KEGG   ENZYME: 3.4.22.32Help
Entry
EC 3.4.22.32                Enzyme                                 

Name
stem bromelain;
bromelain;
pineapple stem bromelain
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Cysteine endopeptidases
BRITE hierarchy
Reaction(IUBMB)
Broad specificity for cleavage of proteins, but strong preference for Z-Arg-Arg!NHMec amongst small molecule substrates
Comment
The most abundant of the cysteine endopeptidases of the stem of the pineapple plant, Ananas comosus. Distinct from the bromelain found in the pineapple fruit (EC 3.4.22.33). Scarcely inhibited by chicken cystatin and also very slowly inactivated by {glossary/E64::E-64}. In peptidase family C1 (papain family).
History
EC 3.4.22.32 created 1965 as EC 3.4.4.24, transferred 1972 to EC 3.4.22.4, part transferred 1992 to EC 3.4.22.32
Reference
1
  Authors
Brocklehurst, K., Willenbrock, F. and Salih, E.
  Title
Cysteine proteinases.
  Journal
In: Neuberger, A. and Brocklehurst, K. (Eds.), New Comprehensive Biochemistry: Hydrolytic Enzymes, Elsevier, Amsterdam, 1987, p. 39-158.
Reference
2  [PMID:3196029]
  Authors
Rowan AD, Buttle DJ, Barrett AJ.
  Title
Ananain: a novel cysteine proteinase found in pineapple stem.
  Journal
Arch. Biochem. Biophys. 267 (1988) 262-70.
Reference
3  [PMID:2714443]
  Authors
Ritonja A, Rowan AD, Buttle DJ, Rawlings ND, Turk V, Barrett AJ.
  Title
Stem bromelain: amino acid sequence and implications for weak binding of cystatin.
  Journal
FEBS. Lett. 247 (1989) 419-24.
Reference
4  [PMID:2327970]
  Authors
Rowan AD, Buttle DJ, Barrett AJ.
  Title
The cysteine proteinases of the pineapple plant.
  Journal
Biochem. J. 266 (1990) 869-75.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
37189-34-7

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