KEGG   ENZYME: 3.2.1.191Help
Entry
EC 3.2.1.191                Enzyme                                 

Name
ginsenosidase type III
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
BRITE hierarchy
Sysname
protopanaxadiol-type ginsenoside 3-beta-D-hydrolase
Reaction(IUBMB)
a protopanaxadiol-type ginsenoside with two glucosyl residues at position 3 + 2 H2O = a protopanaxadiol-type ginsenoside with no glycosidic modification at position 3 + 2 D-glucopyranose (overall reaction);
(1a) a protopanaxadiol-type ginsenoside with two glucosyl residues at position 3 + H2O a protopanaxadiol-type ginsenoside with one glucosyl residue at position 3 + D-glucopyranose;
(1b) a protopanaxadiol-type ginsenoside with one glucosyl residue at position 3 + H2O = a protopanaxadiol-type ginsenoside with no glycosidic modification at position 3 + D-glucopyranose
Substrate
protopanaxadiol-type ginsenoside with two glucosyl residues at position 3;
H2O [CPD:C00001];
protopanaxadiol-type ginsenoside with one glucosyl residue at position 3
Product
protopanaxadiol-type ginsenoside with no glycosidic modification at position 3;
D-glucopyranose [CPD:C00031]
Comment
Ginsenosidase type III catalyses the sequential hydrolysis of the 3-O-beta-D-(1->2)-glucopyranosyl bond followed by hydrolysis of the 3-O-beta-D-glucopyranosyl bond of protopanaxadiol ginsenosides. When acting for example on ginsenoside Rb1 the enzyme first generates ginsenoside XVII, and subsequently ginsenoside LXXV.
History
EC 3.2.1.191 created 2014
Reference
1  [PMID:22450790]
  Authors
Jin XF, Yu HS, Wang DM, Liu TQ, Liu CY, An DS, Im WT, Kim SG, Jin FX
  Title
Kinetics of a cloned special ginsenosidase hydrolyzing 3-O-glucoside of multi-protopanaxadiol-type ginsenosides, named ginsenosidase type III.
  Journal
J. Microbiol. Biotechnol. 22 (2012) 343-51.
  Sequence
Reference
2  [PMID:20622122]
  Authors
An DS, Cui CH, Lee HG, Wang L, Kim SC, Lee ST, Jin F, Yu H, Chin YW, Lee HK, Im WT, Kim SG
  Title
Identification and characterization of a novel Terrabacter ginsenosidimutans sp.  nov. beta-glucosidase that transforms ginsenoside Rb1 into the rare gypenosides XVII and LXXV.
  Journal
Appl. Environ. Microbiol. 76 (2010) 5827-36.
  Sequence
Reference
3  [PMID:23717145]
  Authors
Hong H, Cui CH, Kim JK, Jin FX, Kim SC, Im WT
  Title
Enzymatic Biotransformation of Ginsenoside Rb1 and Gypenoside XVII into Ginsenosides Rd and F2 by Recombinant beta-glucosidase from Flavobacterium johnsoniae.
  Journal
J. Ginseng. Res. 36 (2012) 418-24.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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