KEGG   ENZYME: 3.2.2.8Help
Entry
EC 3.2.2.8                  Enzyme                                 

Name
ribosylpyrimidine nucleosidase;
N-ribosylpyrimidine nucleosidase;
pyrimidine nucleosidase;
N-ribosylpyrimidine ribohydrolase;
pyrimidine nucleoside hydrolase;
RihB;
YeiK;
nucleoside ribohydrolase
Class
Hydrolases;
Glycosylases;
Hydrolysing N-glycosyl compounds
BRITE hierarchy
Sysname
pyrimidine-nucleoside ribohydrolase
Reaction(IUBMB)
a pyrimidine nucleoside + H2O = D-ribose + a pyrimidine base [RN:R02172]
Reaction(KEGG)
Substrate
pyrimidine nucleoside [CPD:C03169];
H2O [CPD:C00001]
Product
D-ribose [CPD:C00121];
pyrimidine base [CPD:C00396]
Comment
Also hydrolyses purine D-ribonucleosides, but much more slowly. 2'-, 3'- and 5'-deoxynucleosides are not substrates [3].
History
EC 3.2.2.8 created 1972
Pathway
Purine metabolism
Pyrimidine metabolism
Metabolic pathways
Orthology
K10213  
ribosylpyrimidine nucleosidase
Genes
ECO: 
b2162(rihB)
ECJ: 
JW2149(rihB)
ECD: 
EBW: 
BWG_1944(rihB)
ECOK: 
ECE: 
Z3419(yeiK)
ECS: 
ECs3054(rihB)
ECF: 
ETW: 
ECSP_3040(rihB)
ELX: 
EOJ: 
EOI: 
EOH: 
ECG: 
EOK: 
ELR: 
ECC: 
c2696(yeiK)
ECP: 
ECI: 
ECV: 
ECX: 
ECW: 
ECM: 
ECY: 
ECR: 
ECQ: 
ECK: 
ECT: 
EOC: 
CE10_2534(rihB)
EUM: 
ECZ: 
ELO: 
ELN: 
ELH: 
ESE: 
ESO: 
O3O_17000(rihB)
ESM: 
O3M_08585(rihB)
ESL: 
O3K_08635(rihB)
ECL: 
EBR: 
ECB_02091(rihB)
EBD: 
EKO: 
EKF: 
EAB: 
EDH: 
EDJ: 
EIH: 
ENA: 
ELU: 
EUN: 
ELW: 
ECW_m2363(rihB)
ELL: 
WFL_11560(rihB)
ELC: 
i14_2497(rihB)
ELD: 
i02_2497(rihB)
ELP: 
EBL: 
ECD_02091(rihB)
EBE: 
B21_02050(rihB)
ELF: 
LF82_1884(rihB)
ECOA: 
ECOL: 
ECOI: 
ECOJ: 
ECOO: 
ECOH: 
ECOS: 
EFE: 
EFER_2248(rihB)
SES: 
SFL: 
SF2247(rihB)
SFX: 
S2376(yeiK)
SFV: 
SFV_2237(yeiK)
SFE: 
SFxv_2480(rihB)
SFN: 
SFS: 
SFT: 
SSN: 
SSON_2218(yeiK)
SSJ: 
SBO: 
SBO_2165(yeiK)
SBC: 
SDY: 
SDY_2104(yeiK)
SDZ: 
SHQ: 
HAV: 
OPO: 
LBR: 
LBK: 
RUM: 
CSH: 
OVA: 
OBV_11660(rihB)
MBJ: 
KQ51_00964(rihB_1)
AMQ: 
BDE: 
BDP_1920(iunH)
BDN: 
SBU: 
SCC: 
SGP: 
BRM: 
BPJ: 
BPW: 
LBA: 
LEO: 
STR: 
TAI: 
 » show all
Taxonomy
Reference
1  [PMID:12325375]
  Authors
Terada M, Tatibana M, Hayaishi O.
  Title
Purification and properties of nucleoside hydrolase from Pseudomonas fluorescens.
  Journal
J. Biol. Chem. 242 (1967) 5578-85.
Reference
2  [PMID:11027694]
  Authors
Petersen C, Moller LB.
  Title
The RihA, RihB, and RihC ribonucleoside hydrolases of Escherichia coli. Substrate specificity, gene expression, and regulation.
  Journal
J. Biol. Chem. 276 (2001) 884-94.
  Sequence
[eco:b2162]
Reference
3  [PMID:14993681]
  Authors
Giabbai B, Degano M.
  Title
Cloning, purification, crystallization and X-ray analysis of the Escherichia coli pyrimidine nucleoside hydrolase YeiK.
  Journal
Acta. Crystallogr. D. Biol. Crystallogr. 60 (2004) 524-7.
Reference
4  [PMID:15130467]
  Authors
Giabbai B, Degano M.
  Title
Crystal structure to 1.7 a of the Escherichia coli pyrimidine nucleoside hydrolase YeiK, a novel candidate for cancer gene therapy.
  Journal
Structure. 12 (2004) 739-49.
  Sequence
[eco:b2162]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
37288-60-1

DBGET integrated database retrieval system