KEGG   ENZYME: 3.4.21.103Help
Entry
EC 3.4.21.103               Enzyme                                 

Name
physarolisin;
Dictyostelium discoideum aspartic proteinase;
Dictyostelium discoideum aspartic proteinase E;
Physarum flavicomum aspartic proteinase;
Physarum polycephalum acid proteinase;
Physarum aspartic proteinase;
physaropepsin
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Serine endopeptidases
BRITE hierarchy
Reaction(IUBMB)
Milk clotting activity. Preferential cleavage of Gly8!Ser in B chain of insulin most rapidly, followed by Leu11!Val, Cys(SO3H)19!Gly and Phe24!Phe. No action on Ac-Phe-Tyr(I)2.
Comment
Belongs in peptidase family S53. From the slime mold Physarum polycephalum. Is not inhibited by pepstatin, but is blocked by methyl 2-diazoacetamidohexanoate. Closely similar enzymes are found in Dictyostelium discoideum and P. flavicomum. Formerly included in EC 3.4.23.6.
History
EC 3.4.21.103 created 1992 as EC 3.4.23.27 (EC 3.4.23.6 created 1992 (EC 3.4.23.6 created 1961 as EC 3.4.4.17, transferred 1972 to EC 3.4.23.6, modified 1981 [EC 3.4.23.7, EC 3.4.23.8, EC 3.4.23.9, EC 3.4.23.10, EC 3.4.99.1, EC 3.4.99.15 and EC 3.4.99.25 all created 1972 and incorporated 1978], part incorporated 1992), transferred 2003 to EC 3.4.21.103
Reference
1
  Authors
Henney, H.R. and Tavana, G.
  Title
Purification and some properties of an intracellular acid (carboxyl) proteinase from differentiating haploid cells of Physarum flavicomum.
  Journal
Exp. Mycol. 6 (1982) 161-170.
Reference
2
  Authors
Murakami-Murofushi, K., Hiratsuka, A. and Ohta, J.
  Title
A novel acid protease from haploid amoebae of Physarum polycephalum, and its changes during mating and subsequent differentiation into diploid plasmodia.
  Journal
Cell Struct. Funct. 9 (1984) 311-315.
Reference
3
  Authors
North, M.J. and Whyte, A.
  Title
Purification and characterization of two acid proteinases from Dictyostelium discoideum.
  Journal
J. Gen. Microbiol. 130 (1984) 123-134.
Reference
4  [PMID:12673349]
  Authors
Wlodawer A, Li M, Gustchina A, Oyama H, Dunn BM, Oda K.
  Title
Structural and enzymatic properties of the sedolisin family of serine-carboxyl peptidases.
  Journal
Acta. Biochim. Pol. 50 (2003) 81-102.
Reference
5  [PMID:12589815]
  Authors
Nishii W, Ueki T, Miyashita R, Kojima M, Kim YT, Sasaki N, Murakami-Murofushi K, Takahashi K.
  Title
Structural and enzymatic characterization of physarolisin (formerly physaropepsin) proves that it is a unique serine-carboxyl proteinase.
  Journal
Biochem. Biophys. Res. Commun. 301 (2003) 1023-9.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
94949-28-7

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