KEGG   ENZYME: 3.4.17.21Help
Entry
EC 3.4.17.21                Enzyme                                 
Name
glutamate carboxypeptidase II;
N-acetylated-gamma-linked-acidic dipeptidase (NAALADase);
folate hydrolase;
prostate-specific membrane antigen;
pteroylpoly-gamma-glutamate carboxypeptidase;
microsomal gamma-glutamyl carboxypeptidase;
pteroylpolyglutamate hydrolase;
folylpolyglutamate hydrolase;
pteroylpoly-gamma-glutamate hydrolase;
pteroylpolygammaglutamyl hydrolase;
pteroylpolyglutamate hydrolase;
pteroylpolyglutamic acid hydrolase;
PSM antigen;
acetylaspartylglutamate dipeptidase;
NAALA dipeptidase;
rat NAAG peptidase;
mGCP;
membrane glutamate carboxypeptidase;
N-acetylated-alpha-linked-amino dipeptidase;
prostrate-specific membrane antigen;
N-Acetylated alpha-linked acidic dipeptidase;
PSMA
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Metallocarboxypeptidases
BRITE hierarchy
Reaction(IUBMB)
Release of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly-gamma-glutamates
Comment
A metallo-carboxypeptidase that is predominantly expressed as a membrane-bound enzyme of 94-100 kDa , but also exists in a soluble form. Hydrolyses alpha-peptide bonds in Ac-Asp-Glu, Asp-Glu, and Glu-Glu, but also gamma-glutamyl bonds in gamma-Glu-Glu, and folylpoly-gamma-glutamates. With folylpoly-gamma-glutamates, shows processive carboxypeptidase activity to produce pteroylmonoglutamate [4]. Does not hydrolyse Ac-beta-Asp-Glu. Known inhibitors: quisqualic acid, Ac-beta-Asp-Glu, and 2-phosphonomethyl-pentanedioate. In peptidase family M28 of Vibrio leucyl aminopeptidase. The release of C-terminal glutamate from folylpoly-gamma-glutamates is also catalysed by EC 3.4.17.11 (glutamate carboxypeptidase) and EC 3.4.19.9 (gamma-Glu-X carboxypeptidase).
Orthology
K01301  
glutamate carboxypeptidase II
K14592  
folate hydrolase 1
Genes
HSA: 
10003(NAALAD2) 10004(NAALADL1) 2346(FOLH1)
PTR: 
451185(FOLH1) 451309(NAALADL1) 451475 451478(NAALAD2)
PPS: 
100970655(NAALAD2) 100970744(NAALADL1)
GGO: 
101133710(NAALADL1) 101147888(FOLH1)
PON: 
100445995(NAALADL1) 100453848(FOLH1) 100460916(NAALAD2)
MCC: 
694180(NAALAD2) 707714(FOLH1) 717018(NAALADL1)
MMU: 
381204(Naaladl1) 53320(Folh1) 72560(Naalad2)
RNO: 
300384(Naalad2) 83568(Naaladl1) 85309(Folh1)
CFA: 
476775(FOLH1) 483751(NAALADL1) 485139(NAALAD2)
AML: 
100469236(NAALAD2) 100470423(NAALADL1) 100472805
FCA: 
101082777(NAALADL1) 101089389(NAALAD2) 101089641
BTA: 
505865(FOLH1) 506692(NAALADL1) 536439(NAALAD2)
SSC: 
100515250(NAALADL1) 100524386(NAALAD2) 397677(FOLH1)
ECB: 
100050187(NAALAD2) 100060132(FOLH1B)
MDO: 
100009990 100013563(NAALAD2) 100030670(NAALADL1)
SHR: 
100913549(NAALADL1) 100918727 100927408(NAALAD2)
OAA: 
100081512 100081595
GGA: 
419010(NAALAD2) 419011(FOLH1)
MGP: 
100550377 100550530(NAALAD2) 100550836
TGU: 
100223080 100225953(NAALAD2)
ACS: 
100551729 100564848(naalad2)
XLA: 
100037180 734632(naalad2)
XTR: 
594947(naalad2)
DRE: 
100000223(naaladl1) 393247
TRU: 
101065215 101077227
OLA: 
101166965
BFO: 
BRAFLDRAFT_250341 BRAFLDRAFT_285244
CIN: 
494355
SPU: 
579656 588466
ISC: 
IscW_ISCW022420
CBR: 
CBG00524 CBG08178
SMM: 
Smp_135510
NVE: 
NEMVE_v1g106355 NEMVE_v1g164727
HMG: 
100207461
TAD: 
TRIADDRAFT_32203 TRIADDRAFT_64334
AQU: 
100639227
ATH: 
AT3G54720(AMP1) AT4G07670 AT5G19740
ALY: 
ARALYDRAFT_485877 ARALYDRAFT_488867
GMX: 
100797366 100798131 100817485 100818733
CSV: 
101221361
RCU: 
RCOM_0423040 RCOM_1049320
POP: 
POPTR_1080900 POPTR_905420
VVI: 
100247495 100247933
OSA: 
4326550 4327207 4334380
DOSA: 
Os01t0740500-00(Os01g0740500) Os01t0743300-01(Os01g0743300) Os03t0790600-01(Os03g0790600)
BDI: 
100822748 100823266 100823514 100825924
SBI: 
SORBI_01g005710(SORBIDRAFT_01g005710) SORBI_03g034150(SORBIDRAFT_03g034150) SORBI_03g034260(SORBIDRAFT_03g034260)
ZMA: 
100147738(vp8) 100280900
SMO: 
SELMODRAFT_119241(AMP1C-2) SELMODRAFT_134963(AMP1A-2) SELMODRAFT_402884(AMP1D2-1) SELMODRAFT_421595(AMP1A-1) SELMODRAFT_61588(AMP1D3-1) SELMODRAFT_61589(AMP1D1-1) SELMODRAFT_73556(AMP1C-1) SELMODRAFT_81471(AMP1B-1) SELMODRAFT_86742(AMP1D1-2) SELMODRAFT_87649(AMP1D2-2) SELMODRAFT_88219(AMP1B-2)
PPP: 
PHYPADRAFT_141903
CME: 
CMT078C
KLA: 
KLLA0D16588g
PPA: 
PAS_chr3_0815
VPO: 
Kpol_1036p74
DHA: 
DEHA2F13684g
PIC: 
PICST_33956(VPS70) PICST_72031(GCP1)
LEL: 
LELG_02618
CAL: 
CaO19.7106 CaO19_7106(CaJ7_0024)
CDU: 
CD36_70380
YLI: 
YALI0B05258g
PAN: 
PODANSg5808
FGR: 
FG00925.1
SSL: 
SS1G_12100
BFU: 
BC1G_11103
ANI: 
AN4018.2
NFI: 
NFIA_020930
AFM: 
AFUA_1G03740
AOR: 
AOR_1_1728154(AO090003000972)
ANG: 
ANI_1_512164(An18g03980)
AFV: 
AFLA_028450
ACT: 
ACLA_030870
PCS: 
Pc16g07930
CIM: 
CIMG_04607
CPW: 
CPC735_049960 CPC735_071930
URE: 
UREG_01813
PNO: 
SNOG_00541
TML: 
GSTUM_00011706001
MPR: 
MPER_12788
MGL: 
MGL_4102
PIF: 
PITG_00289
RHA: 
RHA1_ro02185
SEN: 
SACE_0204 SACE_6113
ABA: 
Acid345_2466 Acid345_2819 Acid345_2823
ACA: 
ACP_0923
ACM: 
AciX9_1255
TSA: 
AciPR4_1765
SUS: 
Acid_4582
CTM: 
Cabther_A1283
RMR: 
Rmar_2092
CPI: 
Cpin_4009
SHG: 
Sph21_2671
CMR: 
Cycma_4791
DFE: 
Dfer_1367
SLI: 
Slin_0518
RSI: 
Runsl_1076
RBI: 
RB2501_12577
MRS: 
Murru_3206
GAU: 
GAU_0163
 » show all
Taxonomy
Reference
1  [PMID:9123729]
  Authors
Heston WD.
  Title
Characterization and glutamyl preferring carboxypeptidase function of prostate specific membrane antigen: a novel folate hydrolase.
  Journal
Urology. 49 (1997) 104-12.
  Organism
Homo sapiens [GN:hsa]
Reference
2  [PMID:9187245]
  Authors
Rawlings ND, Barrett AJ.
  Title
Structure of membrane glutamate carboxypeptidase.
  Journal
Biochim. Biophys. Acta. 1339 (1997) 247-52.
  Organism
Homo sapiens [GN:hsa]
Reference
3  [PMID:9685395]
  Authors
Halsted CH, Ling EH, Luthi-Carter R, Villanueva JA, Gardner JM, Coyle JT.
  Title
Folylpoly-gamma-glutamate carboxypeptidase from pig jejunum. Molecular characterization and relation to glutamate carboxypeptidase II.
  Journal
J. Biol. Chem. 273 (1998) 20417-24.
  Organism
Sus scofa [GN:ssc]
Reference
4  [PMID:9501243]
  Authors
Luthi-Carter R, Berger UV, Barczak AK, Enna M, Coyle JT.
  Title
Isolation and expression of a rat brain cDNA encoding glutamate carboxypeptidase II.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 95 (1998) 3215-20.
  Organism
Rattus norvegicus [GN:rno]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
9074-87-7
DBGET integrated database retrieval system