KEGG   ENZYME: 3.4.21.99Help
Entry
EC 3.4.21.99                Enzyme                                 

Name
spermosin
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Serine endopeptidases
BRITE hierarchy
Reaction(IUBMB)
Hydrolyses arginyl bonds, preferably with Pro in the P2 position
Comment
The enzyme from the ascidian (Prochordate) Halocynthia roretzi is localized in the sperm head, and released during sperm activation. A proline-rich region is involved in binding to the vitelline coat of the egg. Belongs in peptidase family S1 (trypsin family).
History
EC 3.4.21.99 created 2001
Orthology
K20751  
spermosin
Genes
CIN: 
Taxonomy
Reference
1  [PMID:6365918]
  Authors
Sawada H, Yokosawa H, Ishii S.
  Title
Purification and characterization of two types of trypsin-like enzymes from sperm of the ascidian (Prochordata) Halocynthia roretzi. Evidence for the presence of spermosin, a novel acrosin-like enzyme.
  Journal
J. Biol. Chem. 259 (1984) 2900-4.
  Sequence
Reference
2  [PMID:6381175]
  Authors
Sawada H, Yokosawa H, Someno T, Saino T, Ishii S.
  Title
Evidence for the participation of two sperm proteases, spermosin and acrosin, in fertilization of the ascidian, Halocynthia roretzi: inhibitory effects of leupeptin analogs on enzyme activities and fertilization.
  Journal
Dev. Biol. 105 (1984) 246-9.
  Sequence
Reference
3  [PMID:8670234]
  Authors
Sawada H, Iwasaki K, Kihara-Negishi F, Ariga H, Yokosawa H.
  Title
Localization, expression, and the role in fertilization of spermosin, an ascidian sperm trypsin-like protease.
  Journal
Biochem. Biophys. Res. Commun. 222 (1996) 499-504.
  Sequence
Reference
4  [PMID:8914083]
  Authors
Sawada H, Someno T.
  Title
Substrate specificity of ascidian sperm trypsin-like proteases, spermosin and acrosin.
  Journal
Mol. Reprod. Dev. 45 (1996) 240-3.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
89925-67-7

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