KEGG   ENZYME: 3.5.1.100Help
Entry
EC 3.5.1.100                Enzyme                                 

Name
(R)-amidase;
R-stereospecific amidase;
R-amidase
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
BRITE hierarchy
Sysname
(R)-piperazine-2-carboxamide amidohydrolase
Reaction(IUBMB)
(1) (R)-piperazine-2-carboxamide + H2O = (R)-piperazine-2-carboxylic acid + NH3 [RN:R09647];
(2) beta-alaninamide + H2O = beta-alanine + NH3 [RN:R09648]
Reaction(KEGG)
Substrate
(R)-piperazine-2-carboxamide [CPD:C19778];
H2O [CPD:C00001];
beta-alaninamide [CPD:C19779]
Product
(R)-piperazine-2-carboxylic acid [CPD:C19780];
NH3 [CPD:C00014];
beta-alanine [CPD:C00099]
Comment
In addition (R)-piperidine-3-carboxamide is hydrolysed to (R)-piperidine-3-carboxylic acid and NH3, and (R)-N-tert-butylpiperazine-2-carboxamide is hydrolysed to (R)-piperazine-2-carboxylic acid and tert-butylamine with lower activity. The enzyme does not act on the other amide substrates which are hydrolysed by EC 3.5.1.4 (amidase).
History
EC 3.5.1.100 created 2009, modified 2011
Reference
1  [PMID:15066183]
  Authors
Komeda H, Harada H, Washika S, Sakamoto T, Ueda M, Asano Y
  Title
A novel R-stereoselective amidase from Pseudomonas sp. MCI3434 acting on piperazine-2-tert-butylcarboxamide.
  Journal
Eur. J. Biochem. 271 (2004) 1580-90.
  Organism
Pseudomonas sp.
  Sequence
[up:Q75SP7]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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