KEGG   ENZYME: 3.3.2.8Help
Entry
EC 3.3.2.8                  Enzyme                                 

Name
limonene-1,2-epoxide hydrolase;
limonene oxide hydrolase
Class
Hydrolases;
Acting on ether bonds;
Ether hydrolases
BRITE hierarchy
Sysname
1,2-epoxymenth-8-ene hydrolase
Reaction(IUBMB)
1,2-epoxymenth-8-ene + H2O = menth-8-ene-1,2-diol [RN:R05784 R09387]
Reaction(KEGG)
Substrate
1,2-epoxymenth-8-ene;
H2O [CPD:C00001]
Product
menth-8-ene-1,2-diol [CPD:C18020]
Comment
Involved in the monoterpene degradation pathway of the actinomycete Rhodococcus erythropolis. The enzyme hydrolyses several alicyclic and 1-methyl-substituted epoxides, such as 1-methylcyclohexene oxide, indene oxide and cyclohexene oxide. It differs from the previously described epoxide hydrolases [EC 3.3.2.4 (trans-epoxysuccinate hydrolase), EC 3.3.2.6 (leukotriene-A4 hydrolase), EC 3.3.2.7 (hepoxilin-epoxide hydrolase), EC 3.3.2.9 (microsomal epoxide hydrolase) and EC 3.3.2.10 (soluble epoxide hydrolase)] as it is not inhibited by 2-bromo-4'-nitroacetophenone, diethyl dicarbonate, 4-fluorochalcone oxide or 1,10-phenanthroline. Both enantiomers of menth-8-ene-1,2-diol [i.e. (1R,2R,4S)-menth-8-ene-1,2-diol and (1S,2S,4R)-menth-8-ene-1,2-diol] are metabolized.
History
EC 3.3.2.8 created 2001
Pathway
Limonene and pinene degradation
Orthology
K10533  
limonene-1,2-epoxide hydrolase
Genes
ECA: 
PATR: 
PATO: 
DSO: 
PFE: 
OSG: 
CYQ: 
CZA: 
OME: 
PSPI: 
RSO: 
RSc2589(limA) RSc2590(limA2)
RPJ: 
RIN: 
CNC: 
BVI: 
BVE: 
BCN: 
BCH: 
BCM: 
BCEN: 
BAC: 
BGE: 
BUK: 
RBU: 
SAMY: 
LLU: 
MRM: 
PLA: 
ATF: 
AGR: 
REL: 
REI: 
RLG: 
RPHA: 
CCR: 
CCS: 
CAK: 
CHQ: 
PZU: 
CBOT: 
HNE: 
HBC: 
NPN: 
NRE: 
SMAG: 
SMAZ: 
SWI: 
SJP: 
SCH: 
SYB: 
SBD: 
SPMI: 
SPHT: 
SPHG: 
SFLA: 
BLAS: 
ELQ: 
AMX: 
ANH: 
CMAN: 
PNS: 
PORL: 
ASZ: 
MTU: 
Rv2740(ephG)
MTV: 
MRA: 
MTF: 
MTB: 
MTK: 
MTZ: 
MTG: 
MTI: 
MTE: 
MTUR: 
MTL: 
MTO: 
MTD: 
MTN: 
MTJ: 
MTUB: 
MTUC: 
MTUE: 
MTX: 
MTUH: 
MTUL: 
MTUT: 
MTUU: 
MTQ: 
MBO: 
MBB: 
BCG_2754(ephG)
MBT: 
JTY_2748(ephG)
MBM: 
MBK: 
MBX: 
MBZ: 
MAF: 
MCE: 
MCQ: 
MCV: 
MCX: 
MCZ: 
MMIC: 
MLE: 
MLB: 
MPA: 
MAO: 
MAVI: 
MAVU: 
MAV: 
MAVD: 
MAVR: 
MAVA: 
MIT: 
MIA: 
MIE: 
MIR: 
MID: 
MYO: 
MCHI: 
MSM: 
MSG: 
MSB: 
MSN: 
MSH: 
MSA: 
MUL: 
MVA: 
MGI: 
MSP: 
MAB: 
MABB: 
MMV: 
MAY: 
MABO: 
MABL: 
MAZ: 
MAK: 
MYS: 
MYC: 
MCHE: 
MIZ: 
MMC: 
MKM: 
MJL: 
MJD: 
MMI: 
MMAE: 
MRH: 
MMM: 
MCB: 
MLI: 
MKN: 
MKS: 
MKI: 
MNE: 
MYV: 
MYE: 
MGO: 
MFT: 
MHAD: 
MPHL: 
MVQ: 
ASD: 
NFA: 
NFR: 
NCY: 
NBR: 
NNO: 
NSL: 
NSR: 
RHA: 
RER: 
REY: 
REB: 
ROP: 
ROA: 
REQ: 
RPY: 
RHB: 
RAV: 
RFA: 
RHW: 
GBR: 
GPO: 
GOR: 
GOQ: 
GTA: 
TPR: 
SBH: 
SXI: 
STRC: 
SPAV: 
NCA: 
NDK: 
I601_1587(ephG)
FRI: 
FAL: 
FSY: 
AMD: 
AMN: 
AMM: 
AMZ: 
AOI: 
AJA: 
AMQ: 
ALU: 
PDX: 
KAL: 
LED: 
AHM: 
AYM: 
TPX: 
 » show all
Taxonomy
Reference
1  [PMID:9748436]
  Authors
van der Werf MJ, Overkamp KM, de Bont JA.
  Title
Limonene-1,2-epoxide hydrolase from Rhodococcus erythropolis DCL14 belongs to a novel class of epoxide hydrolases.
  Journal
J. Bacteriol. 180 (1998) 5052-7.
Reference
2  [PMID:9827564]
  Authors
Barbirato F, Verdoes JC, de Bont JA, van der Werf MJ.
  Title
The Rhodococcus erythropolis DCL14 limonene-1,2-epoxide hydrolase gene encodes an enzyme belonging to a novel class of epoxide hydrolases.
  Journal
FEBS. Lett. 438 (1998) 293-6.
  Sequence
Reference
3  [PMID:10224006]
  Authors
van der Werf MJ, Swarts HJ, de Bont JA.
  Title
Rhodococcus erythropolis DCL14 contains a novel degradation pathway for limonene.
  Journal
Appl. Environ. Microbiol. 65 (1999) 2092-102.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
UM-BBD (Biocatalysis/Biodegradation Database): 
BRENDA, the Enzyme Database: 
CAS: 
216503-88-7

DBGET integrated database retrieval system