KEGG   ENZYME: 3.4.21.113Help
Entry
EC 3.4.21.113               Enzyme                                 

Name
pestivirus NS3 polyprotein peptidase;
border disease virus NS3 endopeptidase;
BDV NS3 endopeptidase;
bovine viral diarrhea virus NS3 endopeptidase;
BVDV NS3 endopeptidase;
classical swine fever virus NS3 endopeptidase;
CSFV NS3 endopeptidase;
p80
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Serine endopeptidases
BRITE hierarchy
Reaction(IUBMB)
Leu is conserved at position P1 for all four cleavage sites. Alanine is found at position P1' of the NS4A-NS4B cleavage site, whereas serine is found at position P1' of the NS3-NS4A, NS4B-NS5A and NS5A-NS5B cleavage sites
Comment
The polyprotein of noncytopathogenic pestiviruses is cleaved co- and post-translationally into at least 11 proteins (Npro, C, Erns, E1, E2, p7, NS2-3, NS4A, NS4B, NS5A, and NS5B) [2]. The genomes of cytopathogenic pestivirus strains express at least one additional protein, called NS3 (p80) [2]. This enzyme, which resides in the N-terminal region of NS3 (nonstructural protein 3), is essential for generation of its own C-terminus and for processing of the downstream cleavage sites, leading to the release of the pestivirus nonstructural proteins NS4A, NS4B, NS5A and NS5B [1,2]. Belongs in peptidase family S31.
History
EC 3.4.21.113 created 2006
Reference
1  [PMID:1651596]
  Authors
Wiskerchen M, Collett MS.
  Title
Pestivirus gene expression: protein p80 of bovine viral diarrhea virus is a proteinase involved in polyprotein processing.
  Journal
Virology. 184 (1991) 341-50.
Reference
2  [PMID:9188613]
  Authors
Tautz N, Elbers K, Stoll D, Meyers G, Thiel HJ.
  Title
Serine protease of pestiviruses: determination of cleavage sites.
  Journal
J. Virol. 71 (1997) 5415-22.
Reference
3  [PMID:9188600]
  Authors
Xu J, Mendez E, Caron PR, Lin C, Murcko MA, Collett MS, Rice CM.
  Title
Bovine viral diarrhea virus NS3 serine proteinase: polyprotein cleavage sites, cofactor requirements, and molecular model of an enzyme essential for pestivirus replication.
  Journal
J. Virol. 71 (1997) 5312-22.
Reference
4  [PMID:10915606]
  Authors
Tautz N, Kaiser A, Thiel HJ.
  Title
NS3 serine protease of bovine viral diarrhea virus: characterization of active site residues, NS4A cofactor domain, and protease-cofactor interactions.
  Journal
Virology. 273 (2000) 351-63.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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