMID:9464254]
  Authors
Pitson SM, Mutter M, van den Broek LA, Voragen AG, Beldman G
  Title
Stereochemical course of hydrolysis catalysed by alpha-L-rhamnosyl and alpha-D-galacturonosyl hydrolases from Aspergillus aculeatus.
  Journal
Biochem. Biophys. Res. Commun. 242 (1998) 552-9.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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