| Entry |
|
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| Name |
deuterolysin;
Penicillium roqueforti protease II;
microbial neutral proteinase II;
acid metalloproteinase;
neutral proteinase II;
Penicillium roqueforti metalloproteinase
|
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| Class |
Hydrolases;
Acting on peptide bonds (peptidases);
Metalloendopeptidases
 |
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| Reaction(IUBMB) |
Preferential cleavage of bonds with hydrophobic residues in P1'; also Asn3!Gln and Gly8!Ser bonds in insulin B chain
|
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| Comment |
Proteolytic activity found in Penicillium roqueforti [4], P. caseicolum [4], Aspergillus sojae [3] and A. oryzae [1,5]. Optimum pH of 5 for digesting various proteins. Strong action on protamine and histones. Insensitive to phosphoramidon. About 20 kDa. A distinct Aspergillus sojae endopeptidase is larger and has a neutral pH optimum. Type example of peptidase family M35. Formerly included in EC 3.4.24.4
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| Reference |
1 |
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| Authors |
Nakadai, T., Nasuno, S. and Iguchi, N. |
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| Title |
Purification and properties of neutral proteinase II from Aspergillus oryzae. |
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| Journal |
Agric. Biol. Chem. 37 (1973) 2703-2708. |
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| Reference |
|
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| Authors |
Gripon JC, Hermier J. |
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| Title |
[The proteolytic system of Penicillium roqueforti. III. - Purification, properties and specificity of a protease inhibited by E.D.T.A] |
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| Journal |
Biochimie. 56 (1974) 1323-32. |
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| Organism |
Penicillium roqueforti |
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| Reference |
3 |
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| Authors |
Sekine, H. |
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| Title |
, Neutral proteinases I and II of Aspergillus sojae action on various substrates. |
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| Journal |
Agric. Biol. Chem. 40 (1976) 703-709. |
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| Reference |
|
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| Authors |
Gripon JC, Auberger B, Lenoir J. |
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| Title |
Metalloproteases from Penicillium caseicolum and P. roqueforti: comparison of specificity and chemical characterization. |
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| Journal |
Int. J. Biochem. 12 (1980) 451-5. |
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| Organism |
Penicillium roqueforti, Penicillium caseicolum |
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| Reference |
5 |
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| Authors |
Vaganova, T.I., Ivanova, N.M. and Stepanov, V.M. |
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| Title |
Isolation and properties of the "acid" metalloproteinase from Aspergillus oryzae. |
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| Journal |
Biochemistry (Mosc) 53 (1988) 1171-1178. |
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| Other DBs |
ExplorEnz - The Enzyme Database: IUBMB Enzyme Nomenclature: ExPASy - ENZYME nomenclature database: BRENDA, the Enzyme Database: CAS: 247028-11-1 |
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