KEGG   ENZYME: 3.4.24.39Help
Entry
EC 3.4.24.39                Enzyme                                 

Name
deuterolysin;
Penicillium roqueforti protease II;
microbial neutral proteinase II;
acid metalloproteinase;
neutral proteinase II;
Penicillium roqueforti metalloproteinase
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Metalloendopeptidases
BRITE hierarchy
Reaction(IUBMB)
Preferential cleavage of bonds with hydrophobic residues in P1'; also Asn3!Gln and Gly8!Ser bonds in insulin B chain
Comment
Proteolytic activity found in Penicillium roqueforti [4], P. caseicolum [4], Aspergillus sojae [3] and A. oryzae [1,5]. Optimum pH of 5 for digesting various proteins. Strong action on protamine and histones. Insensitive to phosphoramidon. About 20 kDa. A distinct Aspergillus sojae endopeptidase is larger and has a neutral pH optimum. Type example of peptidase family M35. Formerly included in EC 3.4.24.4
History
EC 3.4.24.39 created 1972 as EC 3.4.24.4, part transferred 1992 to EC 3.4.24.39
Orthology
K19305  
deuterolysin
Genes
NCR: 
NTE: 
NEUTE1DRAFT113287(NEUTE1DRAFT_113287)
SMP: 
MGR: 
TMN: 
FGR: 
FPU: 
NHE: 
TRE: 
MAW: 
MAJ: 
CMT: 
VAL: 
VDA: 
ELA: 
SSL: 
BFU: 
ANI: 
AFM: 
AOR: 
AOR_1_224164(AO090001000135) AOR_1_818024(AO090010000493)
AFV: 
ACT: 
NFI: 
PCS: 
CIM: 
CPW: 
PBL: 
PBN: 
URE: 
ABE: 
TVE: 
AJE: 
PNO: 
PTE: 
ZTR: 
PFJ: 
NPA: 
ADL: 
GTR: 
MPR: 
MRR: 
CCI: 
SCM: 
ABP: 
AGABI1DRAFT113068(AGABI1DRAFT_113068)
ABV: 
AGABI2DRAFT188701(AGABI2DRAFT_188701)
WSE: 
 » show all
Taxonomy
Reference
1
  Authors
Nakadai, T., Nasuno, S. and Iguchi, N.
  Title
Purification and properties of neutral proteinase II from Aspergillus oryzae.
  Journal
Agric. Biol. Chem. 37 (1973) 2703-2708.
Reference
2  [PMID:4219726]
  Authors
Gripon JC, Hermier J.
  Title
[The proteolytic system of Penicillium roqueforti. III. - Purification, properties and specificity of a protease inhibited by E.D.T.A]
  Journal
Biochimie. 56 (1974) 1323-32.
Reference
3
  Authors
Sekine, H.
  Title
, Neutral proteinases I and II of Aspergillus sojae action on various substrates.
  Journal
Agric. Biol. Chem. 40 (1976) 703-709.
Reference
4  [PMID:6998789]
  Authors
Gripon JC, Auberger B, Lenoir J.
  Title
Metalloproteases from Penicillium caseicolum and P. roqueforti: comparison of specificity and chemical characterization.
  Journal
Int. J. Biochem. 12 (1980) 451-5.
Reference
5
  Authors
Vaganova, T.I., Ivanova, N.M. and Stepanov, V.M.
  Title
Isolation and properties of the "acid" metalloproteinase from Aspergillus oryzae.
  Journal
Biochemistry (Mosc) 53 (1988) 1171-1178.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
247028-11-1

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