KEGG   ENZYME: 3.4.23.49Help
Entry
EC 3.4.23.49                Enzyme                                 

Name
omptin;
protease VII;
protease A;
gene ompT proteins;
ompT protease;
protein a;
Pla;
OmpT
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Aspartic endopeptidases
BRITE hierarchy
Reaction(IUBMB)
Has a virtual requirement for Arg in the P1 position and a slightly less stringent preference for this residue in the P1' position, which can also contain Lys, Gly or Val.
Comment
A product of the ompT gene of Escherichia coli, and associated with the outer membrane. Omptin shows a preference for cleavage between consecutive basic amino acids, but is capable of cleavage when P1' is a non-basic residue [5,7]. Belongs in peptidase family A26.
History
EC 3.4.23.49 created 1993 as EC 3.4.21.87, transferred 2006 to EC 3.4.23.49
Orthology
K01355  
omptin
Genes
ECO: 
b0565(ompT)
ECJ: 
JW0554(ompT)
ECD: 
EBW: 
BWG_0436(ompT)
ECE: 
ECS: 
ECF: 
ETW: 
ECSP_1567(ompT)
ELX: 
EOJ: 
EOI: 
EOH: 
ECG: 
EOK: 
ELR: 
ECC: 
c0652(ompT)
ECP: 
ECI: 
ECV: 
ECM: 
ECY: 
ECT: 
EOC: 
CE10_0562(ompT)
EUM: 
ECZ: 
ELN: 
ESE: 
EBR: 
ECB_00519(ompT)
EAB: 
EDH: 
EDJ: 
EIH: 
ELU: 
ELC: 
i14_0626(ompT)
ELD: 
i02_0626(ompT)
ELF: 
LF82_1565(ompT)
ECOA: 
ECOI: 
ECOJ: 
ECOO: 
EAL: 
SES: 
YRU: 
BD65_376(ompT)
EPY: 
EpC_02590(ompT)
EPR: 
EAM: 
EAMY_0249(ompT1)
EAY: 
ERJ: 
ENL: 
CRO: 
ROD_20151(ompT)
EBT: 
PFQ: 
REL: 
 » show all
Taxonomy
Reference
1  [PMID:3278297]
  Authors
Grodberg J, Lundrigan MD, Toledo DL, Mangel WF, Dunn JJ.
  Title
Complete nucleotide sequence and deduced amino acid sequence of the ompT gene of Escherichia coli K-12.
  Journal
Nucleic. Acids. Res. 16 (1988) 1209.
  Sequence
[eco:b0565]
Reference
2  [PMID:3056908]
  Authors
Sugimura K, Nishihara T.
  Title
Purification, characterization, and primary structure of Escherichia coli protease VII with specificity for paired basic residues: identity of protease VII and OmpT.
  Journal
J. Bacteriol. 170 (1988) 5625-32.
  Sequence
[eco:b0565]
Reference
3  [PMID:1603076]
  Authors
Hanke C, Hess J, Schumacher G, Goebel W.
  Title
Processing by OmpT of fusion proteins carrying the HlyA transport signal during secretion by the Escherichia coli hemolysin transport system.
  Journal
Mol. Gen. Genet. 233 (1992) 42-8.
Reference
4
  Authors
Dekker, N.
  Title
Omptin.
  Journal
In: Barrett, A.J., Rawlings, N.D. and Woessner, J.F. (Eds.), Handbook of Proteolytic Enzymes, 2nd ed., Elsevier, London, 2004, p. 212-216.
Reference
5  [PMID:11566868]
  Authors
Vandeputte-Rutten L, Kramer RA, Kroon J, Dekker N, Egmond MR, Gros P.
  Title
Crystal structure of the outer membrane protease OmpT from Escherichia coli suggests a novel catalytic site.
  Journal
EMBO. J. 20 (2001) 5033-9.
  Sequence
[eco:b0565]
Reference
6  [PMID:11576541]
  Authors
Kramer RA, Vandeputte-Rutten L, de Roon GJ, Gros P, Dekker N, Egmond MR.
  Title
Identification of essential acidic residues of outer membrane protease OmpT supports a novel active site.
  Journal
FEBS. Lett. 505 (2001) 426-30.
  Sequence
[eco:b0565]
Reference
7  [PMID:15317797]
  Authors
McCarter JD, Stephens D, Shoemaker K, Rosenberg S, Kirsch JF, Georgiou G.
  Title
Substrate specificity of the Escherichia coli outer membrane protease OmpT.
  Journal
J. Bacteriol. 186 (2004) 5919-25.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
150770-86-8

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