KEGG   ENZYME: 3.2.1.83Help
Entry
EC 3.2.1.83                 Enzyme                                 

Name
kappa-carrageenase;
kappa-carrageenan 4-beta-D-glycanohydrolase
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
BRITE hierarchy
Sysname
kappa-carrageenan 4-beta-D-glycanohydrolase (configuration-retaining)
Reaction(IUBMB)
Endohydrolysis of (1->4)-beta-D-linkages between D-galactose 4-sulfate and 3,6-anhydro-D-galactose in kappa-carrageenans
Comment
The main products of hydrolysis are neocarrabiose-sulfate and neocarratetraose-sulfate [5]. Unlike EC 3.2.1.157 (iota-carrageenase), but similar to EC 3.2.1.81 (beta-agarase), this enzyme proceeds with retention of the anomeric configuration.
History
EC 3.2.1.83 created 1972, modified 2006
Orthology
K05990  
kappa-carrageenase
Genes
RBA: 
Taxonomy
Reference
1  [PMID:5972647]
  Authors
Weigl J, Yaphe W.
  Title
The enzymic hydrolysis of carrageenan by Pseudomonas carrageenovora: purification of a kappa-carrageenase.
  Journal
Can. J. Microbiol. 12 (1966) 939-47.
  Organism
Pseudomonas carrageenovora
Reference
2  [PMID:1915370]
  Authors
Potin P, Sanseau A, Le Gall Y, Rochas C, Kloareg B.
  Title
Purification and characterization of a new kappa-carrageenase from a marine Cytophaga-like bacterium.
  Journal
Eur. J. Biochem. 201 (1991) 241-7.
  Organism
Delesseria sanguinea
Reference
3  [PMID:7737202]
  Authors
Potin P, Richard C, Barbeyron T, Henrissat B, Gey C, Petillot Y, Forest E, Dideberg O, Rochas C, Kloareg B.
  Title
Processing and hydrolytic mechanism of the cgkA-encoded kappa-carrageenase of Alteromonas carrageenovora.
  Journal
Eur. J. Biochem. 228 (1995) 971-5.
  Organism
Alteromonas carrageenovora
Reference
4  [PMID:10089334]
  Authors
Michel G, Barbeyron T, Flament D, Vernet T, Kloareg B, Dideberg O.
  Title
Expression, purification, crystallization and preliminary x-ray analysis of the kappa-carrageenase from Pseudoalteromonas carrageenovora.
  Journal
Acta. Crystallogr. D. Biol. Crystallogr. 55 (1999) 918-20.
  Organism
Pseudomonas carrageenovora
Reference
5  [PMID:11435116]
  Authors
Michel G, Chantalat L, Duee E, Barbeyron T, Henrissat B, Kloareg B, Dideberg O.
  Title
The kappa-carrageenase of P. carrageenovora features a tunnel-shaped active site: a novel insight in the evolution of Clan-B glycoside hydrolases.
  Journal
Structure. 9 (2001) 513-25.
  Organism
Pseudomonas carrageenovora
  Sequence
[up:P43478]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
37288-59-8

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