KEGG   ENZYME: 3.2.1.194Help
Entry
EC 3.2.1.194                Enzyme                                 

Name
ginsenosidase type IV
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
BRITE hierarchy
Sysname
protopanaxatriol-type ginsenoside 6-beta-D-glucohydrolase
Reaction(IUBMB)
a protopanaxatriol-type ginsenoside with two glycosyl residues at position 6 + 2 H2O = a protopanaxatriol-type ginsenoside with no glycosidic modification at position 6 + D-glucopyranose + a monosaccharide (overall reaction);
(1a) a protopanaxatriol-type ginsenoside with two glycosyl residues at position 6 + H2O = a protopanaxatriol-type ginsenoside with a single glucosyl at position 6 + a monosaccharide;
(1b) a protopanaxatriol-type ginsenoside with a single glucosyl at position 6 + H2O = a protopanaxatriol-type ginsenoside with no glycosidic modification at position 6 + D-glucopyranose
Substrate
protopanaxatriol-type ginsenoside with two glycosyl residues at position 6;
H2O [CPD:C00001];
protopanaxatriol-type ginsenoside with a single glucosyl at position 6
Product
protopanaxatriol-type ginsenoside with no glycosidic modification at position 6;
D-glucopyranose [CPD:C00031];
monosaccharide;
protopanaxatriol-type ginsenoside with a single glucosyl at position 6
Comment
Ginsenosidase type IV catalyses the sequential hydrolysis of the 6-O-beta-D-(1->2)-glycosidic bond or the 6-O-alpha-D-(1->2)-glycosidic bond in protopanaxatriol-type ginsenosides with a disacchride attached to the C6 position, followed by the hydrolysis of the remaining 6-O-beta-D-glycosidic bond (e.g. ginsenoside Re -> ginsenoside Rg1 -> ginsenoside F1).
History
EC 3.2.1.194 created 2014
Reference
1  [PMID:22031031]
  Authors
Wang DM, Yu HS, Song JG, Xu YF, Liu CY, Jin FX
  Title
A novel ginsenosidase from an Aspergillus strain hydrolyzing 6-O-multi-glycosides of protopanaxatriol-type ginsenosides, named ginsenosidase type IV.
  Journal
J. Microbiol. Biotechnol. 21 (2011) 1057-63.
Reference
2
  Authors
Wang, D, Yu, H., Song, J., Xu, Y., Jin, F.
  Title
Enzyme kinetics of ginsenosidase type IV hydrolyzing 6-O-multi-glycosides of protopanaxatriol type ginsenosides.
  Journal
Process Biochem. 47 (2012) 133-138.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

DBGET integrated database retrieval system