KEGG   ENZYME: 3.2.1.191Help
Entry
EC 3.2.1.191                Enzyme                                 

Name
ginsenosidase type III
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
BRITE hierarchy
Sysname
protopanaxadiol-type ginsenoside 3-beta-D-hydrolase
Reaction(IUBMB)
a protopanaxadiol-type ginsenoside with two glucosyl residues at position 3 + 2 H2O = a protopanaxadiol-type ginsenoside with no glycosidic modification at position 3 + 2 D-glucopyranose (overall reaction);
(1a) a protopanaxadiol-type ginsenoside with two glucosyl residues at position 3 + H2O a protopanaxadiol-type ginsenoside with one glucosyl residue at position 3 + D-glucopyranose;
(1b) a protopanaxadiol-type ginsenoside with one glucosyl residue at position 3 + H2O = a protopanaxadiol-type ginsenoside with no glycosidic modification at position 3 + D-glucopyranose
Substrate
protopanaxadiol-type ginsenoside with two glucosyl residues at position 3;
H2O [CPD:C00001];
protopanaxadiol-type ginsenoside with one glucosyl residue at position 3
Product
protopanaxadiol-type ginsenoside with no glycosidic modification at position 3;
D-glucopyranose [CPD:C00031]
Comment
Ginsenosidase type III catalyses the sequential hydrolysis of the 3-O-beta-D-(1->2)-glucopyranosyl bond followed by hydrolysis of the 3-O-beta-D-glucopyranosyl bond of protopanaxadiol ginsenosides. When acting for example on ginsenoside Rb1 the enzyme first generates ginsenoside XVII, and subsequently ginsenoside LXXV.
History
EC 3.2.1.191 created 2014
Orthology
K20836  
ginsenosidase type III
Reference
1  [PMID:22450790]
  Authors
Jin XF, Yu HS, Wang DM, Liu TQ, Liu CY, An DS, Im WT, Kim SG, Jin FX
  Title
Kinetics of a cloned special ginsenosidase hydrolyzing 3-O-glucoside of multi-protopanaxadiol-type ginsenosides, named ginsenosidase type III.
  Journal
J. Microbiol. Biotechnol. 22 (2012) 343-51.
  Sequence
Reference
2  [PMID:20622122]
  Authors
An DS, Cui CH, Lee HG, Wang L, Kim SC, Lee ST, Jin F, Yu H, Chin YW, Lee HK, Im WT, Kim SG
  Title
Identification and characterization of a novel Terrabacter ginsenosidimutans sp.  nov. beta-glucosidase that transforms ginsenoside Rb1 into the rare gypenosides XVII and LXXV.
  Journal
Appl. Environ. Microbiol. 76 (2010) 5827-36.
  Sequence
Reference
3  [PMID:23717145]
  Authors
Hong H, Cui CH, Kim JK, Jin FX, Kim SC, Im WT
  Title
Enzymatic Biotransformation of Ginsenoside Rb1 and Gypenoside XVII into Ginsenosides Rd and F2 by Recombinant beta-glucosidase from Flavobacterium johnsoniae.
  Journal
J. Ginseng. Res. 36 (2012) 418-24.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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