KEGG   ENZYME: 3.4.22.49Help
Entry
EC 3.4.22.49                Enzyme                                 
Name
separase;
separin
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Cysteine endopeptidases
BRITE hierarchy
Reaction(IUBMB)
All bonds known to be hydrolysed by this endopeptidase have arginine in P1 and an acidic residue in P4. P6 is often occupied by an acidic residue or by an hydroxy-amino-acid residue, the phosphorylation of which enhances cleavage
Comment
In both budding yeast and human cells, cleavage of the cohesin subunit Scc1 by separase is required for sister chromatid separation in mitosis. Budding yeast separase is also known to cleave the Rec8 subunit of a meiotic cohesin complex and the kinetochore protein Slk19. Type example of peptidase family C50.
Orthology
K02365  
separase
Genes
HSA: 
9700(ESPL1)
PTR: 
451937(ESPL1)
PPS: 
100986315(ESPL1)
GGO: 
101147294(ESPL1)
PON: 
100444516(ESPL1)
MCC: 
702136(ESPL1)
MMU: 
105988(Espl1)
RNO: 
315330(Espl1)
CFA: 
607879(ESPL1)
AML: 
100467596(ESPL1)
FCA: 
101084118(ESPL1)
BTA: 
506740(ESPL1)
SSC: 
100153121(ESPL1)
ECB: 
100063779(ESPL1)
MDO: 
100032809(ESPL1)
SHR: 
100921010(ESPL1)
GGA: 
425536(ESPL1)
ACS: 
100566013(espl1)
XTR: 
100158636(espl1)
DRE: 
797406(espl1)
TRU: 
101077884
OLA: 
101174584
BFO: 
BRAFLDRAFT_123958
CIN: 
100182879
SPU: 
576865
DME: 
Dmel_CG10583(Sse)
DPO: 
Dpse_GA10414
DAN: 
Dana_GF10451
DER: 
Dere_GG14118
DPE: 
Dper_GL11963
DSE: 
Dsec_GM13904
DSI: 
Dsim_GD13181
DWI: 
Dwil_GK16876(Dwil_Sse)
DYA: 
Dyak_GE20543
DGR: 
Dgri_GH15013
DMO: 
Dmoj_GI13175
DVI: 
Dvir_GJ11950(Dvir_Sse)
AGA: 
AgaP_AGAP001503
AAG: 
AaeL_AAEL008116
CQU: 
CpipJ_CPIJ011580
AME: 
100576647
API: 
100162113
ISC: 
IscW_ISCW017453
CEL: 
CELE_Y47G6A.12(sep-1)
CBR: 
CBG14956(Cbr-sep-1)
BMY: 
Bm1_17065
TSP: 
Tsp_02125
SMM: 
Smp_167810
NVE: 
NEMVE_v1g241520
ATH: 
AT4G22970(ESP)
ALY: 
ARALYDRAFT_492569
GMX: 
100791010 100805306
MTR: 
MTR_2g007790
CSV: 
101203087
RCU: 
RCOM_1374500
POP: 
POPTR_756563
VVI: 
100259948
OSA: 
4330866
DOSA: 
Os02t0770700-01(Os02g0770700)
BDI: 
100825113
SBI: 
SORBI_04g007853(SORBIDRAFT_04g007853) SORBI_04g034460(SORBIDRAFT_04g034460)
SMO: 
SELMODRAFT_405667 SELMODRAFT_442839
PPP: 
PHYPADRAFT_161712
CRE: 
CHLREDRAFT_12539(ESP1)
VCN: 
VOLCADRAFT_96349(espA)
OLU: 
OSTLU_119551(Cut1)
OTA: 
Ot02g02030(cut1)
SCE: 
YGR098C(ESP1)
AGO: 
AGOS_AAR089C
KLA: 
KLLA0D15136g
LTH: 
KLTH0E05478g
PPA: 
PAS_chr1-4_0541
ZRO: 
ZYRO0C12672g
CGR: 
CAGL0G03223g
NCS: 
NCAS_0E01080(NCAS0E01080)
NDI: 
NDAI_0G01210(NDAI0G01210)
TPF: 
TPHA_0D04010(TPHA0D04010)
TBL: 
TBLA_0C02250(TBLA0C02250)
TDL: 
TDEL_0F03240(TDEL0F03240)
KAF: 
KAFR_0K01610(KAFR0K01610)
DHA: 
DEHA2D01958g
PIC: 
PICST_28228(ESP1)
PGU: 
PGUG_04920
LEL: 
LELG_01443
CAL: 
CaO19.10864(ESP1) CaO19.3356(ESP1)
CTP: 
CTRG_04463
CDU: 
CD36_01530
COT: 
CORT_0B10500
YLI: 
YALI0C08960g
CLU: 
CLUG_00834
NCR: 
NCU00205
SMP: 
SMAC_02224(esp1)
PAN: 
PODANSg5443
TTT: 
THITE_2146478
MTM: 
MYCTH_2117988
MGR: 
MGG_06245
FGR: 
FG05504.1
NHE: 
NECHADRAFT_46823
VAL: 
VDBG_06747
SSL: 
SS1G_07342
BFU: 
BC1G_04716
ANI: 
AN8783.2
NFI: 
NFIA_077600
AFM: 
AFUA_5G09710
AOR: 
AOR_1_494174(AO090005000276)
ANG: 
ANI_1_1636064(An07g03090)
AFV: 
AFLA_075140
ACT: 
ACLA_012860
PCS: 
Pc12g05830
CIM: 
CIMG_04712
CPW: 
CPC735_071130
PBL: 
PAAG_05691
URE: 
UREG_01885
ABE: 
ARB_01287
TVE: 
TRV_01158
AJE: 
HCAG_04674
PNO: 
SNOG_00996
PTE: 
PTT_18381
ZTR: 
MYCGRDRAFT_92316
TML: 
GSTUM_00009703001
SPO: 
SPCC5E4.04(cut1)
CNE: 
CNB00660
CNB: 
CNBB5040
CGI: 
CGB_B9080W
LBC: 
LACBIDRAFT_307095 LACBIDRAFT_307111 LACBIDRAFT_317505 LACBIDRAFT_317789
MPR: 
MPER_01539
CCI: 
CC1G_02409
SCM: 
SCHCODRAFT_67598
UMA: 
UM03262.1
MGL: 
MGL_1370
PGR: 
PGTG_03501
EHI: 
EHI_120330(182.t00017)
EDI: 
EDI_265930
CPV: 
cgd7_2760
CHO: 
Chro.70313
PTM: 
GSPATT00003810001
TBR: 
Tb927.1.3120
TCR: 
507837.10 508405.80
LMA: 
LMJF_20_1680
LIF: 
LINJ_20_1650
LDO: 
LDBPK_201650
LMI: 
LMXM_20_1680
LBZ: 
LBRM_20_5840
TVA: 
TVAG_249880
TPS: 
THAPSDRAFT_260749
PIF: 
PITG_03497
 » show all
Taxonomy
Reference
1  [PMID:12194817]
  Authors
Waizenegger I, Gimenez-Abian JF, Wernic D, Peters JM.
  Title
Regulation of human separase by securin binding and autocleavage.
  Journal
Curr. Biol. 12 (2002) 1368-78.
  Organism
Homo sapiens [GN:hsa]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
351527-77-0
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