Entry |
|
Name |
kallikrein 8;
KLK8;
PRSS19;
human kallikrein 8;
hK8;
mK8;
ovasin;
tumor-associated differentially expressed gene 14;
TADG-14;
NP;
neuropsin
|
Class |
Hydrolases;
Acting on peptide bonds (peptidases);
Serine endopeptidases
 |
Reaction(IUBMB) |
Cleavage of amide substrates following the basic amino acids Arg or Lys at the P1 position, with a preference for Arg over Lys
|
Comment |
The enzyme is activated by removal of an N-terminal prepropeptide [2,4]. The highest amidolytic activity is observed using Boc-Val-Pro-Arg!7-amido-4-methylcoumarin, which is a substrate of alpha-thrombin [2,4]. Substrates lacking basic amino acids in the P1 position are not cleaved [4]. The enzyme degrades casein, fibronectin, gelatin, collagen type IV, fibrinogen, and high-molecular-mass kininogen [3] and is associated with diseases such as ovarian cancer and Alzheimer's disease [4]. Belongs in peptidase family S1A.
|
History |
EC 3.4.21.118 created 2006
|
Orthology |
|
Genes |
HSA: | | PTR: | | GGO: | | PON: | | NLE: | | MCC: | | MCF: | | CSAB: | | RRO: | | RBB: | | CJC: | | SBQ: | | MMU: | | RNO: | | CGE: | | NGI: | | HGL: | | CCAN: | | OCU: | | TUP: | | CFA: | | AML: | | UMR: | | ORO: | | FCA: | | PTG: | | AJU: | | BTA: | | BOM: | | BIU: | | PHD: | | CHX: | | OAS: | | SSC: | | CFR: | | CDK: | | ECB: | | EPZ: | | EAI: | | MYB: | | MYD: | | HAI: | | RSS: | | PALE: | | LAV: | | TMU: | | SHR: | | CJO: | | NCC: | | XMA: | | PRET: | | LCF: | | SASA: | | » show all
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Reference |
|
Authors |
Chen ZL, Yoshida S, Kato K, Momota Y, Suzuki J, Tanaka T, Ito J, Nishino H, Aimoto S, Kiyama H, et al. |
Title |
Expression and activity-dependent changes of a novel limbic-serine protease gene in the hippocampus. |
Journal |
J. Neurosci. 15 (1995) 5088-97. |
Sequence |
|
Reference |
|
Authors |
Shimizu C, Yoshida S, Shibata M, Kato K, Momota Y, Matsumoto K, Shiosaka T, Midorikawa R, Kamachi T, Kawabe A, Shiosaka S. |
Title |
Characterization of recombinant and brain neuropsin, a plasticity-related serine protease. |
Journal |
J. Biol. Chem. 273 (1998) 11189-96. |
Sequence |
|
Reference |
|
Authors |
Rajapakse S, Ogiwara K, Takano N, Moriyama A, Takahashi T. |
Title |
Biochemical characterization of human kallikrein 8 and its possible involvement in the degradation of extracellular matrix proteins. |
Journal |
FEBS. Lett. 579 (2005) 6879-84. |
Sequence |
|
Reference |
|
Authors |
Kishi T, Cloutier SM, Kundig C, Deperthes D, Diamandis EP. |
Title |
Activation and enzymatic characterization of recombinant human kallikrein 8. |
Journal |
Biol. Chem. 387 (2006) 723-31. |
Other DBs |
ExplorEnz - The Enzyme Database: IUBMB Enzyme Nomenclature: ExPASy - ENZYME nomenclature database: BRENDA, the Enzyme Database: CAS: 171715-15-4 |