| Entry |
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| Name |
kappa-carrageenase;
kappa-carrageenan 4-beta-D-glycanohydrolase
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| Class |
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
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| Sysname |
kappa-carrageenan 4-beta-D-glycanohydrolase (configuration-retaining)
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| Reaction(IUBMB) |
Endohydrolysis of (1->4)-beta-D-linkages between D-galactose 4-sulfate and 3,6-anhydro-D-galactose in kappa-carrageenans
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| Comment |
The main products of hydrolysis are neocarrabiose-sulfate and neocarratetraose-sulfate [5]. Unlike EC 3.2.1.157 (iota-carrageenase), but similar to EC 3.2.1.81 (beta-agarase), this enzyme proceeds with retention of the anomeric configuration.
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| Orthology |
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| Genes |
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| Reference |
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| Authors |
Weigl J, Yaphe W. |
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| Title |
The enzymic hydrolysis of carrageenan by Pseudomonas carrageenovora: purification of a kappa-carrageenase. |
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| Journal |
Can. J. Microbiol. 12 (1966) 939-47. |
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| Organism |
Pseudomonas carrageenovora |
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| Reference |
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| Authors |
Potin P, Sanseau A, Le Gall Y, Rochas C, Kloareg B. |
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| Title |
Purification and characterization of a new kappa-carrageenase from a marine Cytophaga-like bacterium. |
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| Journal |
Eur. J. Biochem. 201 (1991) 241-7. |
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| Organism |
Delesseria sanguinea |
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| Reference |
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| Authors |
Potin P, Richard C, Barbeyron T, Henrissat B, Gey C, Petillot Y, Forest E, Dideberg O, Rochas C, Kloareg B. |
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| Title |
Processing and hydrolytic mechanism of the cgkA-encoded kappa-carrageenase of Alteromonas carrageenovora. |
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| Journal |
Eur. J. Biochem. 228 (1995) 971-5. |
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| Organism |
Alteromonas carrageenovora |
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| Reference |
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| Authors |
Michel G, Barbeyron T, Flament D, Vernet T, Kloareg B, Dideberg O. |
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| Title |
Expression, purification, crystallization and preliminary x-ray analysis of the kappa-carrageenase from Pseudoalteromonas carrageenovora. |
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| Journal |
Acta. Crystallogr. D. Biol. Crystallogr. 55 (1999) 918-20. |
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| Organism |
Pseudomonas carrageenovora |
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| Reference |
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| Authors |
Michel G, Chantalat L, Duee E, Barbeyron T, Henrissat B, Kloareg B, Dideberg O. |
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| Title |
The kappa-carrageenase of P. carrageenovora features a tunnel-shaped active site: a novel insight in the evolution of Clan-B glycoside hydrolases. |
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| Journal |
Structure. 9 (2001) 513-25. |
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| Organism |
Pseudomonas carrageenovora |
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| Other DBs |
ExplorEnz - The Enzyme Database: IUBMB Enzyme Nomenclature: ExPASy - ENZYME nomenclature database: BRENDA, the Enzyme Database: CAS: 37288-59-8 |
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