KEGG   ENZYME: 3.5.1.103Help
Entry
EC 3.5.1.103                Enzyme                                 

Name
N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-D-glucopyranoside deacetylase;
MshB
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
BRITE hierarchy
Sysname
1-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol acetylhydrolase
Reaction(IUBMB)
1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + H2O = 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + acetate [RN:R09651]
Reaction(KEGG)
Substrate
1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol;
H2O [CPD:C00001]
Product
1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol [CPD:C19702];
acetate [CPD:C00033]
Comment
This enzyme is considered the key enzyme and rate limiting step in the mycothiol biosynthesis pathway [1]. In addition to acetylase activity, the enzyme possesses weak activity of EC 3.5.1.115, mycothiol S-conjugate amidase, and shares sequence similarity with that enzyme [2]. The enzyme requires a divalent transition metal ion for activity, believed to be Zn2+ [3].
History
EC 3.5.1.103 created 2010
Orthology
K15525  N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-D-glucopyranoside deacetylase
Genes
MTU: Rv1170(mshB)
MTV: RVBD_1170
MTC: MT1207
MRA: MRA_1181(mshB)
MTF: TBFG_11195
MTB: TBMG_02811
MTK: TBSG_02825
MTZ: TBXG_002791
MTG: MRGA327_07375
MTI: MRGA423_07300
MTUR: CFBS_1249(mshB)
MTO: MTCTRI2_1202(mshB)
MTD: UDA_1170(mshB)
MTN: ERDMAN_1313(mshB)
MTUC: J113_08195
MTUE: J114_06325
MTUH: I917_08310
MTUL: TBHG_01154
MTUT: HKBT1_1247(mshB)
MTUU: HKBT2_1253(mshB)
MTQ: HKBS1_1251(mshB)
MBO: BQ2027_MB1203(mshB)
MBB: BCG_1233(mshB)
MBT: JTY_1206(mshB)
MBM: BCGMEX_1205(mshB)
MBX: BCGT_1002
MAF: MAF_11890(mshB)
MCE: MCAN_11811(mshB)
MCQ: BN44_11308(mshB)
MCV: BN43_30237(mshB)
MCX: BN42_21035(mshB)
MCZ: BN45_30230(mshB)
MMIC: RN08_1314
MLE: ML1495
MLB: MLBr01495
MPA: MAP_2613c
MAO: MAP4_1205
MAVU: RE97_05965
MAV: MAV_1310
MAVD: NF84_05815
MAVR: LA63_05950
MAVA: LA64_05965
MIT: OCO_12210
MIA: OCU_12170
MID: MIP_01950
MYO: OEM_12360
MIR: OCQ_12230
MMAL: CKJ54_05930(mshB)
MUL: MUL_1017(mshB)
MMC: Mmcs_4034
MKM: Mkms_4109
MJL: Mjls_4264
MMI: MMAR_4283(mshB)
MMAE: MMARE11_40980(mshB)
MMM: W7S_05960
MLI: MULP_04469(mshB)
MHAD: B586_07400
MSG: MSMEI_5001(mshB)
MVA: Mvan_4535
MGI: Mflv_2165
MFT: XA26_45410(mshB)
MPHL: MPHLCCUG_04078(mshB)
MVQ: MYVA_4310
MAB: MAB_1316
MABB: MASS_1315
MABO: NF82_06630
MCHE: BB28_06490
MSTE: MSTE_01284(mshB)
MJD: JDM601_1159(mshB)
ASD: AS9A_3372(mshB)
CGL: NCgl1055(Cgl1100)
CGB: cg1250
CGU: WA5_1055(MshB)
CGT: cgR_1183
CGM: cgp_1250(mshB)
CGJ: AR0_06000
CEF: CE1158
CDI: DIP0971
CDP: CD241_0879(mshB)
CDH: CDB402_0847(mshB)
CDT: CDHC01_0879(mshB)
CDE: CDHC02_0878(mshB)
CDR: CDHC03_0875(mshB)
CDA: CDHC04_0885(mshB)
CDZ: CD31A_0977(mshB)
CDB: CDBH8_0939(mshB)
CDS: CDC7B_0884(mshB)
CDD: CDCE8392_0875(mshB)
CDW: CDPW8_0935(mshB)
CDV: CDVA01_0842(mshB)
CDIP: ERS451417_00874(mshB)
CJK: jk1397
CUR: cu0660
CUA: CU7111_0649(mshB)
CAR: cauri_0978(mshB)
CPU: cpfrc_00769(mshB)
CPL: Cp3995_0781(mshB)
CPG: Cp316_0797(mshB)
CPP: CpP54B96_0780(mshB)
CPK: Cp1002_0769(mshB)
CPQ: CpC231_0769(mshB)
CPX: CpI19_0769(mshB)
CPZ: CpPAT10_0768(mshB)
COR: Cp267_0803(mshB)
COP: Cp31_0777(mshB)
COD: Cp106_0753(mshB)
COS: Cp4202_0759(mshB)
COI: CpCIP5297_0785(mshB)
COE: Cp258_0774(mshB)
COU: Cp162_0768(mshB)
CPSE: CPTA_01324
CPSU: CPTB_00208
CPSF: CPTC_01314
CRD: CRES_1472(mshB)
CUN: Cul210932_0856(mshB)
CUS: CulFRC11_0822(mshB)
CUQ: Cul210931_0819(mshB)
CUZ: Cul05146_0878(mshB)
CUJ: CUL131002_0834(mshB)
CVA: CVAR_1875(mshB)
CTER: A606_07630
CGY: CGLY_06475(mshB)
COA: DR71_1804(mshB)
CSX: CSING_05390(mshB)
CMQ: B840_04480(mshB)
CKU: UL82_07825(mshB)
CCJ: UL81_04480(mshB)
CMV: CMUST_05905(mshB)
CEI: CEPID_04680(mshB)
CTED: CTEST_04805(mshB)
CUT: CUTER_04055(mshB)
CDX: CDES_05320(mshB)
CSP: WM42_0392
CPHO: CPHO_07915
CGV: CGLAU_04845(mshB)
CAQU: CAQU_04700
CAMG: CAMM_08565
NFA: NFA_47570(mshB)
NFR: ERS450000_05171(mshB_2)
NCY: NOCYR_4504(mshB)
NNO: NONO_c35200(mshB1) NONO_c64660(mshB2)
NSR: NS506_01888(mshB)
NTP: CRH09_33565(mshB)
RER: RER_41900(mshB)
REY: O5Y_19590
ROP: ROP_59960(mshB)
REQ: REQ_14110(mshB)
RHB: NY08_5012
RFA: A3L23_03070(mshB_2)
RHS: A3Q41_00258(mshB_1)
RRZ: CS378_23315(mshB)
RHU: A3Q40_02336(mshB)
RQI: C1M55_20680(mshB)
GBR: Gbro_3329
TPR: Tpau_1146
SRT: Srot_0833
DIT: C3V38_15820(mshB)
DIZ: CT688_09935(mshB)
SCO: SCO5126(SC9E12.11)
SMA: SAVERM_3138(mshB)
SGR: SGR_2405(mshB)
SCT: SCAT_3982
SFA: Sfla_2168
SBH: SBI_04101
SDV: BN159_3280(mshB)
SALB: XNR_1686
SALS: SLNWT_2192
STRP: F750_4644(mshB)
SLV: SLIV_12750(mshB)
SGU: SGLAU_21965(mshB)
STRE: GZL_03583
SLD: T261_2938
STRM: M444_22410
SAMB: SAM23877_4908(mshB)
SPRI: SPRI_2752
SRW: TUE45_01129(mca_1) TUE45_05617(mshB_2)
SLE: sle_26120(sle_26120)
SRN: A4G23_03743(mshB)
SNR: SNOUR_15865(mshB)
STRD: NI25_13515
SMAL: SMALA_5078
SLAU: SLA_4949
SALJ: SMD11_2437
SLX: SLAV_03235(mca2) SLAV_14000(mshB2)
KSK: KSE_48330(mshB)
ARM: ART_1903
KRH: KRH_08220(mshB)
JDE: Jden_0794
LMOI: VV02_20450
XCE: Xcel_0963
IDO: I598_1357(mshB)
CFL: Cfla_0988
CFI: Celf_2899
SERJ: SGUI_2990
BLIN: BLSMQ_1667
DCO: SAMEA4475696_2002(mshB_2)
PFRE: RM25_2107
MPH: MLP_43290(mshB)
NCA: Noca_1157
NDK: I601_2148(mshB_1)
KFL: Kfla_5531
PSIM: KR76_20790
AEZ: C3E78_12970(mshB)
TFU: Tfu_0486
NDA: Ndas_3908
NAL: B005_1085(mshB)
NGV: CDO52_07605(mshB)
TCU: Tcur_1131
SRO: Sros_8449
ACE: Acel_1868
NML: Namu_4309
GOB: Gobs_1084
MMAR: MODMU_1135
KRA: Krad_1134
AMD: AMED_8023
AMN: RAM_41220
AMM: AMES_7904
AMZ: B737_7904
AOI: AORI_6834
AJA: AJAP_04940(mshB)
AMYC: CU254_32665(mshB)
PDX: Psed_1005
PSEA: WY02_21210
PSEE: FRP1_22120
PSEH: XF36_20365
AMI: Amir_0766
APRE: CNX65_03790(mshB)
SESP: BN6_08510(mshB)
ACTI: UA75_04500
ACAD: UA74_04395
AHG: AHOG_04250(mshB1)
ACTA: C1701_07565(mshB)
ALL: CRK61671
SAQ: Sare_4145
MIL: ML5_2969
AMS: AMIS_73690(mshB)
ASE: ACPL_7649(mshB)
ACTN: L083_7369(mshB)
AFS: AFR_38780
ACTS: ACWT_7519
PLAB: C6361_36360(mshB)
PLAT: C6W10_10260(mshB)
SNA: Snas_5506
TBI: Tbis_3036
AEY: CDG81_17765(mshB)
ABAM: B1s21122_01305(mshB)
DEW: DGWBC_0129(mshB)
DFO: Dform_00451(mshB)
 » show all
Taxonomy
Reference
1  [PMID:12724395]
  Authors
Rawat M, Kovacevic S, Billman-Jacobe H, Av-Gay Y
  Title
Inactivation of mshB, a key gene in the mycothiol biosynthesis pathway in Mycobacterium smegmatis.
  Journal
Microbiology. 149 (2003) 1341-9.
Reference
2  [PMID:11092856]
  Authors
Newton GL, Av-Gay Y, Fahey RC
  Title
N-Acetyl-1-D-myo-inosityl-2-amino-2-deoxy-alpha-D-glucopyranoside deacetylase (MshB) is a key enzyme in mycothiol biosynthesis.
  Journal
J. Bacteriol. 182 (2000) 6958-63.
  Sequence
[mtu:Rv1170] [msm:MSMEG_5129]
Reference
3  [PMID:12958317]
  Authors
Maynes JT, Garen C, Cherney MM, Newton G, Arad D, Av-Gay Y, Fahey RC, James MN
  Title
The crystal structure of 1-D-myo-inosityl 2-acetamido-2-deoxy-alpha-D-glucopyranoside deacetylase (MshB) from Mycobacterium tuberculosis reveals a zinc hydrolase with a lactate dehydrogenase fold.
  Journal
J. Biol. Chem. 278 (2003) 47166-70.
  Sequence
[mtu:Rv1170]
Other DBs
ExplorEnz - The Enzyme Database: 3.5.1.103
IUBMB Enzyme Nomenclature: 3.5.1.103
ExPASy - ENZYME nomenclature database: 3.5.1.103
BRENDA, the Enzyme Database: 3.5.1.103

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