KEGG   ENZYME: 3.2.1.187Help
Entry
EC 3.2.1.187                Enzyme                                 

Name
(Ara-f)3-Hyp beta-L-arabinobiosidase;
hypBA2 (gene name);
beta-L-arabinobiosidase
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
BRITE hierarchy
Sysname
4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose hydrolase
Reaction(IUBMB)
4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + H2O = 4-O-(beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose [RN:R10579]
Reaction(KEGG)
Substrate
4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline [CPD:C20705];
H2O [CPD:C00001]
Product
4-O-(beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline [CPD:C20706];
beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose [CPD:C20568]
Comment
The enzyme, which was identified in the bacterium Bifidobacterium longum JCM1217, is specific for (Ara-f)3-Hyp, a sugar chain found in hydroxyproline-rich glyoproteins such as extensin and lectin. The enzyme was not able to accept (Ara-f)2-Hyp or (Ara-f)4-Hyp as substrates. In the presence of 1-alkanols, the enzyme demonstrates transglycosylation activity, retaining the anomeric configuration of the arabinofuranose residue.
History
EC 3.2.1.187 created 2013
Orthology
K18206  
beta-L-arabinobiosidase
Genes
BLO: 
BLF: 
BLB: 
BLM: 
BLK: 
BLG: 
BDE: 
BDP_1701(bbgIII)
BDN: 
Taxonomy
Reference
1  [PMID:21149454]
  Authors
Fujita K, Sakamoto S, Ono Y, Wakao M, Suda Y, Kitahara K, Suganuma T
  Title
Molecular cloning and characterization of a beta-L-Arabinobiosidase in Bifidobacterium longum that belongs to a novel glycoside hydrolase family.
  Journal
J. Biol. Chem. 286 (2011) 5143-50.
  Sequence
[blm:BLLJ_0212]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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