KEGG   ENZYME: 3.5.1.100Help
Entry
EC 3.5.1.100                Enzyme                                 

Name
(R)-amidase;
R-stereospecific amidase;
R-amidase
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
BRITE hierarchy
Sysname
(R)-piperazine-2-carboxamide amidohydrolase
Reaction(IUBMB)
(1) (R)-piperazine-2-carboxamide + H2O = (R)-piperazine-2-carboxylic acid + NH3 [RN:R09647];
(2) beta-alaninamide + H2O = beta-alanine + NH3 [RN:R09648]
Reaction(KEGG)
Substrate
(R)-piperazine-2-carboxamide [CPD:C19778];
H2O [CPD:C00001];
beta-alaninamide [CPD:C19779]
Product
(R)-piperazine-2-carboxylic acid [CPD:C19780];
NH3 [CPD:C00014];
beta-alanine [CPD:C00099]
Comment
In addition (R)-piperidine-3-carboxamide is hydrolysed to (R)-piperidine-3-carboxylic acid and NH3, and (R)-N-tert-butylpiperazine-2-carboxamide is hydrolysed to (R)-piperazine-2-carboxylic acid and tert-butylamine with lower activity. The enzyme does not act on the other amide substrates which are hydrolysed by EC 3.5.1.4 (amidase).
History
EC 3.5.1.100 created 2009, modified 2011
Orthology
K18540  
(R)-amidase
Genes
SLQ: 
PAE: 
PAEV: 
N297_3722(ramA)
PAEI: 
N296_3722(ramA)
PAU: 
PAP: 
PAG: 
PAF: 
PNC: 
PDK: 
PSG: 
PRP: 
PAEP: 
PAER: 
PAEM: 
PAEL: 
PAES: 
PAEU: 
PAEG: 
PAEC: 
M802_3719(ramA)
PAEO: 
M801_3587(ramA)
PPU: 
PPG: 
PPT: 
PPUH: 
PPUD: 
PMOS: 
PPUU: 
PFL: 
PPRC: 
PFO: 
PEN: 
PFV: 
PDR: 
PRE: 
PMON: 
PMOT: 
PKC: 
PKB_3494(ramA)
PCH: 
PALK: 
PSW: 
CSA: 
TAU: 
PSE: 
BVI: 
BUR: 
BCN: 
BCH: 
BCM: 
BCJ: 
BCAL1380(ramA)
BCEN: 
DM39_1273(ramA)
BAM: 
BMU: 
BMJ: 
BCT: 
BCED: 
DM42_329(ramA)
BGL: 
BGD: 
BUK: 
DEL: 
MSG: 
MSB: 
MSN: 
MSH: 
NOU: 
TAR: 
 » show all
Taxonomy
Reference
1  [PMID:15066183]
  Authors
Komeda H, Harada H, Washika S, Sakamoto T, Ueda M, Asano Y
  Title
A novel R-stereoselective amidase from Pseudomonas sp. MCI3434 acting on piperazine-2-tert-butylcarboxamide.
  Journal
Eur. J. Biochem. 271 (2004) 1580-90.
  Sequence
[up:Q75SP7]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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