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Entry
EC 3.2.2.28                 Enzyme                                 

Name
double-stranded uracil-DNA glycosylase;
Mug;
double-strand uracil-DNA glycosylase;
Dug;
dsUDG;
double-stranded DNA specific UDG;
dsDNA specific UDG;
UdgB (ambiguous);
G:T/U mismatch-specific DNA glycosylase;
UDG (ambiguous)
Class
Hydrolases;
Glycosylases;
Hydrolysing N-glycosyl compounds
BRITE hierarchy
Sysname
uracil-double-stranded DNA deoxyribohydrolase (uracil-releasing)
Reaction(IUBMB)
Specifically hydrolyses mismatched double-stranded DNA and polynucleotides, releasing free uracil
Comment
No activity on DNA containing a T/G mispair or single-stranded DNA containing either a site-specific uracil or 3,N4-ethenocytosine residue [2], significant role for double-stranded uracil-DNA glycosylase in mutation avoidance in non-dividing E. coli [3]. Uracil-DNA glycosylases are widespread enzymes that are found in all living organisms. Uracil-DNA glycosylase (EC 3.2.2.27) and EC 3.2.2.28 form a central part of the DNA-repair machinery since they initiate the DNA base-excision repair pathway by hydrolysing the N-glycosidic bond between uracil and the deoxyribose sugar thereby catalysing the removal of mis-incorporated uracil from DNA.
History
EC 3.2.2.28 created 2009
Reference
1  [PMID:10581234]
  Authors
Barrett TE, Scharer OD, Savva R, Brown T, Jiricny J, Verdine GL, Pearl LH
  Title
Crystal structure of a thwarted mismatch glycosylase DNA repair complex.
  Journal
EMBO. J. 18 (1999) 6599-609.
  Sequence
[eco:b3068]
Reference
2  [PMID:10956012]
  Authors
Sung JS, Mosbaugh DW
  Title
Escherichia coli double-strand uracil-DNA glycosylase: involvement in uracil-mediated DNA base excision repair and stimulation of activity by endonuclease IV.
  Journal
Biochemistry. 39 (2000) 10224-35.
Reference
3  [PMID:11555290]
  Authors
Mokkapati SK, Fernandez de Henestrosa AR, Bhagwat AS
  Title
Escherichia coli DNA glycosylase Mug: a growth-regulated enzyme required for mutation avoidance in stationary-phase cells.
  Journal
Mol. Microbiol. 41 (2001) 1101-11.
  Sequence
[eco:b3068]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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