| Entry |
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| Name |
(R)-amidase;
R-stereospecific amidase;
R-amidase
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| Class |
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
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| Sysname |
(R)-piperazine-2-carboxamide amidohydrolase
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| Reaction(IUBMB) |
(1) (R)-piperazine-2-carboxamide + H2O = (R)-piperazine-2-carboxylic acid + NH3 [RN: R09647];
(2) beta-alaninamide + H2O = beta-alanine + NH3 [RN: R09648]
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| Reaction(KEGG) |
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| Substrate |
(R)-piperazine-2-carboxamide [CPD: C19778];
H2O [CPD: C00001];
beta-alaninamide [CPD: C19779]
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| Product |
(R)-piperazine-2-carboxylic acid [CPD: C19780];
NH3 [CPD: C00014];
beta-alanine [CPD: C00099]
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| Comment |
In addition (R)-piperidine-3-carboxamide is hydrolysed to (R)-piperidine-3-carboxylic acid and NH3, and (R)-N-tert-butylpiperazine-2-carboxamide is hydrolysed to (R)-piperazine-2-carboxylic acid and tert-butylamine with lower activity. The enzyme does not act on the other amide substrates which are hydrolysed by EC 3.5.1.4 (amidase).
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| Reference |
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| Authors |
Komeda H, Harada H, Washika S, Sakamoto T, Ueda M, Asano Y |
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| Title |
A novel R-stereoselective amidase from Pseudomonas sp. MCI3434 acting on piperazine-2-tert-butylcarboxamide. |
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| Journal |
Eur. J. Biochem. 271 (2004) 1580-90. |
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| Organism |
Pseudomonas sp. |
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| Other DBs |
ExplorEnz - The Enzyme Database: IUBMB Enzyme Nomenclature: ExPASy - ENZYME nomenclature database: BRENDA, the Enzyme Database: |
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