KEGG   ENZYME: 3.4.24.27Help
Entry
EC 3.4.24.27                Enzyme                                 

Name
thermolysin;
Bacillus thermoproteolyticus neutral proteinase;
thermoase;
thermoase Y10;
TLN
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Metalloendopeptidases
BRITE hierarchy
Reaction(IUBMB)
Preferential cleavage: !Leu > !Phe
Comment
A thermostable extracellular metalloendopeptidase containing four calcium ions. Enzymes that may be species variants of thermolysin are reported from Micrococcus caseolyticus [4] and Aspergillus oryzae [5]. Type example of peptidase family M4. Closely related but distinct enzymes are aeromonolysin, pseudolysin, bacillolysin, aureolysin and mycolysin
History
EC 3.4.24.27 created 1972 as EC 3.4.24.4, part transferred 1992 to EC 3.4.24.27
Orthology
K08603  
thermolysin
Genes
BAN: 
BAR: 
BAT: 
BCY: 
BWE: 
GKA: 
ESI: 
EAT: 
FRA: 
HAU: 
Taxonomy
Reference
1  [PMID:5954368]
  Authors
Ohta Y, Ogura Y, Wada A.
  Title
Thermostable protease from thermophilic bacteria. I. Thermostability, physiocochemical properties, and amino acid composition.
  Journal
J. Biol. Chem. 241 (1966) 5919-25.
  Organism
Bacillus thermoproteolyticus
Reference
2  [PMID:4967801]
  Authors
Morihara K, Tsuzuki H, Oka T.
  Title
Comparison of the specificities of various neutral proteinases from microorganisms.
  Journal
Arch. Biochem. Biophys. 123 (1968) 572-88.
Reference
3  [PMID:5823940]
  Authors
Latt SA, Holmquist B, Vallee BL.
  Title
Thermolysin: a zinc metalloenzyme.
  Journal
Biochem. Biophys. Res. Commun. 37 (1969) 333-9.
  Organism
Bacillus thermoproteolyticus
Reference
4  [PMID:5551628]
  Authors
Desmazeaud MJ, Hermier JH.
  Title
[Specificity of the nuclear protease produced by Micrococcus caseolyticus]
  Journal
Eur. J. Biochem. 19 (1971) 51-5.
  Organism
Micrococcus caseolyticus
Reference
5  [PMID:5004124]
  Authors
Morihara K, Tsuzuki H.
  Title
Comparative study of various neutral proteinases from microorganisms: specificity with oligopeptides.
  Journal
Arch. Biochem. Biophys. 146 (1971) 291-6.
Reference
6
  Authors
Titani, K., Hermodson, M.
  Title
A., Ericson, L. H., Walsh, K. A. and Neurath, H. Amino-acid sequence of thermolysin.
  Journal
Nature New Biol. 238 (1972) 35-37.
Reference
7
  Authors
Matthews, B.
  Title
W. Structural basis of the action of thermolysin and related zinc peptidases.
  Journal
Acc. Chem. Res. 21 (1988) 333-340.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
9073-78-3

DBGET integrated database retrieval system