KEGG   ENZYME: 3.5.1.103Help
Entry
EC 3.5.1.103                Enzyme                                 

Name
N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-D-glucopyranoside deacetylase;
MshB
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
BRITE hierarchy
Sysname
1-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol acetylhydrolase
Reaction(IUBMB)
1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + H2O = 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + acetate [RN:R09651]
Reaction(KEGG)
Substrate
1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol;
H2O [CPD:C00001]
Product
1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol [CPD:C19702];
acetate [CPD:C00033]
Comment
This enzyme is considered the key enzyme and rate limiting step in the mycothiol biosynthesis pathway [1]. In addition to acetylase activity, the enzyme possesses weak activity of EC 3.5.1.115, mycothiol S-conjugate amidase, and shares sequence similarity with that enzyme [2]. The enzyme requires a divalent transition metal ion for activity, believed to be Zn2+ [3].
History
EC 3.5.1.103 created 2010
Orthology
K15525  
N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-D-glucopyranoside deacetylase
Genes
MTU: 
Rv1170(mshB)
MTV: 
MTC: 
MRA: 
MRA_1181(mshB)
MTF: 
MTB: 
MTK: 
MTZ: 
MTG: 
MTI: 
MTE: 
MTUR: 
CFBS_1249(mshB)
MTL: 
MTO: 
MTD: 
UDA_1170(mshB)
MTN: 
MTJ: 
MTUB: 
MTUC: 
MTUE: 
MTX: 
MTUH: 
MTUL: 
MBO: 
Mb1203(mshB)
MBB: 
BCG_1233(mshB)
MBT: 
JTY_1206(mshB)
MBM: 
MBK: 
MAF: 
MAF_11890(mshB)
MCE: 
MCQ: 
MCV: 
MCX: 
MCZ: 
MLE: 
MLB: 
MPA: 
MAO: 
MAV: 
MIT: 
MIR: 
MIA: 
MID: 
MYO: 
MSM: 
MSG: 
MSA: 
MUL: 
MUL_1017(mshB)
MVA: 
MGI: 
MSP: 
MAB: 
MABB: 
MMV: 
MMC: 
MKM: 
MJL: 
MJD: 
MMI: 
MMAR_4283(mshB)
MRH: 
MMM: 
MCB: 
MLI: 
MKN: 
MNE: 
ASD: 
AS9A_3372(mshB)
CGL: 
NCgl1055(Cgl1100)
CGB: 
CGU: 
WA5_1055(MshB)
CGT: 
CGS: 
CGG: 
CGM: 
cgp_1250(mshB)
CEF: 
CDI: 
CDP: 
CDH: 
CDT: 
CDE: 
CDR: 
CDA: 
CDZ: 
CDB: 
CDS: 
CDD: 
CDW: 
CDV: 
CJK: 
CUR: 
CUA: 
CAR: 
CKP: 
CPU: 
CPL: 
CPG: 
CPP: 
CPK: 
CPQ: 
CPX: 
CPZ: 
COR: 
COP: 
Cp31_0777(mshB)
COD: 
COS: 
COI: 
COE: 
COU: 
CRD: 
CRES_1472(mshB)
CUL: 
CUC: 
CUE: 
CVA: 
CVAR_1875(mshB)
CHN: 
CCN: 
CTER: 
CMD: 
CAZ: 
CFN: 
CCG: 
CVT: 
CGY: 
NFA: 
NCY: 
NBR: 
NNO: 
NONO_c35200(mshB1) NONO_c64660(mshB2)
RHA: 
RER: 
RER_41900(mshB)
REY: 
ROP: 
ROP_59960(mshB)
ROA: 
REQ: 
REQ_14110(mshB)
RPY: 
GBR: 
GPO: 
GOR: 
TPR: 
SRT: 
SCO: 
SCO5126(SC9E12.11)
SMA: 
SAV_3138(mshB)
SGR: 
SGR_2405(mshB)
SCB: 
SSX: 
SVL: 
SCT: 
SCY: 
SFA: 
SBH: 
SHY: 
SHO: 
SVE: 
SDV: 
SALB: 
STRP: 
F750_4644(mshB)
SFI: 
SCI: 
SRC: 
SALU: 
KSK: 
KSE_48330(mshB)
RSA: 
KRH: 
KRH_08220(mshB)
RMU: 
RDN: 
JDE: 
XCE: 
IVA: 
SKE: 
CFL: 
CFI: 
CGA: 
ICA: 
PFR: 
PPC: 
MPH: 
MLP_43290(mshB)
NCA: 
KFL: 
TFU: 
NDA: 
NAL: 
B005_1085(mshB)
TCU: 
SRO: 
FRA: 
FRE: 
FRI: 
FAL: 
FSY: 
ACE: 
NML: 
GOB: 
BSD: 
MMAR: 
KRA: 
SEN: 
SVI: 
TBI: 
AMD: 
AMN: 
AMM: 
AMZ: 
AOI: 
PDX: 
AMI: 
SESP: 
BN6_08510(mshB)
KAL: 
STP: 
SAQ: 
MAU: 
MIL: 
VMA: 
AMS: 
ASE: 
ACPL_7649(mshB)
ACTN: 
L083_7369(mshB)
AFS: 
CAI: 
SNA: 
DGO: 
DPD: 
 » show all
Taxonomy
Reference
1  [PMID:12724395]
  Authors
Rawat M, Kovacevic S, Billman-Jacobe H, Av-Gay Y
  Title
Inactivation of mshB, a key gene in the mycothiol biosynthesis pathway in Mycobacterium smegmatis.
  Journal
Microbiology. 149 (2003) 1341-9.
  Organism
Mycobacterium smegmatis
Reference
2  [PMID:11092856]
  Authors
Newton GL, Av-Gay Y, Fahey RC
  Title
N-Acetyl-1-D-myo-inosityl-2-amino-2-deoxy-alpha-D-glucopyranoside deacetylase (MshB) is a key enzyme in mycothiol biosynthesis.
  Journal
J. Bacteriol. 182 (2000) 6958-63.
  Organism
Mycobacterium tuberculosis, Mycobacterium smegmatis
  Sequence
[mtu:Rv1170] [msm:MSMEG_5129]
Reference
3  [PMID:12958317]
  Authors
Maynes JT, Garen C, Cherney MM, Newton G, Arad D, Av-Gay Y, Fahey RC, James MN
  Title
The crystal structure of 1-D-myo-inosityl 2-acetamido-2-deoxy-alpha-D-glucopyranoside deacetylase (MshB) from Mycobacterium tuberculosis reveals a zinc hydrolase with a lactate dehydrogenase fold.
  Journal
J. Biol. Chem. 278 (2003) 47166-70.
  Organism
Mycobacterium tuberculosis
  Sequence
[mtu:Rv1170]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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