KEGG   ENZYME: 3.4.21.32Help
Entry
EC 3.4.21.32                Enzyme                                 

Name
brachyurin;
Uca pugilator collagenolytic proteinase;
crab protease I;
crab protease II
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Serine endopeptidases
BRITE hierarchy
Reaction(IUBMB)
Hydrolysis of proteins, with broad specificity for peptide bonds. Native collagen is cleaved about 75% of the length of the molecule from the N-terminus. Low activity on small molecule substrates of both trypsin and chymotrypsin
Comment
From hepatopancreas of the fiddler crab, Uca pugilator. In peptidase family S1 (trypsin family). Other serine endopeptidases that degrade collagen, but are not listed separately here, include a second endopeptidase from Uca pugilator [4], digestive enzymes from other decapod crustacea [5,6], and an enzyme from the fungus Entomophthora coronata [1].
History
EC 3.4.21.32 created 1978
Reference
1  [PMID:219780]
  Authors
Hurion N, Fromentin H, Keil B.
  Title
Specificity of the collagenolytic enzyme from the fungus Entomophthora coronata: comparison with the bacterial collagenase from Achromobacter iophagus.
  Journal
Arch. Biochem. Biophys. 192 (1979) 438-45.
Reference
2
  Authors
Grant, G.A., Eisen, A.Z. and Bradshaw, R.A.
  Title
Collagenolytic protease from fiddler crab (Uca pugilator).
  Journal
Methods Enzymol. 80 (1981) 722-734.
Reference
3  [PMID:6756469]
  Authors
Welgus HG, Grant GA, Jeffrey JJ, Eisen AZ.
  Title
Substrate specificity of the collagenolytic serine protease from Uca pugilator: studies with collagenous substrates.
  Journal
Biochemistry. 21 (1982) 5183-9.
Reference
4  [PMID:6305411]
  Authors
Welgus HG, Grant GA.
  Title
Degradation of collagen substrates by a trypsin-like serine protease from the fiddler crab Uca pugilator.
  Journal
Biochemistry. 22 (1983) 2228-33.
Reference
5  [PMID:2154979]
  Authors
Klimova OA, Borukhov SI, Solovyeva NI, Balaevskaya TO, Strongin AYa.
  Title
The isolation and properties of collagenolytic proteases from crab hepatopancreas.
  Journal
Biochem. Biophys. Res. Commun. 166 (1990) 1411-20.
Reference
6  [PMID:1963309]
  Authors
Lu PJ, Liu HC, Tsai IH.
  Title
The midgut trypsins of shrimp (Penaeus monodon). High efficiency toward native protein substrates including collagens.
  Journal
Biol. Chem. Hoppe. Seyler. 371 (1990) 851-9.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
848900-32-3

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