KEGG   ENZYME: 3.4.21.111Help
Entry
EC 3.4.21.111               Enzyme                                 

Name
aqualysin 1;
caldolysin
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Serine endopeptidases
BRITE hierarchy
Reaction(IUBMB)
Exhibits low specificity towards esters of amino acids with small hydrophobic or aromatic residues at the P1 position
Comment
This enzyme from the extreme thermophile, Thermus aquaticus, is an alkaline serine peptidase. It has three subsites, S1, S2, and S3, in the substrate binding site. The preferred amino acids at the S1 site are Ala and Phe, at the S2 site are Ala and norleucine and at the S3 site are Phe and Ile [3]. These specificities are similar to those of EC 3.4.21.64 (peptidase K) and EC 3.4.21.62 (subtilisin BPN') [3]. The enzyme displays broad specificity for cleavage of insulin B-chain and hydrolyses elastin substrates such as succinyl-(Ala)n-p-nitroanilide (n = 1,2,3) and some peptide esters [1,3]. Belongs in peptidase family S8A.
History
EC 3.4.21.111 created 2006
Reference
1  [PMID:3162211]
  Authors
Matsuzawa H, Tokugawa K, Hamaoki M, Mizoguchi M, Taguchi H, Terada I, Kwon ST, Ohta T.
  Title
Purification and characterization of aqualysin I (a thermophilic alkaline serine protease) produced by Thermus aquaticus YT-1.
  Journal
Eur. J. Biochem. 171 (1988) 441-7.
  Sequence
Reference
2  [PMID:9882104]
  Authors
Tanaka T, Matsuzawa H, Kojima S, Kumagai I, Miura K, Ohta T.
  Title
P1 specificity of aqualysin I (a subtilisin-type serine protease) from Thermus aquaticus YT-1, using P1-substituted derivatives of Streptomyces subtilisin inhibitor.
  Journal
Biosci. Biotechnol. Biochem. 62 (1998) 2035-8.
Reference
3
  Authors
Tanaka, T., Matsuzawa, H. and Ohta, T.
  Title
Substrate specificity of aqualysin I, a bacterial thermophilic alkaline serine protease from Thermus aquaticus YT-1: Comparison with proteinase K, subtilisin BPN' and subtilisin Carlsberg.
  Journal
Biosci. Biotechnol. Biochem. 62 (1998) 2161-2165.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
88747-68-6

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