KEGG   ENZYME: 3.4.24.44Help
Entry
EC 3.4.24.44                Enzyme                                 

Name
atrolysin E;
Crotalus atrox metalloendopeptidase e;
hemorrhagic toxin e
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Metalloendopeptidases
BRITE hierarchy
Reaction(IUBMB)
Cleavage of Asn3!Gln, Ser9!His and Ala14!Leu bonds in insulin B chain and Tyr14!Gln and Thr8!Ser in A chain. Cleaves type IV collagen at Ala73!Gln in alpha1(IV) and at Gly7!Leu in alpha2(IV)
Comment
A 25.7 kDa hemorrhagic endopeptidase from the venom of the western diamondback rattlesnake (Crotalus atrox) that digests basement membrane components, including the triple helix of type IV collagen. Such action is believed to contribute to the hemorrhagic property by weakening capillary walls. In peptidase family M12 (astacin family)
History
EC 3.4.24.44 created 1992
Reference
1  [PMID:210790]
  Authors
Bjarnason JB, Tu AT.
  Title
Hemorrhagic toxins from Western diamondback rattlesnake (Crotalus atrox) venom: isolation and characterization of five toxins and the role of zinc in hemorrhagic toxin e.
  Journal
Biochemistry. 17 (1978) 3395-404.
Reference
2  [PMID:6351911]
  Authors
Bjarnason JB, Fox JW.
  Title
Proteolytic specificity and cobalt exchange of hemorrhagic toxin e, a zinc protease isolated from the venom of the western diamondback rattlesnake (Crotalus atrox).
  Journal
Biochemistry. 22 (1983) 3770-8.
Reference
3  [PMID:2374521]
  Authors
Baramova EN, Shannon JD, Bjarnason JB, Fox JW.
  Title
Identification of the cleavage sites by a hemorrhagic metalloproteinase in type IV collagen.
  Journal
Matrix. 10 (1990) 91-7.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
172306-51-3

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