KEGG   ENZYME: 3.4.21.25Help
Entry
EC 3.4.21.25                Enzyme                                 

Name
cucumisin;
euphorbain;
solanain;
hurain;
tabernamontanain
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Serine endopeptidases
BRITE hierarchy
Reaction(IUBMB)
Hydrolysis of proteins with broad specificity
Comment
From the sarcocarp of the musk melon (Cucumis melo). In peptidase family S8 (subtilisin family). Other endopeptidases from plants, which are less well characterized but presumably of serine-type, include euphorbain from Euphorbia cerifera [6], solanain from horse-nettle Solanum elaeagnifolium [1], hurain from Hura crepitans [2] and tabernamontanain from Tabernamontana grandiflora [3].
History
EC 3.4.21.25 created 1978 (EC 3.4.21.56 created 1972 as EC 3.4.99.7 transferred 1989 to EC 3.4.21.56, deleted 1992; EC 3.4.99.9 created 1972 deleted 1992; EC 3.4.99.21 created 1972 deleted 1992; EC 3.4.99.23 created 1972 deleted 1992; all covered by EC 3.4.21.25)
Reference
1
  Authors
Greenberg, D.M. and Winnick, T.
  Title
Plant proteases. I. Activation-inhibition reactions.
  Journal
J. Biol. Chem. 135 (1940) 761-773.
Reference
2
  Authors
Jaffe, W.G.
  Title
Hurain, a new plant protease from Hura crepitans.
  Journal
J. Biol. Chem. 149 (1943) 1-7.
Reference
3
  Authors
Jaffe, W.G.
  Title
A new vegetable proteolytic enzyme of the papain class.
  Journal
Rev. Brasil Biol. 3 (1943) 149-157.
Reference
4  [PMID:5423]
  Authors
Kaneda M, Tominaga N.
  Title
Isolation and characterization of a proteinase from the sarcocarp of melon fruit.
  Journal
J. Biochem. (Tokyo). 78 (1975) 1287-96.
Reference
5  [PMID:6427203]
  Authors
Kaneda M, Ohmine H, Yonezawa H, Tominaga N.
  Title
Amino acid sequence around the reactive serine of cucumisin from melon fruit.
  Journal
J. Biochem. (Tokyo). 95 (1984) 825-9.
Reference
6
  Authors
Lynn, K.R. and Clevette-Radford, N.A.
  Title
Two proteases from the latex of Elaeophorbia drupifera.
  Journal
Phytochemistry 24 (1985) 2843-2845.
Reference
7
  Authors
Kaneda, N., Minematsu, Y., Powers, J.C. and Tominaga, N.
  Title
Specificity of cucumisin in hydrolysis of peptide thiobenzyl esters.
  Journal
Agric. Biol. Chem. 50 (1986) 1075-1076.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
82062-89-3

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